US4596723A
Expired / Lapsed
US
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Immunoassay substrate
Assignee: Hitachi Chemical Diagnostics Inc
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Filing: 1984-05-02
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Granted: 1986-06-24
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Expires: 2004-05-02
Abstract
Abstract Preparation of an immunoassay gel substrate which includes the steps of depositing on an immobilizing support structure a liquid mass of a gel-forming emulsion which coalesces into a thick gel-like matrix film characterized by substantial non-agglomerated matrix homogeneity, treating the resulting film to alter matrix homogeneity by agglomerating particles which form part of the gel, and as a consequence of the treating step establishing the object gel. Treating may be performed either by subjecting the gel to multiple time-vigorous water-wash and dry cycles, by emersing the gel in water for a relatively long period of time, or by wetting the gel and then subjecting it to one or more freeze/thaw cycles.
Inventors
- 👤 Nancy K. Kaufman
- 👤 Richard A. Harte
- 👤 Anthony B. Chen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 04:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; Y; Y10; Y10T; Y10T428/00; Y10T428/25; Y10T428/254
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
106 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, particle-containing, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with such film being characterized by substantial particle-dispersion homogeneity, after drying of such a mass, and following coalescence thereof into such a film, treating the resulting film to alter such particle homogeneity by agglomerating such particles in the film, and by said treating and agglomerating, establishing the object gel.
Claim 2
Independent Claim
106 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, particle-containing, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with such film being characterized by substantial particle-dispersion homogeneity, after drying of such a mass, and following coalescence thereof into such a film, treating the resulting film to alter such particle homogeneity by agglomerating such particles in the film, and by said treating and agglomerating, establishing the object gel.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein said treating is performed by subjecting the film to a time-extensive water-immersion bath.
Claim 4
Dependent Claim
21 words
The method of claim 1, wherein said treating is performed by subjecting the film to multiple, time-successive, water-wash and dry cycles.
Claim 5
Dependent Claim
25 words
The method of claim 1, wherein said treating is performed by wetting the film, and by then subjecting it to at least one freeze/thaw cycle.
Claim 6
Dependent Claim
23 words
The method of claim 1, wherein said treating is performed by wetting the film, and by then subjecting it to plural freeze/thaw cycles.
Claim 7
Independent Claim
131 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, multiple-binding-site, non-sterically-hindered, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with such film being characterized by substantial particle-dispersion homogeneity, with different particles interconnected by elongated material strands, each of which carries plural, longitudinally distributed protein binding sites, after initial drying of such mass, and following coalescence thereof into such a film, treating the resulting film to alter particle homogeneity by agglomerating particles in the film, and as a consequence of said agglomerating, stretching such strands to spread apart the binding sites distributed therealong.
Claim 8
Independent Claim
131 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, multiple-binding-site, non-sterically-hindered, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with such film being characterized by substantial particle-dispersion homogeneity, with different particles interconnected by elongated material strands, each of which carries plural, longitudinally distributed protein binding sites, after initial drying of such mass, and following coalescence thereof into such a film, treating the resulting film to alter particle homogeneity by agglomerating particles in the film, and as a consequence of said agglomerating, stretching such strands to spread apart the binding sites distributed therealong.
Claim 9
Dependent Claim
19 words
The method of claim 6, wherein said treating is performed by subjecting the film to a time-extensive water-immersion bath.
Claim 10
Dependent Claim
21 words
The method of claim 6, wherein said treating is performed by subjecting the film to multiple, time-successive, water-wash and dry cycles.
Claim 11
Dependent Claim
25 words
The method of claim 6, wherein said treating is performed by wetting the film, and by then subjecting it to at least one freeze/thaw cycle.
Claim 12
Dependent Claim
23 words
The method of claim 6, wherein said treating is performed by wetting the film, and by then subjecting it to plural freeze/thaw cycles.
Claim 13
Independent Claim
78 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, multiple-binding-site, non-sterically-hindered, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with different particles in such film interconnected by elongated material strands that carry plural longitudinally distributed protein binding sites, and
Claim 14
Independent Claim
78 words
A method for preparing on an immobilizing support structure a three-dimensionally-extensive, water-swellable, multiple-binding-site, non-sterically-hindered, protein-binding gel for the subsequent reception and binding therein of a selected bindable protein, said method comprising depositing on such a structure a liquid mass of a gel-forming emulsion which, upon initial drying, is coalesceable into a thick gel-like film adhered to the structure, with different particles in such film interconnected by elongated material strands that carry plural longitudinally distributed protein binding sites, and
Claim 15
Dependent Claim
47 words
after initial drying of such a mass, and following coalescence thereof into such a film, elongating such strands between such connected particles, thus to increase the separation between adjacent binding sites distributed therealong to minimize steric hindrance in the film and to maximize the film's protein-binding capability.
Claim 16
Dependent Claim
47 words
after initial drying of such a mass, and following coalescence thereof into such a film, elongating such strands between such connected particles, thus to increase the separation between adjacent binding sites distributed therealong to minimize steric hindrance in the film and to maximize the film's protein-binding capability.
Claim 17
Dependent Claim
19 words
The method of claim 11, wherein said treating is performed by subjecting the film to a time-extensive water-immersion bath.
Claim 18
Dependent Claim
21 words
The method of claim 11, wherein said treating is performed by subjecting the film to multiple, time-successive, water-wash and dry cycles.
Claim 19
Dependent Claim
25 words
The method of claim 11, wherein said treating is performed by wetting the film, and by then subjecting it to at least one freeze/thaw cycle.
Claim 20
Dependent Claim
23 words
The method of claim 11, wherein said treating is performed by wetting the film, and by then subjecting it to plural freeze/thaw cycles.
References Cited (Backward Citations)
US4340564A
Cited
US4540660A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-23 | 1989-12-23 | 1990-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-23 | 1993-12-23 | 1994-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-23 | 1997-12-23 | 1998-06-24 | $7700.00 | Window Closed / Lapsed |