US4562160A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Melanoma tumor antigen and autologous antibody
Assignee: SLOAN-KETTERING INSTITUTE A CORP OF NY
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Filing: 1983-04-01
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Granted: 1985-12-31
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Expires: 2003-04-01
Abstract
Abstract The present invention concerns novel immunoprecipitating autologous antibodies which recognize the Class 1 gp90 antigen on melanoma cells. These antibodies, optionally tagged with a chromophoric or radioactive label and immobilized on an inert support, may be used to recognize and isolate the gp90 antigen from melanoma cell extracts. Monoclonal antibodies to melanoma may be screened with the gp90 antigen for those which recognize epitopes other than the FD antigenic system. The cell line containing the gp90 antigen which has been cultured in vitro is a source of gp90 antigen for generation of monoclonal antibodies which will be useful in analyzing the gp90 antigen for those epitopes which may be of diagnostic value in immunoassay of melanoma.
Inventors
- 👤 Francisco X. Real
- 👤 M. Jules Mattes
- 👤 Alan N. Houghton
- 👤 Philip O. Livingston
- 👤 Kenneth O. Lloyd
- 👤 Herbert F. Oettgen
- 👤 Lloyd J. Old
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:20
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K16/3053; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S435/961; Y10S436/00; Y10S436/804; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/823; Y10S530/00; Y10S530/806; Y10S530/807; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
17 words
Antibody purified from human serum which specifically binds to Class 1 FD gp 90 melanoma cell antigen.
Claim 2
Dependent Claim
17 words
Antibody purified from human serum which specifically binds to Class 1 FD gp 90 melanoma cell antigen.
Claim 3
Dependent Claim
9 words
Antibody of claim 1, immobilized on a solid support.
Claim 4
Dependent Claim
8 words
Antibody of claim 1 having a radioactive label.
Claim 5
Independent Claim
89 words
A method of detecting the presence or lack of Class 1 FD gp 90 melanoma cell antigen comprising mixing a cell extract thought to contain said antigen with a human serum sample containing antibody which specifically binds to said antigen and incubating under conditions favoring formation of a complex of Class 1 FD gp 90 melanoma cells antigen and antibody specifically binding to said antigen, and determining whether or not a complex of said Class 1 FD gp 90 antigen and antibody specifically binding to said antigen has formed.
Claim 6
Independent Claim
89 words
A method of detecting the presence or lack of Class 1 FD gp 90 melanoma cell antigen comprising mixing a cell extract thought to contain said antigen with a human serum sample containing antibody which specifically binds to said antigen and incubating under conditions favoring formation of a complex of Class 1 FD gp 90 melanoma cells antigen and antibody specifically binding to said antigen, and determining whether or not a complex of said Class 1 FD gp 90 antigen and antibody specifically binding to said antigen has formed.
Claim 7
Dependent Claim
30 words
Method as in claim 4, wherein a complex of Class 1 FD gp 90 antigen and antibody specifically binding to said antigen is formed and is precipitated from said mixture.
Claim 8
Independent Claim
80 words
A method of detecting the presence or lack thereof of monoclonal antibodies which specifically bind to epitopes of Class 1 FD gp 90 melanoma cell antigen comprising contacting a sample thought to contain said antibodies with a sample containing said antigen to form a mixture containing antibodies and antigen and incubating under conditions favoring formation of a complex between said antibodies and said antigen and determining whether or not a complex of said antibodies and said antigen has been formed.
Claim 9
Independent Claim
80 words
A method of detecting the presence or lack thereof of monoclonal antibodies which specifically bind to epitopes of Class 1 FD gp 90 melanoma cell antigen comprising contacting a sample thought to contain said antibodies with a sample containing said antigen to form a mixture containing antibodies and antigen and incubating under conditions favoring formation of a complex between said antibodies and said antigen and determining whether or not a complex of said antibodies and said antigen has been formed.
Claim 10
Independent Claim
68 words
A method of isolating Class 1 FD gp 90 melanoma cell antigen comprising mixing an extract of melanoma cells with a sample of human serum which contains antibody specifically binding to an epitope of said antigen and incubating the mixture under conditions favoring formation of a complex of said antigen and said antibody, precipitating the complex and isolating and separating the antigen and antibody of the precipitated complex.
Claim 11
Independent Claim
68 words
A method of isolating Class 1 FD gp 90 melanoma cell antigen comprising mixing an extract of melanoma cells with a sample of human serum which contains antibody specifically binding to an epitope of said antigen and incubating the mixture under conditions favoring formation of a complex of said antigen and said antibody, precipitating the complex and isolating and separating the antigen and antibody of the precipitated complex.
Claim 12
Dependent Claim
16 words
A method as in claim 7, wherein the melanoma cells are taken from cell line SK-MEL-131.
Claim 13
Dependent Claim
13 words
Class 1 FD gp 90 melanoma cell antigen purified from human melanoma cells.
Claim 14
Dependent Claim
13 words
Class 1 FD gp 90 melanoma cell antigen purified from human melanoma cells.
References Cited (Backward Citations)
US4172124A
Cited
US4361544A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-30 | 1989-07-01 | 1989-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-30 | 1993-07-01 | 1993-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-30 | 1997-07-01 | 1997-12-31 | $7700.00 | Window Closed / Lapsed |