US4520111A
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Diagnostic method for determining content of sialic acid or sialoprotein
Assignee: Research Corp
•
Filing: 1984-01-26
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Granted: 1985-05-28
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Expires: 2004-01-26
Abstract
Abstract A hemagglutinate lectin derived from the slug, Limax flavus, having a high degree of hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoproteins and diagnostic and separatory methods and compositions based upon the inhibitory effect of sialoproteins or sialic acid on the hemagglutinating properties of the slug-derived lectin.
Inventors
- 👤 Ronald L. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 06:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S436/827; Y10S530/00; Y10S530/855; Y10S530/858
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
148 words
A diagnostic method for determining the content of sialic acid or sialoprotein in a biological liquid comprising contacting the said biological liquid with a predetermined quantity of a substantially homogeneous lectin of the slug Limax flavus having a high degree of hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoprotein having an approximate amino acid composition: ______________________________________
Moles per 22,000 g of Lectin
Amino Acid (Nearest integer)
______________________________________
Asx 26
Thr 13
Ser 13
Glx 14
Pro 4
Gly 22
Ala 16
Val 7
Met 2
Ile 7
Leu 15
Tyr 10
Phe 10
His 7
Lys 17
Trp 6
Arg 8
Cys 6
______________________________________ and measuring the degree of hemagglutination effected by said lectin and comparing said measurement with the degree of hemagglutination effected with contacting an identical quantity of said lectin with a similar biological fluid containing a known amount of sialic acid or sialoprotein.
Claim 2
Dependent Claim
148 words
A diagnostic method for determining the content of sialic acid or sialoprotein in a biological liquid comprising contacting the said biological liquid with a predetermined quantity of a substantially homogeneous lectin of the slug Limax flavus having a high degree of hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoprotein having an approximate amino acid composition: ______________________________________
Moles per 22,000 g of Lectin
Amino Acid (Nearest integer)
______________________________________
Asx 26
Thr 13
Ser 13
Glx 14
Pro 4
Gly 22
Ala 16
Val 7
Met 2
Ile 7
Leu 15
Tyr 10
Phe 10
His 7
Lys 17
Trp 6
Arg 8
Cys 6
______________________________________ and measuring the degree of hemagglutination effected by said lectin and comparing said measurement with the degree of hemagglutination effected with contacting an identical quantity of said lectin with a similar biological fluid containing a known amount of sialic acid or sialoprotein.
Claim 3
Dependent Claim
23 words
The diagnostic method of claim 1 wherein the degree of hemagglutination is measured by determining the optical density of the treated biological liquid.
Claim 4
Dependent Claim
13 words
The diagnostic method of claim 1 wherein said biological liquid is whole blood.
Claim 5
Dependent Claim
12 words
The diagnostic method of claim 1 wherein said biological liquid is plasma.
Claim 6
Dependent Claim
14 words
The diagnostic method of claim 1 wherein said biological liquid is serum or urine.
Claim 7
Dependent Claim
14 words
The diagnostic method of claim 1 wherein said liquid is tissue or cellular homogenate.
References Cited (Backward Citations)
US4115062A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-27 | 1988-11-26 | 1989-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-27 | 1992-11-26 | 1993-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-27 | 1996-11-26 | 1997-05-28 | $7700.00 | Window Closed / Lapsed |