US4529694A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell fusion
Assignee: Boston Children's Hospital
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Filing: 1982-04-16
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Granted: 1985-07-16
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Expires: 2002-04-16
Abstract
Abstract Human hybridomas producing a preselected human monoclonal antibody are prepared by fusing human lymphocytes with a hybrid fusion partner. The hybrid fusion partner is the result of fusing human lymphocytes with human myeloma cells at least once wherein the resulting hybrid cell is capable of being a functional human fusion partner.
Inventors
- 👤 Herbert Lazarus
- 👤 Jerrold F. Schwaber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:49
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/10; C12N5/12; C12N5/16; C12N5/163; C07; C07K; C07K16/00; C07K16/12; C07K16/1267; C07K16/1275; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
13 words
A human myeloma analog cell from the strain deposited as ATCC HB 8121.
Claim 2
Dependent Claim
13 words
A human myeloma analog cell from the strain deposited as ATCC HB 8121.
Claim 3
Independent Claim
67 words
A method of producing an antibody to a particular antigenic determinant, said method comprising the steps of: (a) obtaining a mammalian lymphocyte capable of producing said antibody, (b) fusing said lymphocyte to a human myeloma analog cell from the strain deposited as ATCC HB 8121 to form a hybrid cell, (c) culturing said hybrid cell in culture media, and (d) recovering said antibody from said culture media.
Claim 4
Independent Claim
67 words
A method of producing an antibody to a particular antigenic determinant, said method comprising the steps of: (a) obtaining a mammalian lymphocyte capable of producing said antibody, (b) fusing said lymphocyte to a human myeloma analog cell from the strain deposited as ATCC HB 8121 to form a hybrid cell, (c) culturing said hybrid cell in culture media, and (d) recovering said antibody from said culture media.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein said lymphocyte is of human origin.
Claim 6
Dependent Claim
15 words
The method of claim 2 or claim 3 wherein said lymphocyte is a spleen cell.
Claim 7
Dependent Claim
27 words
The method of claim 2 wherein said lymphocyte capable of producing said antibody is taken from a mammal which has been immunized with said particular antigenic determinant.
Claim 8
Independent Claim
182 words
A method of producing a competent human myeloma analog cell capable of supporting the production of antibody when fused to mammalian lymphocyte while failing to produce antibody of its own, said method comprising the steps of: (a) providing an incompetent human myeloma cell incapable of supporting the production of antibody when fused to a mammalian lymphocyte; (b) fusing said incompetent cell to a human lymphocyte to form a hybrid cell; (c) determining whether said hybrid cell is competent and fails to produce its own antibody and is thus said competent human myeloma analog cell; (d) if said hybrid fails to produce its own antibody but is incompetent, fusing said incompetent hybrid cell to an additional human lymphocyte to form a further hybrid cell, and determining whether said further hybrid cell is competent and fails to produce its own antibody and is thus said competent human myeloma analog cell; and (e) if said further hybrid cell fails to produce its own antibody but is incompetent, repeating said fusing and determining steps of (d) above until said competent human myeloma analog cell is produced.
Claim 9
Independent Claim
182 words
A method of producing a competent human myeloma analog cell capable of supporting the production of antibody when fused to mammalian lymphocyte while failing to produce antibody of its own, said method comprising the steps of: (a) providing an incompetent human myeloma cell incapable of supporting the production of antibody when fused to a mammalian lymphocyte; (b) fusing said incompetent cell to a human lymphocyte to form a hybrid cell; (c) determining whether said hybrid cell is competent and fails to produce its own antibody and is thus said competent human myeloma analog cell; (d) if said hybrid fails to produce its own antibody but is incompetent, fusing said incompetent hybrid cell to an additional human lymphocyte to form a further hybrid cell, and determining whether said further hybrid cell is competent and fails to produce its own antibody and is thus said competent human myeloma analog cell; and (e) if said further hybrid cell fails to produce its own antibody but is incompetent, repeating said fusing and determining steps of (d) above until said competent human myeloma analog cell is produced.
Claim 10
Dependent Claim
17 words
The method of claim 6 wherein said fusing and determining steps of step (d) are repeated once.
Claim 11
Dependent Claim
39 words
A hybrid cell capable of producing an antibody to a particular antigenic determinant, said cell comprising a human myeloma analog cell from the strain deposited as ATCC HB 8121 fused to a mammalian lymphocyte capable of producing said antibody.
Claim 12
Dependent Claim
39 words
A hybrid cell capable of producing an antibody to a particular antigenic determinant, said cell comprising a human myeloma analog cell from the strain deposited as ATCC HB 8121 fused to a mammalian lymphocyte capable of producing said antibody.
Claim 13
Dependent Claim
15 words
The hybrid cell of claim 8 wherein said mammalian lymphocyte is a human spleen cell.
Claim 14
Dependent Claim
20 words
The hybrid cell of claim 9 wherein said human spleen cell is committed to producing antibody specific to pneumococcal polysaccharide.
References Cited (Backward Citations)
GB2079313A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-15 | 1989-01-14 | 1989-07-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-15 | 1993-01-14 | 1993-07-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-15 | 1997-01-14 | 1997-07-16 | $7700.00 | Window Closed / Lapsed |