US4584268A
Expired / Lapsed
US
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Method and compositions for carcinoma diagnosis
Assignee: Individual
•
Filing: 1984-02-24
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Granted: 1986-04-22
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Expires: 2004-02-24
Abstract
Abstract Methods and compositions are provided for detecting the presence of carcinomas in a mammalian host by measuring the level of normal surface antigens specific for a differentiated cell in the serum of the host as compared to the normal level of such antigen. The method finds particular use in detecting residual carcinomas after therapy or in detecting the recurrence of neoplastic tissue, and assigning a tissue of origin to the neoplastic tissue.
Inventors
- 👤 Roberto L. Ceriani
- 👤 Jerry A. Peterson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57515; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/811; Y10S436/813; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
113 words
An assay method for detecting the presence of neoplastic mammary epithelial tissue from a solid tumor or metastasis of a mammary organ of a mammalian host which comprises: combining a serum or plasma sample of said host with antibodies specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen present in said serum or plasma binds to said antibodies to form an antigen-antibody complex; determining the amount of said complex; and comparing the amount of said complex in said serum or plasma with the amount of complex in a mammalian host free of neoplastic tissue of said mammary organ.
Claim 2
Dependent Claim
113 words
An assay method for detecting the presence of neoplastic mammary epithelial tissue from a solid tumor or metastasis of a mammary organ of a mammalian host which comprises: combining a serum or plasma sample of said host with antibodies specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen present in said serum or plasma binds to said antibodies to form an antigen-antibody complex; determining the amount of said complex; and comparing the amount of said complex in said serum or plasma with the amount of complex in a mammalian host free of neoplastic tissue of said mammary organ.
Claim 3
Dependent Claim
19 words
A method according to claim 1, where a competitive or pseudo-competitive protein binding assay is employed for said assay.
Claim 4
Dependent Claim
12 words
A method according to claim 2, wherein said assay is a radioimmunoassay.
Claim 5
Dependent Claim
13 words
A method according to claim 2, wherein said assay is an enzyme immunoassay,
Claim 6
Dependent Claim
14 words
A method according to claim 4, wherein said enzyme immunoassay is a heterogeneous immunoassay.
Claim 7
Dependent Claim
14 words
A method according to claim 4, wherein said enzyme immunoassay is a homogeneous immunoassay.
Claim 8
Dependent Claim
13 words
A method according to claim 2, wherein said assay is a fluorescent assay.
Claim 9
Independent Claim
137 words
A method for detecting the presence of neoplastic tissue from a solid tumor or metastiasis of a mammary organ of a mammalian host which comprises: combining serum from said host with antibodies bound to a support, wherein said antibodies are specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen present in said serum binds to said antibodies to form an antigen-antibody complex; adding labelled antibodies specific for said surface antigen which bind to said surface antigen in said antigen-antibody complex to form a sandwich; separating said support from any liquid; and determining the amount of label bound to said support as compared to the amount of label bound to said support in the presence of a known amount of said surface antigen.
Claim 10
Independent Claim
137 words
A method for detecting the presence of neoplastic tissue from a solid tumor or metastiasis of a mammary organ of a mammalian host which comprises: combining serum from said host with antibodies bound to a support, wherein said antibodies are specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen present in said serum binds to said antibodies to form an antigen-antibody complex; adding labelled antibodies specific for said surface antigen which bind to said surface antigen in said antigen-antibody complex to form a sandwich; separating said support from any liquid; and determining the amount of label bound to said support as compared to the amount of label bound to said support in the presence of a known amount of said surface antigen.
Claim 11
Dependent Claim
12 words
A method according to claim 8, wherein said label is a radionuclide.
Claim 12
Independent Claim
16 words
A method according to any of claims 8 or 9, wherein said organ is the breast.
Claim 13
Independent Claim
101 words
A method for detecting the presence of neoplastic tissue from a solid tumor or metastases of a mammary organ of a mammalian host, which comprises: combining serum from said host with antibodies bound to a support, wherein said antibodies are specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen in said serum binds to said antibodies to form an antigen-antibody complex; labelling said complex with a label; releasing labelled surface antigen from said labelled complex; and characterizing said labelled antigen in comparison to known surface antigen.
Claim 14
Independent Claim
101 words
A method for detecting the presence of neoplastic tissue from a solid tumor or metastases of a mammary organ of a mammalian host, which comprises: combining serum from said host with antibodies bound to a support, wherein said antibodies are specific for at least one surface antigen specific for a normal differentiated epithelial cell of said mammary organ, wherein any of said surface antigen in said serum binds to said antibodies to form an antigen-antibody complex; labelling said complex with a label; releasing labelled surface antigen from said labelled complex; and characterizing said labelled antigen in comparison to known surface antigen.
Claim 15
Independent Claim
50 words
A method for determining the presence of neoplastic tissue from a solid tumor or metastasis of a mammary organ of a mammalian host, which comprises: monitoring the level of at least one surface antigen specific for normal differentiated epithelial cells of said mammary organ in the serum of said host.
Claim 16
Independent Claim
50 words
A method for determining the presence of neoplastic tissue from a solid tumor or metastasis of a mammary organ of a mammalian host, which comprises: monitoring the level of at least one surface antigen specific for normal differentiated epithelial cells of said mammary organ in the serum of said host.
Claim 17
Dependent Claim
55 words
A kit for monitoring the level of a surface antigen specific for a normal differentiated epithelial cell of a mammary organ of a mammalian host which comprises: in a container lyophilized antibodies specific for said surface antigen formulated with sufficient amounts of buffer, inert protein and preservatives for use with a blood or plasma sample.
Claim 18
Dependent Claim
55 words
A kit for monitoring the level of a surface antigen specific for a normal differentiated epithelial cell of a mammary organ of a mammalian host which comprises: in a container lyophilized antibodies specific for said surface antigen formulated with sufficient amounts of buffer, inert protein and preservatives for use with a blood or plasma sample.
Claim 19
Dependent Claim
15 words
A kit according to claim 13, wherein said antibodies are bound to a solid support.
Claim 20
Dependent Claim
15 words
A kit according to any of claim 13 or 14, wherein said antibodies are labelled.
Claim 21
Dependent Claim
12 words
A kit according to claim 15, wherein the label is a radionuclide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-21 | 1989-10-21 | 1990-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-21 | 1993-10-21 | 1994-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-21 | 1997-10-21 | 1998-04-22 | $7700.00 | Window Closed / Lapsed |