US4596035A
Expired / Lapsed
US
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Methods for enumerating 3-part white cell differential clusters
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1983-06-27
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Granted: 1986-06-17
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Expires: 2003-06-27
Abstract
Abstract Methods useful for differentiating and enumerating subpopulations of particles in a sample and more particularly relates to the enumeration of leukocyte subclasses. Enumeration is accomplished by utilizing two light scatter parameters and converting these parameters to a single parameter by employing a mathematical, nonlinear transformation.
Inventors
- 👤 Russell J. Gershman
- 👤 Irving L. Weiner
- 👤 Arthur C. Daniels
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 13:33
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N21/00; G01N21/17; G01N21/47; G01N2021/4704; G01N2021/4707; G01N2021/4711
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
162 words
A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; (e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and (f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.
Claim 2
Independent Claim
162 words
A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; (e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and (f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.
Claim 3
Dependent Claim
206 words
A medhod for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; (e) calculating a series of lines orthogonal to said trajectory pursuant to the nonlinear transformation: C(x,y)=(1-mX)bY 1/2 +aX 2 +hX+k where X equals the log of the right angle scatter measurement, Y equals the forward angle scatter measurement, and m, b, a, h, and k are constants chosen to subtantially optimize the orthogonality of these lines to said trajectory; and (f) counting the cells along each line of constant C whereby cellular subpopulations may be enumerated.
Claim 4
Dependent Claim
206 words
A medhod for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; (e) calculating a series of lines orthogonal to said trajectory pursuant to the nonlinear transformation: C(x,y)=(1-mX)bY 1/2 +aX 2 +hX+k where X equals the log of the right angle scatter measurement, Y equals the forward angle scatter measurement, and m, b, a, h, and k are constants chosen to subtantially optimize the orthogonality of these lines to said trajectory; and (f) counting the cells along each line of constant C whereby cellular subpopulations may be enumerated.
Claim 5
Dependent Claim
23 words
The method as provided in claim 2 wherein the sample contains leukocytes and the subpopulations to be counted comprise granulocytes, lymphocytes, and monocytes.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-16 | 1989-12-16 | 1990-06-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-16 | 1993-12-16 | 1994-06-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-16 | 1997-12-16 | 1998-06-17 | $7700.00 | Window Closed / Lapsed |