US4594318A
Expired / Lapsed
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Isolation and localization of DNA segments
Assignee: Massachusetts Institute of Technology
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Filing: 1981-04-29
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Granted: 1986-06-10
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Expires: 2001-04-29
Abstract
Abstract A process of isolating and identifying cloned DNA segments from defined regions of a chromosome by preparing a series of cell hybrids from a first species and a second species, which hybrids contain a chromosome or deletion mutant of a chromosome as the o The Government has rights in this invention pursuant to Contract Number CA-5-14051 awarded by the Department of Health and Human Services, Contract Number PCM 77-17747 awarded by the National Science Foundation, and Contract Number 1-713 awarded by the National Foundation-March of Dimes.
Inventors
- 👤 James F. Gusella
- 👤 David Housman
- 👤 Cheryl Keys
- 👤 Aviva Varsanyi-Breiner
- 👤 Theodore T. Puck
- 👤 Carol Jones
- 👤 Fa-Ten Kao
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:09
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12N; C12N15/00; C12N15/09; C12N15/10; C12Q1/6813; C12Q1/6827; C12Q1/6841
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
116 words
A process for the isolation of human repetitive DNA fragments comprising: (a) isolating 15 to 20 kilobase human DNA fragments; (b) inserting the human DNA fragments of step a into the DNA of lambda bacteriophage to form a clone; (c) screening the ligated phage DNA for clones containing human DNA; (d) isolating the DNA from the clones of step c containing human DNA; (e) fragmenting the isolated DNA of step d; (f) resolving the fragments of step e; (g) hybridizing the resolved fragments of step f with human DNA using molecular hybridizing conditions which only identify fragments with human repetitive DNA sequences, wherein said repetitive DNA fragments are isolated from and are unique to human chromosomes.
Claim 2
Dependent Claim
116 words
A process for the isolation of human repetitive DNA fragments comprising: (a) isolating 15 to 20 kilobase human DNA fragments; (b) inserting the human DNA fragments of step a into the DNA of lambda bacteriophage to form a clone; (c) screening the ligated phage DNA for clones containing human DNA; (d) isolating the DNA from the clones of step c containing human DNA; (e) fragmenting the isolated DNA of step d; (f) resolving the fragments of step e; (g) hybridizing the resolved fragments of step f with human DNA using molecular hybridizing conditions which only identify fragments with human repetitive DNA sequences, wherein said repetitive DNA fragments are isolated from and are unique to human chromosomes.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-06-09 | 1989-12-09 | 1990-06-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-06-09 | 1993-12-09 | 1994-06-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-06-09 | 1997-12-09 | 1998-06-10 | $7700.00 | Window Closed / Lapsed |