US4572897A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carrier for immobilizing enzymes
Assignee: Novozymes AS
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Filing: 1983-10-05
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Granted: 1986-02-25
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Expires: 2003-10-05
Abstract
Abstract Immobilized enzyme granules prepared by mixing inert filler particles into an aqueous solution or dispersion of a water soluble binder, followed by granulating the mixture so as to form carrier granules. Enzyme is immobilized on the hydrophilic surfaces of the carrier granules. If not already insoluble in the medium for the intended enzymatic reaction, the binder in the granules is rendered insoluble, e.g., water insoluble. Proteins are preferred binder materials, and glutaraldehyde is the preferred reagent for bonding enzyme to the carrier granules and for water insolubilizing the binder.
Inventors
- 👤 Shmuel Amotz
- 👤 Susanne Rugh
- 👤 Erik K. Markussen
- 👤 Kurt Thomsen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 16:07
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/14
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
68 words
An immobilized enzyme granule adapted for fixed bed or fluidized bed continuous enzymatic reactions comprising: (a) a continuous phase hydrophilic binder material, (b) a discontinuous phase particulate inert filler material of particle size less than 1/5 of the least dimension of the granule, said binder and filler being insoluble in the enzymatic reaction medium, and (c) enzyme immobilized to the binder material at the surfaces of said granule.
Claim 2
Dependent Claim
68 words
An immobilized enzyme granule adapted for fixed bed or fluidized bed continuous enzymatic reactions comprising: (a) a continuous phase hydrophilic binder material, (b) a discontinuous phase particulate inert filler material of particle size less than 1/5 of the least dimension of the granule, said binder and filler being insoluble in the enzymatic reaction medium, and (c) enzyme immobilized to the binder material at the surfaces of said granule.
Claim 3
Dependent Claim
63 words
An immobilized enzyme granule adapted for employment in an aqueous medium comprising: (a) a continuous phase of hydrophilic binder material and a discontinuous phase of particulate inert filler material of particle size less than 1/5 of the least dimension of the granule, said binder and filler being water insoluble, and (b) enzyme crosslinked to the binder material at the surfaces of said granule.
Claim 4
Dependent Claim
63 words
An immobilized enzyme granule adapted for employment in an aqueous medium comprising: (a) a continuous phase of hydrophilic binder material and a discontinuous phase of particulate inert filler material of particle size less than 1/5 of the least dimension of the granule, said binder and filler being water insoluble, and (b) enzyme crosslinked to the binder material at the surfaces of said granule.
Claim 5
Dependent Claim
35 words
The immobilized enzyme granule of claim 2 further comprising a protein binder material which is water insolubilized by reaction with glutaraldehyde, and an enzyme which is crosslinked to the binder material by reaction with glutaraldehyde.
Claim 6
Dependent Claim
25 words
The enzyme granule of claim 3 further comprising a gelatine binder and diatomaceous earth and cellulose fiber filler materials, and glucose isomerase as the enzyme.
Claim 7
Dependent Claim
80 words
Method for production of immobilized enzyme granules which comprises: (a) admixing a water soluble binder dissolved or dispersed in an aqueous medium, and water insoluble inert filler particles, thereafter forming carrier granules from said mixture, then rendering the binder water insoluble and immobilizing an enzyme to the binder material at the surfaces of the carrier granules each of said filler particles having a particle size less than 1/5 of the least dimension of the granule in which it is contained.
Claim 8
Dependent Claim
80 words
Method for production of immobilized enzyme granules which comprises: (a) admixing a water soluble binder dissolved or dispersed in an aqueous medium, and water insoluble inert filler particles, thereafter forming carrier granules from said mixture, then rendering the binder water insoluble and immobilizing an enzyme to the binder material at the surfaces of the carrier granules each of said filler particles having a particle size less than 1/5 of the least dimension of the granule in which it is contained.
Claim 9
Dependent Claim
16 words
Method according to claim 5 further comprising shaping the mixture directly into granules by mechanical action.
Claim 10
Dependent Claim
58 words
Method according to claim 5 further comprising: (a) including a gellable agent dissolved in said aqueous medium, and (b) adding the aqueous mixture to a gelling medium for said agent so as to form granules, whereafter said binder is made water insoluble by reaction with a crosslinking agent followed by dissolving the gellable agent away from the granules.
Claim 11
Dependent Claim
20 words
Method according to claim 7 wherein the gellable agent is selected from the group consisting of alginate, carrageenan or chitosan.
Claim 12
Dependent Claim
23 words
Method according to claim 8 wherein the gelling medium is a solution containing a calcium, barium or potassium salt of polyphosphate or ferricyanide.
Claim 13
Dependent Claim
11 words
Method according to claim 5 wherein the binder comprises a protein.
Claim 14
Dependent Claim
17 words
Method according to claim 10 wherein the binder is insolubilized by being crosslinked through reaction with glutaraldehyde.
Claim 15
Dependent Claim
34 words
Method according to claim 5 wherein the amount of the discontinuous phase filler material forms between about 10 and about 98 weight %, in relation to the total weight of the immobilized enzyme granules.
Claim 16
Dependent Claim
50 words
Method according to claim 5 wherein the linear size of a single inert filler particle calculated as the diameter of a sphere with the same volume as the inert particle is less than 1/20 of the least dimension of the immobilized enzyme granule in which said filler particle is contained.
Claim 17
Dependent Claim
22 words
Method according to claim 5 wherein each immobilized enzyme granule is spherical or rounded, of average diameter between 0.1 and 5 mm.
Claim 18
Dependent Claim
20 words
Method according to claim 5 wherein the carrier granules are reacted with a solution containing both enzyme and crosslinking agent.
Claim 19
Dependent Claim
63 words
Method according to claim 15 further comprising introducing the carrier granules into a tower as a fluidized mass of carrier granules and then a solution containing the soluble enzyme and crosslinking agent is introduced thereinto, whereafter the granule mass is removed from the tower, whereby the enzyme crosslinks to the binder at the surface of each granule and the binder becomes water insoluble.
Claim 20
Dependent Claim
67 words
Method according to claim 5 further comprising introducing the formed carrier granules into a tower as a fluidized mass of carrier granules, introducing a solution of soluble enzyme into said fluidized mass whereafter the granule mass is removed from the tower and then treated with crosslinking agent, whereby the enzyme becomes crosslinked to the binder at the surface of each granule and the binder becomes water insoluble.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-02-24 | 1989-08-26 | 1990-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-24 | 1993-08-26 | 1994-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-24 | 1997-08-26 | 1998-02-25 | $7700.00 | Window Closed / Lapsed |