US4545987A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Psoralen inactivated double-stranded RNA viral vaccines
Assignee: Bayer Corp
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Filing: 1983-12-20
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Granted: 1985-10-08
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Expires: 2003-12-20
Abstract
Abstract Novel vaccines of double-stranded RNA viruses are prepared by psoralen inactivation under mild conditions in an inert atmosphere, optionally in the presence of a mild singlet oxygen scavenger. The resulting inactivated virus can be used as a vaccine for inoculation of hosts to provide for the stimulation of the immune system to the virus.
Inventors
- 👤 Richard E. Giles
- 👤 David R. Stevens
- 👤 Gary P. Wiesehahn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:39
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61K; A61K39/00; A61K39/12; A61K39/15; A61K2039/51; A61K2039/525; A61K2039/5252; A61K2039/545; A61K2039/555; A61K2039/55505; A61K2039/55511; A61K2039/55566; C12N2720/00; C12N2720/00011; C12N2720/12011; C12N2720/12111; C12N2720/12134; C12N2720/12161; C12N2720/12163; Y; Y10; Y10S; Y10S424/00; Y10S424/813; Y10S424/814
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
79 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10 6 to 10 9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree, and an immunologic adjuvant.
Claim 2
Dependent Claim
79 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10 6 to 10 9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree, and an immunologic adjuvant.
Claim 3
Dependent Claim
11 words
A vaccine according to claim 1, wherein said furocoumarin is 4'-aminomethyl-4,5',8-trimethylpsoralen.
Claim 4
Dependent Claim
13 words
A vaccine according to claim 2, wherein said BTV is of serotype 11.
Claim 5
Dependent Claim
19 words
A vaccine according to claim 1, wherein said BTV is inactivated in the presence of a singlet oxygen scavenger.
Claim 6
Dependent Claim
17 words
A vaccine according to claim 1, wherein said inactivation is performed in the substantial absence of oxygen.
Claim 7
Dependent Claim
21 words
A vaccine according to claim 1, wherein said BTV is grown in substantially confluent monolayers of cells immediately prior to inactivation.
Claim 8
Dependent Claim
73 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by irradiation with long wavelength ultraviolet light at a temperature in the range of about -10° to 25° C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of about 10 6 to 10 9 pfu/ml, and an immunologic adjuvant.
Claim 9
Dependent Claim
73 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by irradiation with long wavelength ultraviolet light at a temperature in the range of about -10° to 25° C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of about 10 6 to 10 9 pfu/ml, and an immunologic adjuvant.
Claim 10
Dependent Claim
75 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10 6 to 10 9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree.
Claim 11
Dependent Claim
75 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises at least one furocoumarin-inactivated BTV serotype in from about 10 6 to 10 9 pfu/ml, wherein said inactivation is as a result of irradiation of BTV in the presence of an inactivating furocoumarin with long wavelength ultraviolet light at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree.
Claim 12
Dependent Claim
60 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by temperature in the range of about -10° to 25° C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of 10 6 to 10 9 pfu/ml.
Claim 13
Dependent Claim
60 words
A vaccine useful for inoculation of a mammalian host susceptible to infection by Bluetongue virus (BTV), which comprises BTV serotype 11 inactivated with 4'-aminomethyl-4,5',8-trimethylpsoralen by temperature in the range of about -10° to 25° C. for a time sufficient to inactivate said BTV to become non-infectious, said BTV being present in an amount of 10 6 to 10 9 pfu/ml.
Claim 14
Independent Claim
69 words
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises inactivating at least one BTV serotype by exposure to long wavelength ultraviolet light in the presence of a furocoumarin at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree, and combining said inactivated BTV with an appropriate adjuvent.
Claim 15
Independent Claim
69 words
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises inactivating at least one BTV serotype by exposure to long wavelength ultraviolet light in the presence of a furocoumarin at a temperature below about 40° C. for a time sufficient to inactivate said BTV to a non-infectious degree, and combining said inactivated BTV with an appropriate adjuvent.
Claim 16
Independent Claim
74 words
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises exposure of at least one BTV serotype to long wavelength ultraviolet light in the presence of 4'-aminomethyl-4,5', 8-trimethylpsoralen at a temperature in the range from about -10° C. to 25° C. for a time sufficient to inactivate the BTV to a non-infectious degree, and combining said inactivated BTV with a suitable adjuvant.
Claim 17
Independent Claim
74 words
A method for producing a vaccine for inoculation of a mammalian host susceptible to infection by bluetongue virus (BTV), which method comprises exposure of at least one BTV serotype to long wavelength ultraviolet light in the presence of 4'-aminomethyl-4,5', 8-trimethylpsoralen at a temperature in the range from about -10° C. to 25° C. for a time sufficient to inactivate the BTV to a non-infectious degree, and combining said inactivated BTV with a suitable adjuvant.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-10-07 | 1989-04-08 | 1989-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-10-07 | 1993-04-08 | 1993-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-10-07 | 1997-04-08 | 1997-10-08 | $7700.00 | Window Closed / Lapsed |