US4569913A
Expired / Lapsed
US
⏱️ 0.0 years remaining
P. obtusus pyranose-2-oxidase preparation
Assignee: Cetus Oncology Corp
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Filing: 1984-02-24
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Granted: 1986-02-11
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Expires: 2004-02-24
Abstract
Abstract A substantially pure pyranose-2-oxidase preparation obtained from P. obtusus, and preferably from a high-producer strain thereof. The preparation is substantially free of glucosone-utilizing enzyme contaminants having measurable activity at a pH between about 4.4 and 7.0.
Inventors
- 👤 Kirston E. Koths
- 👤 Robert F. Halenbeck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 20:21
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
38 words
A substantially pure pyranose-2-oxidase preparation which is substantially free of glucosone-utilizing enzyme contaminants, including pyranosone dehydratase activity which have measurable activity at a selected pH between about 4.4 and 7.0, prepared from mycelia of a P obtusus culture.
Claim 2
Dependent Claim
38 words
A substantially pure pyranose-2-oxidase preparation which is substantially free of glucosone-utilizing enzyme contaminants, including pyranosone dehydratase activity which have measurable activity at a selected pH between about 4.4 and 7.0, prepared from mycelia of a P obtusus culture.
Claim 3
Dependent Claim
73 words
The preparation of claim 1 having pyranose-2-oxidase enzyme characteristics of a native tetrameric molecular weight of about 290,000 daltons; a native subunit molecular weight of about 69,000 daltons, a sedimentation coefficient of about 11.4, covalently bound flavin, absorption maxima at 276, 357, and 456 nm, relative activities toward glucose, sorbose, xylose and delta-gluconolactone substrates of approximately 100: 80: 48: 12, respectively, at 1% (w/v) substrate concentrations, and a pH optimum of about 5.0.
Claim 4
Dependent Claim
24 words
The preparation of claim 2, having the additional characteristics of having partially proteolyzed subunits with molecular weights of about 65,000, 34,000 and 30,000 daltons.
Claim 5
Dependent Claim
16 words
The preparation of claim 1, wherein the enzyme is purified in the presence of protease inhibitors.
Claim 6
Dependent Claim
62 words
The preparation of claim 1, wherein the P. obtusus cells from which the enzyme is isolated are from a culture grown from cells selected from a population of mutagenized cells according to their ability to produce a culture with a level of glucose-2-oxidase activity, in a broken-cell glucosone assay, which is at least two times that produced by cultures of unmutagenized cells.
Claim 7
Dependent Claim
30 words
The enzyme of claim 5, wherein the culture of selected P. obtusus cells has the characteristics of a culture of P. obtusus strain AU124, deposited at NRRL under No. 15595.
References Cited (Backward Citations)
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Cited
US4351902A
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US4321324A
Cited
US4321323A
Cited
US4423149A
Cited
US4442207A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-02-10 | 1989-08-12 | 1990-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-10 | 1993-08-12 | 1994-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-10 | 1997-08-12 | 1998-02-11 | $7700.00 | Window Closed / Lapsed |