US4591499A
Expired / Lapsed
US
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Method for treatment and prevention of mastitis
Assignee: BOYER CLIFFORD M
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Filing: 1984-01-20
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Granted: 1986-05-27
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Expires: 2004-01-20
Abstract
Abstract A method for treating mastitis in ruminants by the administration of non-pathogenic Lactobacilli. The Lactobacilli are preferably administered in an oil emulsion which includes a water and an oil soluble emulsifier.
Inventors
- 👤 Leighton L. Linn
- 👤 Robert J. Mathiesen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 04:14
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K35/741; A61K35/744; A61K35/747; A61K39/00; A61K39/02; A61K39/09; A61K2039/55; A61K2039/552; A61K2039/555; A61K2039/55511; A61K2039/55566
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
66 words
A method of controlling mastitis in mammals, said method comprising: introducing into the mammary gland of said mammals a non-toxic amount of one or more non-pathogenic lactic-acid producing live bacteria wherein said amount is sufficient to control mastitis; allowing said bacteria to remain in said gland in a viable state until milk present in the gland is clabbered; and removing the clabbered milk from said gland.
Claim 2
Independent Claim
66 words
A method of controlling mastitis in mammals, said method comprising: introducing into the mammary gland of said mammals a non-toxic amount of one or more non-pathogenic lactic-acid producing live bacteria wherein said amount is sufficient to control mastitis; allowing said bacteria to remain in said gland in a viable state until milk present in the gland is clabbered; and removing the clabbered milk from said gland.
Claim 3
Dependent Claim
29 words
A method as set forth in claim 1, wherein said quantity of bacteria is sufficient to lower the pH of the gland to at least about 5.0 to 6.5.
Claim 4
Dependent Claim
17 words
A method as set forth in claim 1, wherein said bacteria is selected from the genus Lactobacillus.
Claim 5
Dependent Claim
18 words
A method as set forth in claim 3, wherein said bacteria is present in a non-toxic oil emulsion.
Claim 6
Dependent Claim
15 words
A method as set forth in claim 4, wherein each of said steps is repeated.
Claim 7
Dependent Claim
17 words
A method as set forth in claim 1, wherein said bacteria comprises Lactobacillus acidophilus and Lactobacillus casei.
Claim 8
Dependent Claim
23 words
A method as set forth in claim 1, wherein said bacteria is selected from the group consisting of Lactobacillus acidophilus and Lactobacillus casei.
Claim 9
Dependent Claim
26 words
A method as set forth in claim 1, wherein said introducing step comprises introducing an oil emulsion comprising both an oil soluble and water soluble ensulsifier.
Claim 10
Dependent Claim
22 words
A method as set forth in claim 8, wherein said emulsifiers, in admixture, comprises an HLB of from about 3 to 15.
Claim 11
Dependent Claim
17 words
A method as set forth in claim 8, wherein said oil emulsion comprises an unsaturated vegetable oil.
Claim 12
Dependent Claim
34 words
A method as set forth in claim 8, wherein the live bacteria count injected into said gland is within the range of approximately 1×10 3 to 1×10 9 CFU per cc of said emulsion.
Claim 13
Dependent Claim
37 words
A method as set forth in claim 1, wherein said introducing step comprises introducing approximately 6 to 15 cc of said bacteria having a live bacteria count of approximately 1×10 3 to 1×10 9 CFU per cc.
Claim 14
Independent Claim
96 words
A method of controlling mastitis in mammals distinguished by the presence of an udder, said method comprising: (a) introducing into said udder through the teat canal a mastitis controlling non-toxic quantity of an oil emulsion of one or more non-pathogenic live lactic acid producing bacteria, said bacteria being present in sufficient quantity to lower the pH of the udder to at least about 5.0 to 6.5; (b) allowing said bacteria to remain in said udder in a viable state until milk present in the udder is clabbered; (c) milking said udder to remove the clabbered milk.
Claim 15
Independent Claim
96 words
A method of controlling mastitis in mammals distinguished by the presence of an udder, said method comprising: (a) introducing into said udder through the teat canal a mastitis controlling non-toxic quantity of an oil emulsion of one or more non-pathogenic live lactic acid producing bacteria, said bacteria being present in sufficient quantity to lower the pH of the udder to at least about 5.0 to 6.5; (b) allowing said bacteria to remain in said udder in a viable state until milk present in the udder is clabbered; (c) milking said udder to remove the clabbered milk.
Claim 16
Dependent Claim
44 words
A method as set forth in claim 13, wherein said introducing step comprises introducing an oil emulsion of said bacteria, said emulsion comprising both oil soluble and water soluble emulsifiers, wherein the HLB of said emulsifiers, in admixture, is from about 3 to 15.
Claim 17
Dependent Claim
39 words
A method as set forth in claim 14, wherein said bacteria count is at least about 1×10 3 CFU per cc of said emulsion and said bacteria is selected from the group consisting of Lactobacillus acidophilus and Lactobacillus casei.
Claim 18
Dependent Claim
18 words
A method as set forth in claim 15, wherein said bacteria consists of Lactobacillus acidophilus and Lactobacillus casei.
Claim 19
Dependent Claim
28 words
A method as set forth in claim 16 wherein said emulsion comprises a suspension agent selected from the group consisting of glycerin, polyethylene glycol, polypropylene glycol and sorbitol.
Claim 20
Dependent Claim
16 words
A method as set forth in claim 17, wherein said oil comprises an unsaturated vegetable oil.
Claim 21
Dependent Claim
22 words
A method as set forth in claim 18, wherein said introducing step comprises introducing approximately 6 to 15 cc of said emulsion.
Claim 22
Dependent Claim
15 words
A method as set forth in claim 19 wherein steps (a) through (c) are repeated.
Claim 23
Independent Claim
98 words
A method of controlling mastitis in bovines, said method comprising: (a) introducing into the udder through the teat canal approximately 6 to 15 cc of a non-toxic oil emulsion of live bacteria selected from the group consisting of Lactobacillus acidophilus, and Lactobacillus casei, said live bacteria count being at least about 1×10 3 CFU per cc of said emulsion; (b) allowing said bacteria to remain in said udder in a viable state for approximately 8 to 12 hours thereby resulting in clabbering of the milk in the udder; and (c) milking said udder to remove the clabbered milk.
Claim 24
Independent Claim
98 words
A method of controlling mastitis in bovines, said method comprising: (a) introducing into the udder through the teat canal approximately 6 to 15 cc of a non-toxic oil emulsion of live bacteria selected from the group consisting of Lactobacillus acidophilus, and Lactobacillus casei, said live bacteria count being at least about 1×10 3 CFU per cc of said emulsion; (b) allowing said bacteria to remain in said udder in a viable state for approximately 8 to 12 hours thereby resulting in clabbering of the milk in the udder; and (c) milking said udder to remove the clabbered milk.
Claim 25
Dependent Claim
15 words
A method as set forth in claim 21, wherein steps (a) through (c) are repeated.
Claim 26
Dependent Claim
21 words
A method as set forth in claim 22, wherein said oil emulsion comprises a suspension agent and an unsaturated vegetable oil.
Claim 27
Dependent Claim
31 words
A method as set forth in claim 23, wherein said oil emulsion comprises both oil and water soluble emulsifiers, said emulsifiers, in admixture, having an HLB of about 3 to 15.
Claim 28
Dependent Claim
29 words
A method as set forth in claim 24, wherein said bacteria count is within the range of approximately 1×10 3 to 1×10 9 CFU per cc of said emulsion.
References Cited (Backward Citations)
US1758937A
Cited
US4395394A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-26 | 1989-11-25 | 1990-05-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-26 | 1993-11-25 | 1994-05-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-26 | 1997-11-25 | 1998-05-27 | $7700.00 | Window Closed / Lapsed |