Method of separation of nucleotides and nucleosides from each other

Claim 1

A method for the separation of nucleotides and nucleosides from each other, which comprises contacting a solution containing nucleotides and nucleosides, after being adjusted to a pH of not more than 7, with activated carbon to absorb the nucleosides and nucleotides and then eluting the nucleotides with an aqueous solution of an alkali metal hydroxide, the said nucleotides being from the group of inosine-5'-monophosphate, guanosine-5'-monophosphate, adenosine-5'-monophosphate, xanthosine-5'-monophosphate, cytidine-5'-monophosphate, uridine-5'-monophosphate, deoxyadenosine-5'-monophosphate, deoxycytidine-5'-monophosphate, deoxyuridine-5'-monophosphate, inosine-2'(3'),5'-diphosphate, inosine-5'-diphosphate, guanosine-2'(3'),5'-diphosphate, guanosine-5'-diphosphate, adenosine-2'(3'),5'-diphosphate, adenosine-5'-diphosphate, cytidine-5'-diphosphate, uridine-5'-diphosphate, deoxyadenosine-5'-diphosphate, deoxycytidine-5'-diphosphate, deoxyuridine-5'-diphosphate, adenosine-5'-triphosphate, guanosine-5'-triphosphate, deoxyadenosine-5'-triphosphate, deoxyguanosine-5'-triphosphate, deoxyctidine-5'-triphosphate, deoxyuridine-5'-triphosphate, inosine-2'-monophosphate, guanosine-3'-monophosphate, adenosine-3',5'-cyclic monophosphate, and xanthine-2',3'-cyclic monophosphate, the said nucleosides being inosine, guanosine, adenosine, xanthosine, cytidine, uridine, thymidine and orotidine and a deoxyribose homolog of said compounds and the said activated carbon having pore characteristics such that not less than 0.6 cc/g of the total pore volume is occupied by pores having a diameter of not more than 15μ, not less than 0.4 cc/g of the total pore volume is occupied by pores having a diameter of not more than 300 Å and not less than 17 Å of an average pore diameter for pores having a diameter of not more than 300 Å and, following the nucleotide elution, eluting the nucleosides with an aqueous solution containing an alkali metal hydroxide and a lower alcohol.

Claim 2

A method for the separation of nucleotides and nucleosides from each other, which comprises contacting a solution containing nucleotides and nucleosides, after being adjusted to a pH of not more than 7, with activated carbon to absorb the nucleosides and nucleotides and then eluting the nucleotides with an aqueous solution of an alkali metal hydroxide, the said nucleotides being from the group of inosine-5'-monophosphate, guanosine-5'-monophosphate, adenosine-5'-monophosphate, xanthosine-5'-monophosphate, cytidine-5'-monophosphate, uridine-5'-monophosphate, deoxyadenosine-5'-monophosphate, deoxycytidine-5'-monophosphate, deoxyuridine-5'-monophosphate, inosine-2'(3'),5'-diphosphate, inosine-5'-diphosphate, guanosine-2'(3'),5'-diphosphate, guanosine-5'-diphosphate, adenosine-2'(3'),5'-diphosphate, adenosine-5'-diphosphate, cytidine-5'-diphosphate, uridine-5'-diphosphate, deoxyadenosine-5'-diphosphate, deoxycytidine-5'-diphosphate, deoxyuridine-5'-diphosphate, adenosine-5'-triphosphate, guanosine-5'-triphosphate, deoxyadenosine-5'-triphosphate, deoxyguanosine-5'-triphosphate, deoxyctidine-5'-triphosphate, deoxyuridine-5'-triphosphate, inosine-2'-monophosphate, guanosine-3'-monophosphate, adenosine-3',5'-cyclic monophosphate, and xanthine-2',3'-cyclic monophosphate, the said nucleosides being inosine, guanosine, adenosine, xanthosine, cytidine, uridine, thymidine and orotidine and a deoxyribose homolog of said compounds and the said activated carbon having pore characteristics such that not less than 0.6 cc/g of the total pore volume is occupied by pores having a diameter of not more than 15μ, not less than 0.4 cc/g of the total pore volume is occupied by pores having a diameter of not more than 300 Å and not less than 17 Å of an average pore diameter for pores having a diameter of not more than 300 Å and, following the nucleotide elution, eluting the nucleosides with an aqueous solution containing an alkali metal hydroxide and a lower alcohol.

Claim 3

A method of separation of nucleotides and 5-amino-4-imidazolecarboxyamidoriboside from each other, which comprises contacting a solution containing nucleotides and 5-amino-4-imidazolecarboxyamidoriboside, after being adjusted to a pH of not more than 7, with activated carbon to absorbe 5-amino-4-imidazolecarboxyamidoriboside and nucleotides and then eluting the nucleotides with an aqueous solution of an alkali metal hydroxide, the said nucleotides being from the group inosine-5'-monophosphate, quanosine-5'-monophosphate, adenosine-5'-monophosphate, xanthosine-5'-monophosphate, cytidine-5'-monophosphate, uridine-5'-monophosphate, deoxyadenosine-5'-monophosphate, deoxycytidine-5'-monophosphate, deoxyuridine-5'-monophosphate, inosine-2'(3'),5'-diphosphate, inosine-5'-diphosphate, guanosine-2'(3'),5'-diphosphate, guanosine-5'-diphosphate, adenosine-2'(3'),5'-diphosphate, adenosine-5'-diphosphate, cytidine-5'-diphosphate, uridine-5'-diphosphate, deoxyadenosine-5'-diphosphate, deoxycytidine-5'-diphosphate, deoxyuridine-5'-diphosphate, adenosine-5'-triphosphate, guanosine-5'-triphosphate, deoxyadenosine-5'-triphosphate, deoxyguanosine-5'-triphosphate, deoxycytidine-5'-triphosphate, deoxyuridine-5'-triphosphate, inosine-2'-monophosphate, guanosine-3'-monophosphate, adenosine-3',5'-cyclic monophosphate, and xanthine-2',3'-cyclic monophosphate, and the said activated carbon having pore characteristics such that not less than 0.6 cc/g of the total pore volume is occupied by pores having a diameter of not more than 15μ, not less than 014 cc/g of total pore volume is occupied by pores having a diameter of not more than 300 Å and not less than 17 Å of an average pore diameter for pores having a diameter of not more than 300 Å and following the nucleotide elution, eluting the 5-amino-4-imidazolecarboxyamidoriboside with an aqueous solution containing an alkali metal hydroxide and a lower alcohol.

Claim 4

A method of separation of nucleotides and 5-amino-4-imidazolecarboxyamidoriboside from each other, which comprises contacting a solution containing nucleotides and 5-amino-4-imidazolecarboxyamidoriboside, after being adjusted to a pH of not more than 7, with activated carbon to absorbe 5-amino-4-imidazolecarboxyamidoriboside and nucleotides and then eluting the nucleotides with an aqueous solution of an alkali metal hydroxide, the said nucleotides being from the group inosine-5'-monophosphate, quanosine-5'-monophosphate, adenosine-5'-monophosphate, xanthosine-5'-monophosphate, cytidine-5'-monophosphate, uridine-5'-monophosphate, deoxyadenosine-5'-monophosphate, deoxycytidine-5'-monophosphate, deoxyuridine-5'-monophosphate, inosine-2'(3'),5'-diphosphate, inosine-5'-diphosphate, guanosine-2'(3'),5'-diphosphate, guanosine-5'-diphosphate, adenosine-2'(3'),5'-diphosphate, adenosine-5'-diphosphate, cytidine-5'-diphosphate, uridine-5'-diphosphate, deoxyadenosine-5'-diphosphate, deoxycytidine-5'-diphosphate, deoxyuridine-5'-diphosphate, adenosine-5'-triphosphate, guanosine-5'-triphosphate, deoxyadenosine-5'-triphosphate, deoxyguanosine-5'-triphosphate, deoxycytidine-5'-triphosphate, deoxyuridine-5'-triphosphate, inosine-2'-monophosphate, guanosine-3'-monophosphate, adenosine-3',5'-cyclic monophosphate, and xanthine-2',3'-cyclic monophosphate, and the said activated carbon having pore characteristics such that not less than 0.6 cc/g of the total pore volume is occupied by pores having a diameter of not more than 15μ, not less than 014 cc/g of total pore volume is occupied by pores having a diameter of not more than 300 Å and not less than 17 Å of an average pore diameter for pores having a diameter of not more than 300 Å and following the nucleotide elution, eluting the 5-amino-4-imidazolecarboxyamidoriboside with an aqueous solution containing an alkali metal hydroxide and a lower alcohol.