US4534996A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hybrid microspheres
Assignee: California Institute of Technology
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Filing: 1983-08-29
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Granted: 1985-08-13
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Expires: 2003-08-29
Abstract
Abstract Substrates, particularly inert synthetic organic resin beads (10) or sheet (12) such as polystyrene are coated with a covalently bound layer (24) of polyacrolein by irradiation a solution (14) of acrolein or other aldehyde with high intensity radiation. Individual microspheres (22) are formed which attach to the surface to form the aldehyde containing layer (24). The aldehyde groups can be converted to other functional groups by reaction with materials such as hydroxylamine. Adducts of proteins such as antibodies or enzymes can be formed by direct reaction with the surface aldehyde groups.
Inventors
- 👤 Alan Rembaum
- 👤 Richard C. K. Yen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 02:19
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54313; G01N33/54326; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
88 words
A method of forming a coated product comprising the steps of: immersing a synthetic organic resin substrate in the form of spheres, particles, sheets, rods or tubes in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms; irradiating the solution with high energy radiation to form an addition polymerized polyaldehyde layer which is covalently bound to the organic resin surface of the substrate; and binding an antibody to the polyaldehyde layer to form a cell specific surface.
Claim 2
Independent Claim
88 words
A method of forming a coated product comprising the steps of: immersing a synthetic organic resin substrate in the form of spheres, particles, sheets, rods or tubes in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms; irradiating the solution with high energy radiation to form an addition polymerized polyaldehyde layer which is covalently bound to the organic resin surface of the substrate; and binding an antibody to the polyaldehyde layer to form a cell specific surface.
Claim 3
Dependent Claim
36 words
A method according to claim 1 in which the solution further contains from 1 to 50% by weight of a water soluble solvent for the surface of the substrate which is a non-solvent for the coating.
Claim 4
Dependent Claim
18 words
A method according to claim 2 in which the solvent is selected from dimethyl sulfoxide, tetrahydrofuran and dioxane.
Claim 5
Dependent Claim
20 words
A method according to claim 1 in which the resin is selected from polyethylene, polypropylene, polystyrene, acrylic polymers or dextran.
Claim 6
Dependent Claim
26 words
A method according to claim 4 in which the aldehyde is selected from acrolein and C 1 to C 8 aryl, alkyl and cycloalkyl derivatives thereof.
Claim 7
Dependent Claim
12 words
A method according to claim 5 in which the aldehyde is acrolein.
Claim 8
Dependent Claim
26 words
A method according to claim 6 further including at least 20% of an additional polymerizable comonomer having a hydrophilic substituent selected from hydroxyl, amino or carboxyl.
Claim 9
Dependent Claim
14 words
A method according to claim 1 in which the solution contains a suspending agent.
Claim 10
Dependent Claim
30 words
A method according to claim 8 in which the suspending agent is selected from polyalkylene oxide liquid polymers and an alkali metal alkyl sulfate containing 8 to 20 carbon atoms.
Claim 11
Dependent Claim
20 words
A method according to claim 9 in which the agent is selected from sodium lauryl sulfate and sodium dodecyl sulfate.
Claim 12
Dependent Claim
32 words
A method according to claim 1 further including the steps of: contacting said surface with a suspension of animal cells; and binding those cells containing conjugate antigen receptor sites to said surface.
Claim 13
Independent Claim
120 words
A method of forming a coated article comprising the steps of: immersing an article having a surface comprising a synthetic organic resin in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms and containing a suspending agent, said organic resin being capable of developing covalent bonds during high energy irradiation; irradiating the solution with high energy radiation to polymerize said aldehyde in the form of individual microspheres having a uniform diameter; and attaching said microspheres to the surface of said article in the form of a continuous layer of contiguous, tangential microspheres by covalent bonds formed between said resin and the microspheres by means of said high energy radiation.
Claim 14
Independent Claim
120 words
A method of forming a coated article comprising the steps of: immersing an article having a surface comprising a synthetic organic resin in an aqueous solution containing up to 20 percent by weight of an unsaturated aldehyde containing 4 to 20 carbon atoms and containing a suspending agent, said organic resin being capable of developing covalent bonds during high energy irradiation; irradiating the solution with high energy radiation to polymerize said aldehyde in the form of individual microspheres having a uniform diameter; and attaching said microspheres to the surface of said article in the form of a continuous layer of contiguous, tangential microspheres by covalent bonds formed between said resin and the microspheres by means of said high energy radiation.
Claim 15
Dependent Claim
25 words
A method according to claim 12 in which the aldehyde is polymerized to form microspheres having a uniform diameter between 100 Angstroms and 2000 Angstroms.
Claim 16
Dependent Claim
21 words
A method according to claim 12 in which the article is in the form of spheres, particles, sheets, rods or tubes.
Claim 17
Dependent Claim
20 words
A method according to claim 14 in which the resin is selected from polyethylene, polypropylene, polystyrene, acrylic polymers or dextran.
Claim 18
Dependent Claim
26 words
A method according to claim 15 in which the aldehyde is selected from acrolein and C 1 to C 8 aryl, alkyl and cycloalkyl derivatives thereof.
Claim 19
Dependent Claim
12 words
A method according to claim 16 in which the aldehyde is acrolein.
Claim 20
Dependent Claim
30 words
A method according to claim 12 in which the suspending agent is selected from polyalkylene oxide liquid polymers and an alkali metal alkyl sulfate containing 8 to 20 carbon atoms.
Claim 21
Dependent Claim
20 words
A method according to claim 18 in which the agent is selected from sodium lauryl sulfate and sodium dodecyl sulfate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-12 | 1989-02-11 | 1989-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-12 | 1993-02-11 | 1993-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-12 | 1997-02-11 | 1997-08-13 | $7700.00 | Window Closed / Lapsed |