US4587223A
Expired / Lapsed
US
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Method for quantitative determination of a biospecific affinity reaction
Assignee: Wallac Oy
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Filing: 1983-08-25
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Granted: 1986-05-06
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Expires: 2003-08-25
Abstract
Abstract Method for quantitative determination of a biospecific affinity reaction in which an immunochemical compound labelled with a lanthanide chelate is used which can be detected by means of time-resolved fluorescence in which the amount of labelled compound present in the biospecific affinity reaction can be measured without the separation of free and biospecifically bound labelled compound having to be carried out.
Inventors
- 👤 Erkki Soini
- 👤 Ilkka Hemmila
- 👤 Timo Lovgren
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 15:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N2458/00; G01N2458/40; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
69 words
Method for quantitative determination of a homogeneous immunological reaction, comprising reacting a labelled immunochemical compound with its immunochemical counterpart, labelling the immunochemical compound with a europium or terbium chelate, wherein the improvement comprises measuring the intensity and/or the half-life of the fluorescence of the chelate by excitation of the chelate with a light pulse, and detection of the fluorescence after a predetermined time has elapsed from that light pulse.
Claim 2
Dependent Claim
69 words
Method for quantitative determination of a homogeneous immunological reaction, comprising reacting a labelled immunochemical compound with its immunochemical counterpart, labelling the immunochemical compound with a europium or terbium chelate, wherein the improvement comprises measuring the intensity and/or the half-life of the fluorescence of the chelate by excitation of the chelate with a light pulse, and detection of the fluorescence after a predetermined time has elapsed from that light pulse.
Claim 3
Dependent Claim
14 words
Method according to claim 1, wherein the chelate contains a β-diketone and/or trioctylphosphine oxide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-05 | 1989-11-04 | 1990-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-05 | 1993-11-04 | 1994-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-05 | 1997-11-04 | 1998-05-06 | $7700.00 | Window Closed / Lapsed |