US4585735A
Expired / Lapsed
US
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Prolonged storage of red blood cells
Assignee: American Red Cross
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Filing: 1984-07-19
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Granted: 1986-04-29
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Expires: 2004-07-19
Abstract
Abstract The present invention discloses a hypotonic suspension medium and a method for prolonged storage of red blood cells at about 4 DEG C. The suspension medium comprises about 80-150 mM glucose, 30-80 mM mannitol, 6-90 mM potassium citrate, 1-5 mM adenine and 10-200 mM ammonium chloride or acetate in an aqueous solution having a pH of about 6.8 to 7.2 and an osmolarity of about 180-220 milli-osmolar. The suspension medium and the method of the present invention allow viable storage of red blood cells at about 4 DEG C. up to 125 days or more.
Inventors
- 👤 Harold T. Meryman
- 👤 Marne Hornblower
- 👤 Ralph Syring
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:51
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/18
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
78 words
A suspension medium for prolonged storage of red blood cells at about 4° C. consisting essentially of an aqueous solution containing about 80-150 mM glucose, 30-80 mM mannitol, 6-90 mM potassium citrate, 1-5 mM adenine and 40-95 mM of a phosphate salt, said solution having an osmolarity of about 180-220 milli-osmolar and having 10-200 mM of a penetrating salt, the pH of said solution being about 6.8 to 7.2 wherein said red blood cells can be viably stored.
Claim 2
Dependent Claim
78 words
A suspension medium for prolonged storage of red blood cells at about 4° C. consisting essentially of an aqueous solution containing about 80-150 mM glucose, 30-80 mM mannitol, 6-90 mM potassium citrate, 1-5 mM adenine and 40-95 mM of a phosphate salt, said solution having an osmolarity of about 180-220 milli-osmolar and having 10-200 mM of a penetrating salt, the pH of said solution being about 6.8 to 7.2 wherein said red blood cells can be viably stored.
Claim 3
Independent Claim
83 words
A method of preserving red blood cells for extended period of time at about 4° C. comprising suspending said cells in an aqueous solution consisting essentially of about 80-150 mM glucose, 30-80 mM mannitol, 6-90 mM potassium citrate, 1-5 mM adenine and 40-95 mM of a phosphate salt, said solution having an osmolarity of about 180-220 milli-osmolar and having 10-200 mM of a penetrating salt, the pH of said solution being about 6.8 to 7.2 allowing viable storage of said red blood cells.
Claim 4
Independent Claim
83 words
A method of preserving red blood cells for extended period of time at about 4° C. comprising suspending said cells in an aqueous solution consisting essentially of about 80-150 mM glucose, 30-80 mM mannitol, 6-90 mM potassium citrate, 1-5 mM adenine and 40-95 mM of a phosphate salt, said solution having an osmolarity of about 180-220 milli-osmolar and having 10-200 mM of a penetrating salt, the pH of said solution being about 6.8 to 7.2 allowing viable storage of said red blood cells.
Claim 5
Dependent Claim
22 words
The suspension medium of claim 1 wherein said penetrating salt is selected from the group consisting of ammonium chloride and ammonium acetate.
Claim 6
Dependent Claim
28 words
The suspension medium of claim 3 containing about 110 mM glucose, 55 mM mannitol, 7.9 mM potassium citrate, 2 mM adenine and 50 mM ammonium chloride or acetate.
Claim 7
Dependent Claim
30 words
The suspension medium of claim 4 wherein the pH is maintained at a value of about 7.0 by adjusting the pH with suitable amounts of mono or dibasic potassium phosphate.
Claim 8
Dependent Claim
13 words
The suspension medium of claim 5 wherein the osmolarity is about 200 mOsm.
Claim 9
Dependent Claim
21 words
The method of claim 2 wherein said penetrating salt is selected from the group consisting of ammonium chloride and ammonium acetate.
Claim 10
Dependent Claim
27 words
The method of claim 7 containing about 110 mM glucose, 55 mM mannitol, 7.9 mM potassium citrate, 2 mM adenine and 50 mM ammonium chloride or acetate.
Claim 11
Dependent Claim
29 words
The method of claim 8 wherein the pH is maintained at a value of about 7.0 by adjusting the pH with suitable amounts of mono or dibasic potassium phosphate.
Claim 12
Dependent Claim
12 words
The method of claim 9 wherein the osmolarity is about 200 mOsm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-28 | 1989-10-28 | 1990-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-28 | 1993-10-28 | 1994-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-28 | 1997-10-28 | 1998-04-29 | $7700.00 | Window Closed / Lapsed |