Patent US20030105397A1 successfully refreshed from Google Patents.
US4603111A
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Preparation of biocatalysts in bead form
Assignee: Hoechst AG
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Filing: 1983-10-06
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Granted: 1986-07-29
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Expires: 2003-10-06
Abstract
Abstract Biocatalysts in bead form which contain microorganisms are particularly advantageously obtained by radical polymerization of acrylamide with methylene-bis-acrylamide in aqueous-organic phase suspension when the organic phase is a highly fluorinated carbon compound. The biocatalysts thus obtained have high stability and activity and are suitable for biotechnical processes.
Inventors
- 👤 Reinhold Keller
- 👤 Gunter Siegemund
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:14
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C08; C08F; C08F220/00; C08F220/02; C08F220/52; C08F220/54; C08F220/56
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
58 words
A process for the preparation of a biocatalyst in bead form, comprising: mixing microbial cells, acrylamide and, methylene-bis-acrylamide in an aqueous phase with an organic phase consisting essentially of a highly fluorinated carbon compound to form a suspension, and thereafter polymerizing the acrylamide and the methylene-bis-acrylamide monomers in the suspension to produce polymer beads containing microbial cells therein.
Claim 2
Dependent Claim
58 words
A process for the preparation of a biocatalyst in bead form, comprising: mixing microbial cells, acrylamide and, methylene-bis-acrylamide in an aqueous phase with an organic phase consisting essentially of a highly fluorinated carbon compound to form a suspension, and thereafter polymerizing the acrylamide and the methylene-bis-acrylamide monomers in the suspension to produce polymer beads containing microbial cells therein.
Claim 3
Dependent Claim
28 words
The process of claim 1 wherein the highly fluorinated carbon compound is one in which at least half of all the bonds in the molecule are carbon/fluorine bonds.
Claim 4
Dependent Claim
16 words
The process of claim 2 wherein the highly fluorinated carbon compound is perfluoro-1-nonene, 2,3-di-chloroperfluoro-2-methylpentane, or perfluro-nonane.
Claim 5
Dependent Claim
15 words
The process as claimed in claim 1, wherein the polymerization is started with a persulfate.
Claim 6
Dependent Claim
16 words
The process as claimed in claim 1, wherein the polymerization temperature is not above room temperature.
Claim 7
Dependent Claim
16 words
The process as claimed in claim 1, wherein the polymerization temperature is 5° to 20° C.
Claim 8
Dependent Claim
16 words
The process as claimed in claim 1, wherein the polymerization temperature is 10° to 15° C.
Claim 9
Dependent Claim
25 words
The process as claimed in claim 1, wherein the ratio of the volume of the aqueous phase to the organic phase is 1:3 to 1:15.
Claim 10
Dependent Claim
14 words
The process as claimed in claim 1, wherein the ratio is 1:6 to 1:10.
Claim 11
Dependent Claim
27 words
The process as claimed in claim 1, wherein the highly fluorinated compound is a liquid having a boiling point under normal pressure of 40° to 300° C.
Claim 12
Dependent Claim
27 words
The process as claimed in claim 1, wherein the highly fluorinated compound is a liquid having a boiling point under normal pressure of 90° to 200° C.
Claim 13
Dependent Claim
27 words
The process as claimed in claim 1, wherein the highly fluorinated carbon compound is a liquid perhalogenated highly fluorinated hydrocarbon wherein the halogen is other than fluorine.
Claim 14
Dependent Claim
18 words
The process as claimed in claim 1, wherein the highly fluorinated carbon compound is a liquid perfluorinated hydrocarbon.
Claim 15
Dependent Claim
13 words
The process as claimed in claim 1, wherein a polymerization initiator is added.
Claim 16
Dependent Claim
13 words
The process as claimed in claim 1, wherein a surface-active compound is added.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-07-28 | 1990-01-27 | 1990-07-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-07-28 | 1994-01-27 | 1994-07-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-07-28 | 1998-01-27 | 1998-07-29 | $7700.00 | Window Closed / Lapsed |