US4551275A
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Desmethylimipramine derivatives and poly(amino acid) conjugates
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1984-03-23
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Granted: 1985-11-05
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Expires: 2004-03-23
Abstract
Abstract Desmethylimipramine functionalized compounds are provided for conjugation to antigenic compounds, particularly poly(amino acids) and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of desmethylimipramine, while the enzyme conjugate finds use in an enzyme assay for the determination of desmethylimipramine.
Inventors
- 👤 Marcel R. Pirio
- 👤 Prithipal Singh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 17:05
CPC Classifications
E; E21; E21B; E21B19/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9466; C; C07; C07D; C07D223/00; C07D223/14; C07D223/18; C07D223/22; C07D223/24; C07D223/28; E21B17/00; E21B17/02; E21B17/04; E21B17/05; G01N33/53; G01N33/531; Y; Y10; Y10S; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
196 words
A compound of the structure ##STR10## wherein: ψ and ψ' are hydrogen atoms or are taken together to form a double bond to an oxygen atom (oxo) when J is other than a hydrogen atom, being a hydrogen atom when Z is poly(amino acid); Y is a chalcogen (oxygen or sulfur atom) or an imino group; J is a hydrogen atom, or non-oxo-carbonyl, being non-oxo-carbonyl when ψ and ψ' are taken together to form oxo; R is a bond or an aliphatic linking group of from 1 to 18 atoms other than hydrogen atoms comprising carbon, nitrogen, and chalcogen (oxygen and sulfur atoms); Z is an amino group; thiol; thio substituted with alkyl of from 1 to 6 carbon atoms; or poly(amino acid); being a hydrogen atom when r is 0 and ψ and ψ' are taken together to form oxo; m is 0 or 1; r is 0 or 1, being 0 when ψ and ψ' are taken together to form oxo, and being otherwise 1; n is 1 when Z is other than poly(amino aid) and is otherwise a number on the average between 1 and the molecular weight of Z divided by 500.
Claim 2
Dependent Claim
196 words
A compound of the structure ##STR10## wherein: ψ and ψ' are hydrogen atoms or are taken together to form a double bond to an oxygen atom (oxo) when J is other than a hydrogen atom, being a hydrogen atom when Z is poly(amino acid); Y is a chalcogen (oxygen or sulfur atom) or an imino group; J is a hydrogen atom, or non-oxo-carbonyl, being non-oxo-carbonyl when ψ and ψ' are taken together to form oxo; R is a bond or an aliphatic linking group of from 1 to 18 atoms other than hydrogen atoms comprising carbon, nitrogen, and chalcogen (oxygen and sulfur atoms); Z is an amino group; thiol; thio substituted with alkyl of from 1 to 6 carbon atoms; or poly(amino acid); being a hydrogen atom when r is 0 and ψ and ψ' are taken together to form oxo; m is 0 or 1; r is 0 or 1, being 0 when ψ and ψ' are taken together to form oxo, and being otherwise 1; n is 1 when Z is other than poly(amino aid) and is otherwise a number on the average between 1 and the molecular weight of Z divided by 500.
Claim 3
Dependent Claim
27 words
The compound of claim 1 wherein ψ and ψ' are taken together to form oxo, r is 0, Z is a hydrogen atom, and J is non-oxo-carbonyl.
Claim 4
Dependent Claim
22 words
The compound of claim 1 wherein ψ and ψ' are hydrogen atoms, r is 0, Z is amino, and J is non-oxo-carbonyl.
Claim 5
Dependent Claim
106 words
A compound of the formula: ##STR11## wherein: R 2 is ##STR12## M is amino; Q is C═W wherein W is an oxygen atom, an imino group, or a sulfur atom; T and T' are linking groups of from 1 to 4 carbon atoms; Y and Y' are, respectively an oxygen atom, an imino group, or a sulfur atom; A is an amino group or an oxygen atom; a, k, p, and m 2 are, respectively, 0 or 1; n 2 is at least 1 and on the average not greater than the molecular weight of Z 2 divided by 500; and Z 2 is poly(amino acid).
Claim 6
Dependent Claim
106 words
A compound of the formula: ##STR11## wherein: R 2 is ##STR12## M is amino; Q is C═W wherein W is an oxygen atom, an imino group, or a sulfur atom; T and T' are linking groups of from 1 to 4 carbon atoms; Y and Y' are, respectively an oxygen atom, an imino group, or a sulfur atom; A is an amino group or an oxygen atom; a, k, p, and m 2 are, respectively, 0 or 1; n 2 is at least 1 and on the average not greater than the molecular weight of Z 2 divided by 500; and Z 2 is poly(amino acid).
Claim 7
Dependent Claim
10 words
The compound of claim 4 wherein R 2 is ##STR13##
Claim 8
Dependent Claim
11 words
The compound of claim 4 wherein Z 2 is an antigen.
Claim 9
Dependent Claim
13 words
The compound of claim 4 wherein Z 2 is an bovine serum albumin.
Claim 10
Dependent Claim
12 words
The compound of claim 4 wherein Z 2 is bovine gamma globulin.
Claim 11
Dependent Claim
11 words
The compound of claim 4 wherein Z 2 is an enzyme.
Claim 12
Dependent Claim
11 words
The compound of claim 4 wherein Z 2 is glucose-6-phosphate dehydrogenase.
Claim 13
Dependent Claim
44 words
Antibodies produced in response to the compound of claim 4 wherein Z 2 is an antigen, which antibodies bind to the compound of claim 4 wherein Z 2 is an antigen, the compound of claim 4 wherein Z 2 is an enzyme and desmethylimipramine.
Claim 14
Dependent Claim
33 words
A compound of the formula: ##STR14## wherein: J' is a hydrogen atom or non-oxo-carbonyl, R 1 1 is amino ##STR15## Z is a hydrogen atom or thiol of 0 to 6 carbon atoms.
Claim 15
Dependent Claim
33 words
A compound of the formula: ##STR14## wherein: J' is a hydrogen atom or non-oxo-carbonyl, R 1 1 is amino ##STR15## Z is a hydrogen atom or thiol of 0 to 6 carbon atoms.
Claim 16
Dependent Claim
42 words
A compound of the formula: ##STR16## wherein: P is 0 or 1; n 2 is at least 1 and on the average not greater than the molecular weight of PAA divided by 500; and PAA is a poly(amino acid) antigen or enzyme.
Claim 17
Dependent Claim
42 words
A compound of the formula: ##STR16## wherein: P is 0 or 1; n 2 is at least 1 and on the average not greater than the molecular weight of PAA divided by 500; and PAA is a poly(amino acid) antigen or enzyme.
Claim 18
Dependent Claim
21 words
Antibodies produced in response to the compound of claim 13 which bind to the compound of claim 13 and to desmethylimipramine.
Claim 19
Dependent Claim
11 words
The compound of claim 13 wherein PAA is bovine serum albumin.
Claim 20
Dependent Claim
11 words
The compound of claim 13 wherein PAA is bovine gamma globulin.
Claim 21
Dependent Claim
10 words
The compound of claim 13 wherein PAA is glucose-6-phosphate dehydrogenase.
Claim 22
Dependent Claim
94 words
A method for determining the presence of desmethylimipramine in a sample suspected of containing desmethylimipramine, which comprises: (a) combining the sample in an aqueous medium with (1) an antibody produced in response to the compound of claim 4, wherein Z 2 is an antigen and (2) the compound of claim 4 wherein Z 2 is an enzyme, (b) determining the enzyme activity of the combination, and (c) determining the presence of desmethylimipramine in the sample by comparing the enzyme activity with the enzyme activity of an assay medium having a known amount of desmethylimipramine.
Claim 23
Dependent Claim
44 words
In an enzyme immunoassay method for determining desmethylimipramine, the improvement which comprises employing (1) an antibody produced in response to the compound of claim 4 wherein Z 2 is an antigen and (2) the compound of claim 4 wherein Z 2 is an enzyme.
References Cited (Backward Citations)
US4275160A
Cited
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