US4526690A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus for nucleic acid quantification
Assignee: EMD Millipore Corp
•
Filing: 1983-02-04
•
Granted: 1985-07-02
•
Expires: 2003-02-04
Abstract
Abstract A multiwell apparatus for the assay of microliter quantities of body fluids is provided which prevents fluid loss by lateral migration or gravity flow through a microporous membrane or ultrafilter. A sample is passed through a membrane to which an antibody has been bonded in order to harvest complementary viral antigens in the sample. The harvested viral antigen is converted to single strand DNA or RNA which then is deposited on a second membrane. The deposited single strand DNA or RNA is capable of reacting with labeled DNA or other suitable detection probe which can be detected and correlated with antigen concentration in the sample.
Inventors
- 👤 Joseph R. Kiovsky
- 👤 Clifford L. Hendrick
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 05:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5302; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6804; C12Q1/70; G01N33/5304; G01N33/543; G01N33/544; G01N33/548
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
159 words
Apparatus comprising: a plate having a thickness and at least one aperture extending through the thickness of the plate; a first membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and having an antibody coupled to said first membrane and disposed over one end of said at least one aperture in said plate such that at least one well having a discrete volume is formed; a hydrophobic fabric contiguous to said first membrane at said one end; a second plate defining at least one chamber, one of said at least one chamber being associated with one of said at least one aperture to receive all liquid passing through said at least one aperture, each said at least one chamber having a bottom comprising a second membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and being capable of coupling to a single strand RNA or single strand DNA molecule.
Claim 2
Dependent Claim
159 words
Apparatus comprising: a plate having a thickness and at least one aperture extending through the thickness of the plate; a first membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and having an antibody coupled to said first membrane and disposed over one end of said at least one aperture in said plate such that at least one well having a discrete volume is formed; a hydrophobic fabric contiguous to said first membrane at said one end; a second plate defining at least one chamber, one of said at least one chamber being associated with one of said at least one aperture to receive all liquid passing through said at least one aperture, each said at least one chamber having a bottom comprising a second membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and being capable of coupling to a single strand RNA or single strand DNA molecule.
Claim 3
Dependent Claim
203 words
Apparatus comprising: a plate having a thickness and at least one aperture extending through the thickness of the plate; a first membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and having an antibody coupled to said first membrane and disposed across one end of said at least one aperture in said plate such that at least one well having a discrete volume is formed; a hydrophobic fabric contiguous to said first membrane at said one end; a second plate defining at least one chamber, one of said at least one chamber being associated with one of said at least one aperture, and positioned with respect to said at least one aperture to receive all liquid passing through said at least one aperture, each said at least one chamber having a bottom comprising a second membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and being capable of coupling to a single strand RNA or single strand DNA molecule and; a guiding projection aligned with each said at least one aperture and positioned beneath said first membrane to direct fluid passing through said first membrane to one of said at least one chamber.
Claim 4
Dependent Claim
203 words
Apparatus comprising: a plate having a thickness and at least one aperture extending through the thickness of the plate; a first membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and having an antibody coupled to said first membrane and disposed across one end of said at least one aperture in said plate such that at least one well having a discrete volume is formed; a hydrophobic fabric contiguous to said first membrane at said one end; a second plate defining at least one chamber, one of said at least one chamber being associated with one of said at least one aperture, and positioned with respect to said at least one aperture to receive all liquid passing through said at least one aperture, each said at least one chamber having a bottom comprising a second membrane selected from the group consisting of a microporous membrane and an ultrafiltration membrane and being capable of coupling to a single strand RNA or single strand DNA molecule and; a guiding projection aligned with each said at least one aperture and positioned beneath said first membrane to direct fluid passing through said first membrane to one of said at least one chamber.
Claim 5
Independent Claim
29 words
The apparatus of any one of claims 1 or 2 wherein said first membrane is cellulose acetate and wherein said antibody is coupled to said first membrane with divinylsulfone.
Claim 6
Independent Claim
16 words
The apparatus of any one of claims 1 or 2 wherein said second membrane is nitrocellulose.
Claim 7
Independent Claim
19 words
The apparatus of any one of claims 1 or 2 wherein said antibody is an antibody to a virus.
Claim 8
Dependent Claim
22 words
The apparatus as recited in claim 1 or 2 wherein said first membrane is cellulose acetate and said second membrane is nitrocellulose.
Claim 9
Dependent Claim
26 words
The apparatus as recited in claim 1 or 2 wherein said hydrophobic fabric is selected from the group consisting of a polyester, a polyolefin and polytetrafluoroethylene.
Claim 10
Dependent Claim
16 words
The apparatus as recited in claim 1 or 2 wherein said hydrophobic fabric is heat bondable.
Claim 11
Dependent Claim
17 words
The apparatus as recited in claim 1 or 2 wherein said hydrophobic fabric is secured with adhesive.
Claim 12
Dependent Claim
17 words
The apparatus as recited in claim 1 or 2 wherein antibody is an antibody to a virus.
References Cited (Backward Citations)
US30562A
Cited
US3649464A
Cited
US4154795A
Cited
US4245064A
Cited
US4220535A
Cited
US4304865A
Cited
US4246339A
Cited
USRE30562E
Cited
US4276048A
Cited
US4302204A
Cited
US4338094A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-01 | 1988-12-31 | 1989-07-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-01 | 1992-12-31 | 1993-07-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-01 | 1996-12-31 | 1997-07-02 | $7700.00 | Window Closed / Lapsed |