US4551427A
Expired / Lapsed
US
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Process and reagent for the determination of blood glucose in haemolysed whole blood
Assignee: Roche Diagnostics GmbH
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Filing: 1984-01-30
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Granted: 1985-11-05
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Expires: 2004-01-30
Abstract
Abstract A reagent and improved process for the determination of D-glucose in body fluids by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg++ ions and buffer and measurement of the NADPH formed. The improved process requires, before reaction with ATP, the mixing of the whole blood with a reagent which is composed of 0.01 to 0.5% weight/volume of an alkyl sulphate or sulphonate or of an alkyl-aryl sulphonate wherein the alkyl moiety is C8 to C32 and optionally contains one or more hydroxylalkylamino radicals as substituents and/or is interrupted by one or more ether oxygen atoms and the alkyl-aryl radicals having C8 to C18 alkyls and optionally a preserving agent.
Inventors
- 👤 Brigitte Draeger
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 13:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
113 words
In a process for the determination of D-glucose in a blood sample by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ions and buffer and measurement of the NADPH formed, the improvement comprising, before reaction with ATP, the step of mixing the blood sample with 0.01 to 0.5% weight/volume of an alkyl sulphate, an alkyl sulphonate or an alkyl-aryl sulphonate, wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted by one or more hydroxyalkylamino radicals as substituents and are uninterrupted or interrupted by one or more ether-oxygen atoms and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety.
Claim 2
Dependent Claim
113 words
In a process for the determination of D-glucose in a blood sample by reaction with ATP in the presence of hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ions and buffer and measurement of the NADPH formed, the improvement comprising, before reaction with ATP, the step of mixing the blood sample with 0.01 to 0.5% weight/volume of an alkyl sulphate, an alkyl sulphonate or an alkyl-aryl sulphonate, wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted by one or more hydroxyalkylamino radicals as substituents and are uninterrupted or interrupted by one or more ether-oxygen atoms and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety.
Claim 3
Dependent Claim
10 words
Process according to claim 1, wherein dodecyl sulphate is used.
Claim 4
Dependent Claim
16 words
Process of claim 1 wherein a preserving agent is used along with the sulphate or sulphonate.
Claim 5
Dependent Claim
20 words
Process according to claim 3 wherein an alkali metal azide, thiocid, chlorhexidine or imidazoline-urea is used as the preserving agent.
Claim 6
Dependent Claim
10 words
Process of claim 1 wherein an alkyl sulphate is used.
Claim 7
Dependent Claim
10 words
Process of claim 1 wherein an alkyl sulphonate is used.
Claim 8
Dependent Claim
10 words
Process of claim 1 wherein an alkyl-aryl sulphonate is used.
Claim 9
Dependent Claim
10 words
Process of claim 1 wherein an alkyl-phenyl sulphonate is used.
Claim 10
Dependent Claim
138 words
A reagent for use in an improved method for determination of D-glucose in a blood sample of the type wherein the blood sample is reacted with ATP in the presence hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ions and buffer, whereafter the NADPH formed is measured, the improvement being in reacting the blood sample with said reagent before reaction with ATP; said reagent comprising, effective amounts of alkyl sulphate or sulphonate or alkyl-aryl sulphonate, wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted by one or more hydroxyalkylamino radicals as substituents, and are uninterrupted or interrupted by one or more ether-oxygen atoms and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety, and a preserving reagent selected from the group consisting of alkali metal azides, thiocid, chlorhexidine and imidazoline-urea.
Claim 11
Dependent Claim
138 words
A reagent for use in an improved method for determination of D-glucose in a blood sample of the type wherein the blood sample is reacted with ATP in the presence hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ions and buffer, whereafter the NADPH formed is measured, the improvement being in reacting the blood sample with said reagent before reaction with ATP; said reagent comprising, effective amounts of alkyl sulphate or sulphonate or alkyl-aryl sulphonate, wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted by one or more hydroxyalkylamino radicals as substituents, and are uninterrupted or interrupted by one or more ether-oxygen atoms and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety, and a preserving reagent selected from the group consisting of alkali metal azides, thiocid, chlorhexidine and imidazoline-urea.
Claim 12
Dependent Claim
12 words
Reagent of claim 9 consisting of dodecyl sulphate and said preserving agent.
Claim 13
Dependent Claim
21 words
Reagent according to claim 10, wherein it contains dodecyl sulphate and sodium azide in a weight ratio of 0.3 to 5:1.
Claim 14
Dependent Claim
10 words
Reagent of claim 9 wherein an alkyl sulphate is used.
Claim 15
Dependent Claim
10 words
Reagent of claim 9 wherein an alkyl sulphonate is used.
Claim 16
Dependent Claim
10 words
Reagent of claim 9 wherein an alkyl-aryl sulphonate is used.
Claim 17
Dependent Claim
10 words
Reagent of claim 9 wherein an alkyl-aryl sulphonate is used.
Claim 18
Dependent Claim
10 words
Reagent of claim 9 wherein an alkyl-phenyl sulphonate is used.
Claim 19
Dependent Claim
103 words
Test kit for determination of D-glucose in body fluids comprising a first reagent composed of alkyl sulphate, alkyl sulphonate or alkyl-aryl sulphonate wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted one or more times by hydroalkylamino radicals; and are uninterrupted or interrupted one or more times by ether-oxygen atoms; and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety; and a second reagent comprising ATP, hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ion source and buffer, all in effective amounts for determination of D-glucose in body fluids by formation and measurement of NADPH.
Claim 20
Dependent Claim
103 words
Test kit for determination of D-glucose in body fluids comprising a first reagent composed of alkyl sulphate, alkyl sulphonate or alkyl-aryl sulphonate wherein the alkyl radicals contain 8 to 32 carbon atoms and are unsubstituted or substituted one or more times by hydroalkylamino radicals; and are uninterrupted or interrupted one or more times by ether-oxygen atoms; and the alkyl-aryl radicals contain 8 to 18 carbon atoms in the alkyl moiety; and a second reagent comprising ATP, hexokinase, glucose-6-phosphate dehydrogenase, NADP, Mg ++ ion source and buffer, all in effective amounts for determination of D-glucose in body fluids by formation and measurement of NADPH.
References Cited (Backward Citations)
US4416982A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-11-04 | 1989-05-06 | 1989-11-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-11-04 | 1993-05-06 | 1993-11-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-11-04 | 1997-05-06 | 1997-11-05 | $7700.00 | Window Closed / Lapsed |