US4588682A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Binding nucleic acid to a support
Assignee: Genzyme Corp
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Filing: 1982-12-13
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Granted: 1986-05-13
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Expires: 2002-12-13
Abstract
Abstract Method of binding nucleic acid to a nucleic acid-binding support comprising depositing the nucleic acid onto the support and then contacting the nucleic acid and the support with a liquid binding solution which is compatible with the support and which contains an organic solvent which is capable of binding the DNA to the support, for a period of time sufficient to effect the binding.
Inventors
- 👤 Suzanne Groet
- 👤 Jonathan Ostman
- 👤 Robert Wydro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 10:32
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
73 words
A method of binding nucleic acid to a nucleic acid-binding support comprising depositing without fixing, said nucleic acid onto said support following denaturation of said nucleic acid, and then contacting said nucleic acid and said support with a liquid binding solution which is compatible with said support and which contains an organic solvent which is capable of binding said DNA to said support, for a period of time sufficient to effect said binding.
Claim 2
Independent Claim
73 words
A method of binding nucleic acid to a nucleic acid-binding support comprising depositing without fixing, said nucleic acid onto said support following denaturation of said nucleic acid, and then contacting said nucleic acid and said support with a liquid binding solution which is compatible with said support and which contains an organic solvent which is capable of binding said DNA to said support, for a period of time sufficient to effect said binding.
Claim 3
Dependent Claim
35 words
The method of claim 1 wherein said nucleic acid is included in a sample to be assayed by hybridization for a predetermined type of nucleic acid, said sample being deposited on said nucleic acid-binding support.
Claim 4
Dependent Claim
29 words
The method of claim 2 wherein said nucleic acid is DNA and, prior to said binding, said DNA is denatured and then neutralized for use in said hybridization assay.
Claim 5
Dependent Claim
29 words
The method of claim 1 wherein said nucleic acid and said support, after said depositing, are permitted to air-dry prior to being contacted with said nucleic acid binding solution.
Claim 6
Dependent Claim
28 words
The method of claim 2 wherein said contacting with said nucleic acid binding solution does not alter said nucleic acid in a manner which interferes with said hybridization.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein said organic solvent contains fewer than 20 carbon atoms.
Claim 8
Dependent Claim
20 words
The method of claim 1 wherein said nucleic acid binding solution is made up substantially entirely of said organic solvent.
Claim 9
Dependent Claim
20 words
The method of claim 6 wherein said organic solvent is an alcohol, an ether, an aromatic compound, or a ketone.
Claim 10
Dependent Claim
34 words
The method of claim 1 wherein said nucleic acid binding solution contains a mixture of more than one organic solvent, said mixture being capable of binding said nucleic acid to said nucleic acid-binding support.
Claim 11
Dependent Claim
16 words
The method of claim 9 wherein each said organic solvent contains fewer than 20 carbon atoms.
Claim 12
Dependent Claim
22 words
The method of claim 9 wherein each said organic solvent., independently, is an alcohol, an ether, an aromatic compound, or a ketone.
Claim 13
Dependent Claim
24 words
The method of claim 8 wherein said organic solvent is ethanol, methanol, sec-butyl alcohol, iso-amyl alcohol, isopropyl alcohol, isobutyl alcohol, ethyl ether, or toluene.
Claim 14
Dependent Claim
26 words
The method of claim 11 wherein each said organic solvent, independently, is ethanol, methanol, sec-butyl alcohol, iso-amyl alcohol, isopropyl alcohol, isobutyl alcohol, ethyl ether, or toluene.
Claim 15
Dependent Claim
12 words
The method of claim 8 wherein said organic solvent is sec-butyl alcohol.
Claim 16
Dependent Claim
14 words
The method of claim 13 wherein one of said organic solvents is sec-butyl alcohol.
Claim 17
Dependent Claim
17 words
The method of claim 1 wherein said period of time is between 1 second and 10 minutes.
Claim 18
Dependent Claim
14 words
The method of claim 16 wherein said period of time is about 5 minutes.
Claim 19
Dependent Claim
19 words
The method of claim 1 wherein, following said contacting, said support and said nucleic acid are permitted to air-dry.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-12 | 1989-11-11 | 1990-05-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-12 | 1993-11-11 | 1994-05-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-12 | 1997-11-11 | 1998-05-13 | $7700.00 | Window Closed / Lapsed |