US4590170A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing microcapsule reagents for immunological response
Assignee: Fujifilm Holdings Corp
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Filing: 1981-07-08
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Granted: 1986-05-20
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Expires: 2001-07-08
Abstract
Abstract A process for producing a microcapsule diagnostic material utilizing passive agglutination which comprises binding an antigen or antibody onto a microcapsule wall negatively charged, in an acidic solution is provided. The microcapsule diagnostic material thus produced has an extremely high sensitivity in agglutination without causing any non-specific agglutination.
Inventors
- 👤 Yutaka Akiyoshi
- 👤 Shinzo Kobayashi
- 👤 Fujio Kakimi
- 👤 Hiroharu Matsukawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:04
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S436/00; Y10S436/829; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984; Y10T428/2985; Y10T428/2987
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
63 words
A process for producing a microcapsule reagent for passive agglutination which comprises binding an antigen or antibody onto an exterior wall surface of a microcapsule, which microcapsule comprises a wall material having encapsulated therein an oily substance as a core and which microcapsule has an average particle size of 0.1 to 30 microns and which microcapsule is negatively charged in an acidic solution.
Claim 2
Dependent Claim
63 words
A process for producing a microcapsule reagent for passive agglutination which comprises binding an antigen or antibody onto an exterior wall surface of a microcapsule, which microcapsule comprises a wall material having encapsulated therein an oily substance as a core and which microcapsule has an average particle size of 0.1 to 30 microns and which microcapsule is negatively charged in an acidic solution.
Claim 3
Dependent Claim
19 words
The process of claim 1 wherein said microcapsule negatively charged possesses a zeta potential more negative than -20 mV.
Claim 4
Dependent Claim
15 words
The process of claim 1 wherein said binding is effected through a cross linking agent.
Claim 5
Dependent Claim
33 words
The process of claim 3 wherein said cross linking agent is selected from the group consisting of a dialdehyde, a water-soluble carbodiimide, an isoxazolium salt, an imide ester, a diisocyanate and a halonitrobenzene.
Claim 6
Dependent Claim
16 words
The process of claim 1 wherein said acidic solution has pH of from 3.0 to 5.5.
Claim 7
Dependent Claim
35 words
The process of a claim 1 wherein said wall surface comprises a member selected from the group consisting of polyvinyl sulfates, polyvinyl sulfonates, polystyrene sulfonates, polyacrylic acids, maleic anhydride copolymers and alkali metal salts thereof.
Claim 8
Dependent Claim
33 words
The process of claim 1 wherein the amount of said wall is in the range of 2 to 50 weight percent based on the weight of the oily substance which comprises the core.
Claim 9
Dependent Claim
61 words
The process of claim 1 wherein said microcapsules have a size of 0.5-10μ, exhibit a zeta potential of -40 to -60 mV and wherein said binding of said antigen or antibody is at a pH of 4 to 5.5 and wherein the core substance is completely enclosed by the capsule walls and hence does not directly affect the antigen or antibody.
Claim 10
Dependent Claim
72 words
A process for producing a microcapsule reagent passive agglutination which comprises binding an antigen or antibody onto an exterior wall surface of a microcapsule, which microcapsule comprises a wall material having encapsulated therein an oily substance as a core and which microcapsule has a size from 0.1 to 30 microns, said microcapsule being prepared in the presence of a high molecular weight compound containing an anionic group(s) in a side chain thereof.
Claim 11
Dependent Claim
72 words
A process for producing a microcapsule reagent passive agglutination which comprises binding an antigen or antibody onto an exterior wall surface of a microcapsule, which microcapsule comprises a wall material having encapsulated therein an oily substance as a core and which microcapsule has a size from 0.1 to 30 microns, said microcapsule being prepared in the presence of a high molecular weight compound containing an anionic group(s) in a side chain thereof.
Claim 12
Dependent Claim
40 words
The process of claim 9 wherein said anionic group(s) is/are selected from the group consisting of a sulfonic acid group, a sulfuric acid group, a carboxylic acid group, a maleic acid group, and a group derived from a salt thereof.
Claim 13
Dependent Claim
22 words
The process of claim 9 wherein said high molecular weight compound has a molecular weight of from about 10,000 to about 1,000,000.
Claim 14
Dependent Claim
61 words
The process of claim 9 wherein said microcapsules have a size of 0.5-10μ, exhibit a zeta potential of -40 to -60 mV and wherein said binding of said antigen or antibody is at a pH of 4 to 5.5 and wherein the core substance is completely enclosed by the capsule walls and hence does not directly affect the antigen or antibody.
Claim 15
Dependent Claim
43 words
The process of claim 1 or claim 9 wherein said wall material is selected from the group consisting of a protein, a polyamino acid, a polyacryl amide, a polyamide, a polyurethane, a polyurea, a melamine, cellulose and derivatives thereof, gum arabic and starch.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-19 | 1989-11-18 | 1990-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-19 | 1993-11-18 | 1994-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-19 | 1997-11-18 | 1998-05-20 | $7700.00 | Window Closed / Lapsed |