US4569908A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Multiclass hybrid interferons
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1983-02-03
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Granted: 1986-02-11
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Expires: 2003-02-03
Abstract
Abstract New multiclass hybrid interferon polypeptides, their corresponding encoding recombinant DNA molecules and transformed hosts which produce the new interferons are described. The amino acid sequences of these hybrids include at least two different subsequences, one of which has substantial homology with a portion of a first class of interferon (e.g., HuIFN-α) and the other which has substantial homology with a portion of a second class of interferon e.g., HuIFN-β). Data indicates the interferon activity of α-β hybrids may be substantially restricted to either cell growth regulatory activity or antiviral activity.
Inventors
- 👤 David F. Mark
- 👤 Abla A. Creasey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/811; Y10S930/00; Y10S930/01; Y10S930/14; Y10S930/142
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
86 words
DNA having a nucleotide sequence that encodes a multiclass hybrid interferon polypeptide having an amino acid sequence composed of at least two distinct amino acid subsequences one of which subsequences corresponds in amino acid identity, sequence, and number to a portion of a first interferon and the other of which corresponds in amino acid identity, sequence, and number to a portion of a second interferon of a different interferon class from the first interferon, said interferon classes selected from the group consisting of α-interferon and β-interferon.
Claim 2
Dependent Claim
86 words
DNA having a nucleotide sequence that encodes a multiclass hybrid interferon polypeptide having an amino acid sequence composed of at least two distinct amino acid subsequences one of which subsequences corresponds in amino acid identity, sequence, and number to a portion of a first interferon and the other of which corresponds in amino acid identity, sequence, and number to a portion of a second interferon of a different interferon class from the first interferon, said interferon classes selected from the group consisting of α-interferon and β-interferon.
Claim 3
Dependent Claim
19 words
The DNA of claim 1 wherein the amino acid sequence is comprised only of two distinct amino acid subsequences.
Claim 4
Dependent Claim
35 words
The DNA of claim 2 wherein the portion of the first interferon is the amino terminal end of an α-interferon and the portion of the second interferon is the carboxy terminal end of a β-interferon.
Claim 5
Dependent Claim
31 words
The DNA of claim 3 wherein the amino terminal portion comprises the amino acid sequence 1-73 of HuIFN-α1 and the carboxy terminal portion comprises the amino acid sequence 74-166 of HuIFN-β1.
Claim 6
Dependent Claim
31 words
The DNA of claim 3 wherein the amino terminal portion comprises the amino acid sequence 1-41 of HuIFN-α61A and the carboxy terminal portion comprises the amino acid sequence 47-166 of HuIFN-β1.
Claim 7
Dependent Claim
35 words
The DNA of claim 2 wherein the portion of the first interferon is the amino terminal end of a β-interferon and the portion of the second interferon is the carboxy terminal end of an α-inteferon.
Claim 8
Dependent Claim
31 words
The DNA of claim 6 wherein the amino terminal end comprises the amino acid sequence 1-73 of HuIFN-β1 and the carboxy terminal end comprises the amino acid sequence 74-167 of HuIFN-α1.
Claim 9
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 1 inserted therein.
Claim 10
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 2 inserted therein.
Claim 11
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 3 inserted therein.
Claim 12
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 4 inserted therein.
Claim 13
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 5 inserted therein.
Claim 14
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 6 inserted therein.
Claim 15
Dependent Claim
11 words
A cloning vehicle having the DNA of claim 7 inserted therein.
Claim 16
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 8.
Claim 17
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 9.
Claim 18
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 10.
Claim 19
Dependent Claim
15 words
A host microorgansim or cell that is transformed with the cloning vehicle of claim 11.
Claim 20
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 12.
Claim 21
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 15 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 22
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 13.
Claim 23
Dependent Claim
15 words
A host microorganism or cell that is transformed with the cloning vehicle of claim 14.
Claim 24
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 16 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 25
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 17 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 26
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 18 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 27
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 19 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 28
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 21 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
Claim 29
Dependent Claim
35 words
A process for producing a multiclass hybrid interferon polypeptide comprising cultivating the host of claim 22 under conditions suitable for accumulation of the multiclass hybrid interferon polypeptide and collecting said polypeptide from the resulting culture.
References Cited (Backward Citations)
US4414150A
Cited
US4456748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-02-10 | 1989-08-12 | 1990-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-02-10 | 1993-08-12 | 1994-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-02-10 | 1997-08-12 | 1998-02-11 | $7700.00 | Window Closed / Lapsed |