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US4589881A Expired / Lapsed US ⏱️ 0.0 years remaining

Polypeptide

Assignee: Sanford Burnham Prebys Medical Discovery Institute
•
Filing: 1983-07-28
•
Granted: 1986-05-20
•
Expires: 2003-07-28
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Abstract

Abstract A polypeptide having the cell-attaching activity of fibronectin. The polypeptide has 108 amino acid residues and the formula: H-Ile-Gly-Gln-Gln-Ser-Thr-Val-Ser-Asp-Val-Pro-Arg-Asp-Leu-Glu-Val-Val-Ala-Ala-Thr-Pro-Thr-Ser-Leu-Leu-Ile-Ser-Trp-Asp-Ala-Pro-Ala-Val-Thr-Val-Arg-Tyr-Tyr-Arg-Ile-Thr-Tyr-Gly-Glu-Thr-Gly-Gly-Asn-Ser-Pro-Val-Gln-Glu-Phe-Thr-Val-Pro-Gly-Ser-Lys-Ser-Thr-Ala-T r-Ile-Ser-Gly-Leu-Lys-Pro-Gly-Val-Asp-Tyr-Thr-Ile-Thr-Val-Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-OH. The polypeptide or a biologically active fragment thereof, such as H-Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-OH can be employed in the preparation of substrata designed for the attachment of cells thereto. A Cys-residue may optionally be attached at the C-terminus. It can be linked to the surface of a prosthetic device to particularly attract endothelial cells and fibroblastic cells.

Inventors

  • 👤 Michael D. Pierschbacher
  • 👤 Erkki I. Ruoslahti

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 14:58
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815

Claims Analysis

Total Claims: 14
Claim 1
Independent Claim 12 words
The biologically active amino acid polymer consisting essentially of the polypeptide: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 2
Dependent Claim 12 words
The biologically active amino acid polymer consisting essentially of the polypeptide: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 3
Dependent Claim 31 words
A composition of matter consisting essentially of an amino acid polymer which is biologically active, exhibits cell attachment activity and is characterized as a substantially isolated polypeptide having the formula: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 4
Dependent Claim 31 words
A composition of matter consisting essentially of an amino acid polymer which is biologically active, exhibits cell attachment activity and is characterized as a substantially isolated polypeptide having the formula: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 5
Dependent Claim 25 words
A prosthetic device having a biologically active surface which exhibits cell attachment activity, said surface having linked thereto a biologically active amino acid polymer: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 6
Dependent Claim 25 words
A prosthetic device having a biologically active surface which exhibits cell attachment activity, said surface having linked thereto a biologically active amino acid polymer: Tyr-Ala-Val-Thr-Gly-Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro-Ile-Ser-Ile-Asn-Tyr-Arg-Thr-Glu-Ile-Asp-Lys-Pro-Ser-Gln-Met-R-OH.
Claim 7
Dependent Claim 11 words
A polypeptide in accordance with claim 1 wherein R is Cys.
Claim 8
Dependent Claim 11 words
A polypeptide in accordance with claim 1 wherein R is des-R.
Claim 9
Dependent Claim 10 words
A polypeptide in accordance with claim 1 coupled to collagen.
Claim 10
Dependent Claim 12 words
A prosthetic device in accordance with claim 3 wherein R is Cys.
Claim 11
Dependent Claim 12 words
A prosthetic device in accordance with claim 3 wherein R is des-R.
Claim 12
Dependent Claim 18 words
A prosthetic device in accordance with claim 3 wherein said surface constitutes a portion of a vascular graft.
Claim 13
Dependent Claim 17 words
A prosthetic device in accordance with claim 3 wherein said surface is made of asynthetic resin fiber.
Claim 14
Dependent Claim 18 words
A prosthetic device in accordance with claim 3 wherein said surface constitutes a portion of a percutaneous device.

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1989-05-19 1989-11-18 1990-05-20 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1993-05-19 1993-11-18 1994-05-20 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1997-05-19 1997-11-18 1998-05-20 $7700.00 Window Closed / Lapsed

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