US4518694A
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Aqueous compositions containing stabilized enzymes
Assignee: Colgate Palmolive Co
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Filing: 1982-09-03
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Granted: 1985-05-21
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Expires: 2002-09-03
Abstract
Abstract Aqueous enzyme preparation stabilized with a salt of a lower molecular weight acid in the presence of alcohol.
Inventors
- 👤 Elias H. Shaer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:06
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/96; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38663
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
196 words
An aqueous-based enzyme containing composition wherein the enzymes have enhanced stability against loss of activity, the composition comprising on a weight basis: (a) from about 0 to about 55% of a surfactant selected from the group consisting of anionic and nonionic surfactants, and mixtures thereof; (b) from about 0.006 to about 5% of an active enzyme selected from the group consisting of protease and alpha amylase enzymes, and mixtures thereof, said enzyme being provided in pure form or as incorporated within a commercial enzyme preparation comprising from about 2 to about 80% of said enzyme and from about 20 to about 98% of a carrier therefor; (c) an enzyme stabilizer consisting essentially of on a weight basis of said composition: (i) from about 0.5 to about 15% of a salt of a lower molecular weight organic acid selected from the group consisting of alkali metal, ammonium and alkanol ammonium salts of formic, acetic, propionic, butyric, and valeric acids, and mixtures thereof, and (ii) from about 1.0 to about 25% of an alcohol having the formula ROH wherein R is an alkyl having one to six carbon atoms, and (d) from about 10 to about 90% water.
Claim 2
Dependent Claim
196 words
An aqueous-based enzyme containing composition wherein the enzymes have enhanced stability against loss of activity, the composition comprising on a weight basis: (a) from about 0 to about 55% of a surfactant selected from the group consisting of anionic and nonionic surfactants, and mixtures thereof; (b) from about 0.006 to about 5% of an active enzyme selected from the group consisting of protease and alpha amylase enzymes, and mixtures thereof, said enzyme being provided in pure form or as incorporated within a commercial enzyme preparation comprising from about 2 to about 80% of said enzyme and from about 20 to about 98% of a carrier therefor; (c) an enzyme stabilizer consisting essentially of on a weight basis of said composition: (i) from about 0.5 to about 15% of a salt of a lower molecular weight organic acid selected from the group consisting of alkali metal, ammonium and alkanol ammonium salts of formic, acetic, propionic, butyric, and valeric acids, and mixtures thereof, and (ii) from about 1.0 to about 25% of an alcohol having the formula ROH wherein R is an alkyl having one to six carbon atoms, and (d) from about 10 to about 90% water.
Claim 3
Dependent Claim
26 words
The composition of claim 1 wherein the carrier is selected from the group consisting of sodium chloride, sodium sulfate, calcium sulfate, glycerol, and combinations of same.
Claim 4
Dependent Claim
22 words
The composition of claims 1 or 2 wherein the alkanol ammonium is selected from the group consisting of monoethanolammonium, diethanolammonium and triethanolammonium.
Claim 5
Dependent Claim
17 words
The composition of claims 1 or 2 wherein the pH is from about 6 to about 10.
Claim 6
Dependent Claim
19 words
The composition of claims 4 wherein the percent by weight of water is from about 40% to about 90%.
Claim 7
Dependent Claim
20 words
The composition of claim 5 wherein the percent by weight of the enzyme is from about 0.006% to about 2.5%.
Claim 8
Dependent Claim
20 words
The composition of claim 6 wherein the percent by weight of the alcohol is from about 4% to about 10%.
Claim 9
Dependent Claim
20 words
The composition of claim 7 wherein the percent by weight of the surfactant is from about 5% to about 30%.
Claim 10
Dependent Claim
20 words
The composition of claim 8 wherein the percent by weight of the salt is from about 2% to about 6%.
Claim 11
Dependent Claim
15 words
The composition of claim 9 wherein the pH is from about 6 to about 9.
Claim 12
Dependent Claim
17 words
The composition of claim 10 wherein the enzyme is a proteolytic enzyme obtained from the Bacillus Substilis.
Claim 13
Dependent Claim
14 words
The composition of claim 11 wherein the salt is sodium acetate or sodium propionate.
Claim 14
Dependent Claim
10 words
The composition of claim 12 wherein the alcohol is ethanol.
Claim 15
Dependent Claim
42 words
The composition of claim 13 wherein the percent by weight of the surfactant is about 30%; and wherein the surfactant is a mixture comprised of about 33.3% by weight of an anionic surfactant and about 66.6% by weight of a nonionic surfactant.
Claim 16
Dependent Claim
42 words
The composition of claim 13 wherein the nonionic surfactant is an ethoxylated fatty alcohol having the formula RO--(CH 2 CH 2 O) n H wher R is from 8 to 18 carbon atoms and n is an integer from 1 to 500.
Claim 17
Dependent Claim
81 words
The composition of claim 15 wherein the nonionic surfactant is a mixture of: a. the condensation product of 1 mole of an aliphatic alcohol having from 12 to 13 carbon atoms in either a straight or branched chain configuration, with an average of 6.5 moles of ethylene oxide; and b. the condensation product of 1 mole of an aliphatic alcohol, having from 12 to 15 carbon atoms in either a straight or branched chain configuration, with 9 moles of ethylene oxide.
Claim 18
Dependent Claim
28 words
The composition of claim 16 wherein the nonionic surfactant mixture is comprised of about 50% by weight of component a and about 50% by weight of component b.
Claim 19
Dependent Claim
46 words
The composition of claim 17 further comprising by weight of the composition: a. from about 1% to about 10% of a solubilizing compound; and b. from about 1% to about 75% of an isoparaffinic mixture of petroleum distallates having an average molecular weight of about 154.
Claim 20
Dependent Claim
42 words
The composition of claim 18 wherein the solubilizing agent is about 3%-6% by weight of the composition; wherein the isoparaffinic mixture of petroleum distallates is about 10%-40% by weight of the total composition and wherein the solubilizing agent is sodium xylene sulfonate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1988-05-20 | 1988-11-19 | 1989-05-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-20 | 1992-11-19 | 1993-05-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-20 | 1996-11-19 | 1997-05-21 | $7700.00 | Window Closed / Lapsed |