US4599304A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for monitoring activated cell subpopulations
Assignee: Becton Dickinson and Co
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Filing: 1983-10-07
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Granted: 1986-07-08
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Expires: 2003-10-07
Abstract
Abstract A method of distinguishing multiple subpopulations of a cell sample whereby resting and activated human natural killer cells subpopulations can be monitored. This method utilizes two monoclonal antibodies identified as anti-Leu 11 and anti-DR (or anti-Leu 10 or anti-transferrin receptor).
Inventors
- 👤 Lewis Lanier
- 👤 Joseph Phillips
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 09:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; G01N2800/00; G01N2800/52; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
165 words
A method for monitoring activated and non-activated natural killer cell subpopulations comprising: (a) providing a sample of cells containing a population of natural killer cells; (b) labelling selected cells of said sample with a first monoclonal antibody(s) coupled with a first fluorochrome label; (c) labelling selected cells of said sample with a second monoclonal antibody(s) coupled with a second fluorochrome label said first fluorochrome label and said second fluorochrome label having a detectable emission difference; one of said first or second monoclonal antibodies being anti-Leu 11 and the other of said first or second monoclonal antibodies being selected from the group consisting of anti-DR, anti-Leu 10 and anti-transferrin receptor; (d) providing excitation energy to excite said first fluorochrome label and said second fluorochrome label; (e) detecting the fluorescence emitted by the excited fluorochromes; and (f) distinguishing subpopulations of activated and non-activated natural killer cells relative to the detected fluorescence characteristics thereof, whereby the relative proportion of non-activated and activated natural killer cells can be monitored.
Claim 2
Independent Claim
165 words
A method for monitoring activated and non-activated natural killer cell subpopulations comprising: (a) providing a sample of cells containing a population of natural killer cells; (b) labelling selected cells of said sample with a first monoclonal antibody(s) coupled with a first fluorochrome label; (c) labelling selected cells of said sample with a second monoclonal antibody(s) coupled with a second fluorochrome label said first fluorochrome label and said second fluorochrome label having a detectable emission difference; one of said first or second monoclonal antibodies being anti-Leu 11 and the other of said first or second monoclonal antibodies being selected from the group consisting of anti-DR, anti-Leu 10 and anti-transferrin receptor; (d) providing excitation energy to excite said first fluorochrome label and said second fluorochrome label; (e) detecting the fluorescence emitted by the excited fluorochromes; and (f) distinguishing subpopulations of activated and non-activated natural killer cells relative to the detected fluorescence characteristics thereof, whereby the relative proportion of non-activated and activated natural killer cells can be monitored.
Claim 3
Dependent Claim
17 words
A method in accordance with claim 1 wherein said sample of cells is a peripheral blood sample.
Claim 4
Dependent Claim
35 words
A method in accordance with claim 2 wherein said blood sample is treated to provide a mononuclear cell fraction comprising substantially all of the lymphocytes including natural cells prior to step (b) of claim 1.
Claim 5
Dependent Claim
32 words
A method in accordance with claim 1 wherein said first fluorochrome label is selected from fluoroscein, rhodamine and a phycobiliprotein and said second fluorochrome is selected from Texas Red and a phycobiliprotein.
Claim 6
Dependent Claim
19 words
A method in accordance with claim 1 wherein said first fluorochrome is fluorescein and said second fluorochrome is phycoerythrin.
Claim 7
Dependent Claim
19 words
A method in accordance with claim 1 wherein said fluorescence detection is accomplished by means of a fluorescence microscope.
Claim 8
Dependent Claim
18 words
A method in accordance with claim 1 wherein said fluorescence is accomplished with a fluorescence activated cell sorter.
References Cited (Backward Citations)
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