US4565687A
Expired / Lapsed
US
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Monoclonal antibodies specific for the unbound β subunit of human chorionic gonadotropin
Assignee: University of Michigan (System)
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Filing: 1981-11-16
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Granted: 1986-01-21
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Expires: 2001-11-16
Abstract
Abstract Hybrid-myeloma cells which produce monoclonal antibody specific to the beta subunit of human chorionic gonadotropin and methods of use of the monoclonal antibody.
Inventors
- 👤 Mohammad B. Khazaeli
- 👤 William H. Beierwaltes
- 👤 Barry G. England
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 06:18
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K51/10; A61K51/1024; A61K51/1045; A61K51/1048; C07K16/28; C07K16/30; C07K16/3007; A61K2121/00; A61K2123/00; A61K38/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
47 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Claim 2
Dependent Claim
47 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Claim 3
Dependent Claim
48 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a myeloma murine cell line that produces and secretes monoclonal antibodies of the IgG class that substantially specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Claim 4
Dependent Claim
48 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a myeloma murine cell line that produces and secretes monoclonal antibodies of the IgG class that substantially specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Claim 5
Dependent Claim
62 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell that produces and secretes monoclonal antibodies of the IgC class that specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of the β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone as intact glycoprotein molecules.
Claim 6
Dependent Claim
62 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell that produces and secretes monoclonal antibodies of the IgC class that specifically bind to the unbound β subunit of human chorionic gonadotropin in the presence of the β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone as intact glycoprotein molecules.
Claim 7
Dependent Claim
66 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin having substantially no inhibition in said binding in the presence of unbound β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
Claim 8
Dependent Claim
66 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin having substantially no inhibition in said binding in the presence of unbound β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
Claim 9
Dependent Claim
103 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin having substantially no cross reactivity with intact human luteinizing hormone, human thyroid stimulating hormone and human follicle stimulating hormone and less than 0.25% cross reactivity with intact human chorionic gonadotropin and having substantially no inhibition in said binding in the presence of unbound β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human follicle stimulating hormone at up to 250 ng/tube.
Claim 10
Dependent Claim
103 words
A murine hybridoma produced by the fusion of a murine lymphocyte and a murine myeloma cell line that produces and secretes monoclonal antibodies of the IgG class that specifically bind to the unbound β subunit of human chorionic gonadotropin having substantially no cross reactivity with intact human luteinizing hormone, human thyroid stimulating hormone and human follicle stimulating hormone and less than 0.25% cross reactivity with intact human chorionic gonadotropin and having substantially no inhibition in said binding in the presence of unbound β subunits of human follicle stimulating hormone, human thyroid stimulating hormone and human follicle stimulating hormone at up to 250 ng/tube.
Claim 11
Independent Claim
51 words
The method of detecting a predominantly β-human chorionic gonadotropin (β-hCG) subunit secreting tumor in vitro, comprising: reacting unbound β-human chorionic gonadotropin (β-hCG) in sera with murine monoclonal antibody of the IgG class specific to said β-hCG in the presence of intact human chorionic gonadotropin and determining the amount of β-hCg-monoclonal antibody.
Claim 12
Independent Claim
51 words
The method of detecting a predominantly β-human chorionic gonadotropin (β-hCG) subunit secreting tumor in vitro, comprising: reacting unbound β-human chorionic gonadotropin (β-hCG) in sera with murine monoclonal antibody of the IgG class specific to said β-hCG in the presence of intact human chorionic gonadotropin and determining the amount of β-hCg-monoclonal antibody.
Claim 13
Independent Claim
47 words
The method of detecting unbound β-human chorionic gonadotropin (β-hCG) by binding said unbound β-hCG to murine monoclonal antibody of the IgG class specific to said β-hCG in the presence of intact human chorionic gonadotropin by means of affinity chromatography and then determining the amount of β-hCG-monoclonal antibody.
Claim 14
Independent Claim
47 words
The method of detecting unbound β-human chorionic gonadotropin (β-hCG) by binding said unbound β-hCG to murine monoclonal antibody of the IgG class specific to said β-hCG in the presence of intact human chorionic gonadotropin by means of affinity chromatography and then determining the amount of β-hCG-monoclonal antibody.
Claim 15
Independent Claim
44 words
The method of radioimmunodetecting a tumor secreting unbound β-human chorionic gonadotropin subunit (β-hCG) comprising contacting said tumor with radiolabeled murine monoclonal antibody of the IgG class specific to said unbound (β-hCG) in the presence of intact human chorionic gonadotropin and detecting the radioactivity emitted.
Claim 16
Independent Claim
44 words
The method of radioimmunodetecting a tumor secreting unbound β-human chorionic gonadotropin subunit (β-hCG) comprising contacting said tumor with radiolabeled murine monoclonal antibody of the IgG class specific to said unbound (β-hCG) in the presence of intact human chorionic gonadotropin and detecting the radioactivity emitted.
Claim 17
Independent Claim
45 words
The method of therapeutically treating a tumor rich in unbound β-human chorionic gonadotropin subunits (β-hCG) comprising contacting said tumor with a therapeutic amount of radiolabeled murine monoclonal antibody of the IgG class specific to said unbound β-hCG in the presence of intact human chorionic gonadotropin.
Claim 18
Independent Claim
45 words
The method of therapeutically treating a tumor rich in unbound β-human chorionic gonadotropin subunits (β-hCG) comprising contacting said tumor with a therapeutic amount of radiolabeled murine monoclonal antibody of the IgG class specific to said unbound β-hCG in the presence of intact human chorionic gonadotropin.
Claim 19
Dependent Claim
67 words
The hybridoma cell line having the essential characteristics of hybridoma cell line KEB-1E5.1, deposited as ATCC HB8095, said characteristics consisting essentially of the production of murine monoclonal antibodies which specifically bind to the unbound β-human chorionic gonadotropin subunit in the presence of intact human chorionic gonadotropin and in the presence of the β subunit of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
Claim 20
Dependent Claim
67 words
The hybridoma cell line having the essential characteristics of hybridoma cell line KEB-1E5.1, deposited as ATCC HB8095, said characteristics consisting essentially of the production of murine monoclonal antibodies which specifically bind to the unbound β-human chorionic gonadotropin subunit in the presence of intact human chorionic gonadotropin and in the presence of the β subunit of human follicle stimulating hormone, human thyroid stimulating hormone and human luteinizing hormone.
Claim 21
Dependent Claim
27 words
A murine antibody of the IgG class that specifically binds to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
Claim 22
Dependent Claim
27 words
A murine antibody of the IgG class that specifically binds to the unbound β subunit of human chorionic gonadotropin in the presence of intact human chorionic gonadotropin.
References Cited (Backward Citations)
US4331647A
Cited
US4361544A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-20 | 1989-07-22 | 1990-01-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-20 | 1993-07-22 | 1994-01-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-20 | 1997-07-22 | 1998-01-21 | $7700.00 | Window Closed / Lapsed |