US4525456A
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Support matrix and immobilized enzyme system
Assignee: Honeywell UOP LLC
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Filing: 1982-11-08
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Granted: 1985-06-25
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Expires: 2002-11-08
Abstract
Abstract A support matrix which immobilizes enzymes by ion exchange forces comprises a core support deposited on which is a functionalized polyethylenimine insoluble in water. Such support matrices may be prepared from a virtually limitless variety of core supports since the functionalized polyethylenimines of this invention show excellent adhesive properties even to smooth surfaces. Such support matrices are particularly useful where the enzyme to be immobilized has a rather limited inherent half-life. The functionalized polyethylenimine is a carboxylic acid amide of polyethylenimine, a sulfonic acid amide of polyethylenimine or polyalkylated polyethylenimine.
Inventors
- 👤 Ronald P. Rohrbach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:06
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/098; C12N11/14
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
55 words
A method of preparing a support matrix comprising depositing a water-insoluble functionalized polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimine on a core support, and recovering the resulting support matrix.
Claim 2
Independent Claim
55 words
A method of preparing a support matrix comprising depositing a water-insoluble functionalized polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimine on a core support, and recovering the resulting support matrix.
Claim 3
Dependent Claim
38 words
The method of claim 1 where the functionalized polyethylenimine is a carboxylic or sulfonic acid amide of polyethylenimine wherein substantially all primary amino groups and a major proportion of the secondary amino groups have been converted to amides.
Claim 4
Dependent Claim
22 words
The method of claim 2 where the carboxylic acid is a saturated aliphatic carboxylic acid containing more than about 4 carbon atoms.
Claim 5
Dependent Claim
14 words
The method of claim 2 where the carboxylic acid is an aromatic carboxylic acid.
Claim 6
Dependent Claim
11 words
The method of claim 4 where the acid is benzoic acid.
Claim 7
Dependent Claim
14 words
The method of claim 2 where the sulfonic acid is an aromatic sulfonic acid.
Claim 8
Dependent Claim
12 words
The method of claim 6 where the acid is benzene sulfonic acid.
Claim 9
Dependent Claim
12 words
The method of claim 6 where the acid is toluene sulfonic acid.
Claim 10
Dependent Claim
24 words
The method of claim 1 where the core support is selected from the group consisting of inorganic oxides, glass, ceramics, metals, plastics and membranes.
Claim 11
Dependent Claim
50 words
A support matrix comprising a core support on whose surface is deposited a water-insoluble functionalized derivative of polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimne.
Claim 12
Dependent Claim
50 words
A support matrix comprising a core support on whose surface is deposited a water-insoluble functionalized derivative of polyethylenimine, where the polyethylenimine has a molecular weight from about 1200 to about 100,000, selected from the group consisting of carboxylic acid amides of polyethylenimine, sulfonic acid amides of polyethylenimine, and polyalkylated polyethylenimne.
Claim 13
Dependent Claim
40 words
The support matrix of claim 10 where the functionalized polyethylenimine is a carboxylic or sulfonic acid amide of polyethylenimine wherein substantially all the primary amino groups and a major proportion of the secondary amino groups have been converted to amides.
Claim 14
Dependent Claim
23 words
The support matrix of claim 11 where the carboxylic acid is a saturated aliphatic carboxylic acid containing more than about 4 carbon atoms.
Claim 15
Dependent Claim
15 words
The support matrix of claim 11 where the carboxylic acid is an aromatic carboxylic acid.
Claim 16
Dependent Claim
12 words
The support matrix of claim 13 where the acid is benzoic acid.
Claim 17
Dependent Claim
15 words
The support matrix of claim 11 where the sulfonic acid is an aromatic sulfonic acid.
Claim 18
Dependent Claim
13 words
The support matrix of claim 15 where the acid is benzene sulfonic acid.
Claim 19
Dependent Claim
13 words
The support matrix of claim 15 where the acid is toluene sulfonic acid.
Claim 20
Dependent Claim
25 words
The support matrix of claim 10 where the core support is selected from the group consisting of inorganic oxides, glass, ceramics, metals, plastics and membranes.
Claim 21
Dependent Claim
18 words
An immobilized enzyme system comprising the support matrix of claim 10 to which there is bound an enzyme.
Claim 22
Dependent Claim
37 words
The immobilized enzyme system of claim 19 where the enzyme is selected from the group consisting of glucoamylase, alphaamylase, beta-amylase, pullulanase, lactase, rennin, glucose oxidase, protease, lipase, urease, arginase, asparaginase, catalase, chymotrypsin, cellulase, peroxidase, lysozyme, and papain.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-24 | 1988-12-24 | 1989-06-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-24 | 1992-12-24 | 1993-06-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-24 | 1996-12-24 | 1997-06-25 | $7700.00 | Window Closed / Lapsed |