US4517241A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chromatographic support material
Assignee: Individual
•
Filing: 1982-12-02
•
Granted: 1985-05-14
•
Expires: 2002-12-02
Abstract
Abstract A support material comprised of a substrate and a coating bound to the substrate made up of a polymer containing succinimide units or derivatives thereof. A method for preparing a support material comprising reacting a polymer containing succinimide units with surface amine groups on a substrate.
Inventors
- 👤 Andrew J. Alpert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 07:37
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/02; B01J20/06; B01J20/08; B01J20/10; B01J20/103; B01J20/22; B01J20/26; B01J20/262; B01J20/264; B01J20/265; B01J20/28; B01J20/28002; B01J20/28004; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3276; B01J20/328; C; C12; C12N; C12N9/00; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10T; Y10T428/00; Y10T428/26; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996; Y10T428/2998; Y10T428/31504; Y10T428/31551; Y10T428/31623; Y10T428/31645; Y10T428/31649; Y10T428/31652; Y10T428/31667; Y10T428/31721; Y10T428/8305
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
40 words
A coated support material comprising a chromatographically suitable substrate; and an uniform immobilized coating, susceptible to derivatization to a form suitable for protein chromatography, on said substrate formed by the reaction of a polymer containing succinimide units with said substrate.
Claim 2
Dependent Claim
40 words
A coated support material comprising a chromatographically suitable substrate; and an uniform immobilized coating, susceptible to derivatization to a form suitable for protein chromatography, on said substrate formed by the reaction of a polymer containing succinimide units with said substrate.
Claim 3
Dependent Claim
23 words
The support material of claim 1 wherein succinimide units of said immobilized polymer are converted to aspartyl residues thereby producing a hydrophilic coating.
Claim 4
Dependent Claim
17 words
The support material of claim 1 wherein succinimide units of said immobilized polymer are converted to aspartamides.
Claim 5
Dependent Claim
13 words
The support material of claim 2 or 3 wherein said polymer is polysuccinimide.
Claim 6
Dependent Claim
16 words
The support material of claim 2 or 3 wherein said polymer is a derivative of polysuccinimide.
Claim 7
Dependent Claim
17 words
The support material of claim 2 or 3 wherein said polymer is a copolymer containing succinimide units.
Claim 8
Dependent Claim
28 words
The support material of claim 2 or 3 wherein said substrate is a porous inorganic support material selected from the group consisting of glass, alumina, silica and titania.
Claim 9
Dependent Claim
21 words
The support material of claim 7 wherein said substrate consists of particles of about 5 to about 10 microns in diameter.
Claim 10
Dependent Claim
23 words
The support material of claim 1 wherein said substrate is an organic material chosen from the group consisting of polystyrene, polyacrylates and polymethacrylates.
Claim 11
Dependent Claim
36 words
A coated support material comprising a chromatographically suitable substrate; and a uniform immobilized coating, susceptible to derivatization to a form suitable for protein chromatography, on said substrate formed by the reaction of polysuccinimide with said substrate.
Claim 12
Dependent Claim
36 words
A coated support material comprising a chromatographically suitable substrate; and a uniform immobilized coating, susceptible to derivatization to a form suitable for protein chromatography, on said substrate formed by the reaction of polysuccinimide with said substrate.
Claim 13
Dependent Claim
23 words
The support material of claim 10 wherein succinimide unit of the immobilized polysuccinimides are converted to aspartyl residues thereby producing a hydrophilic coating.
Claim 14
Dependent Claim
17 words
The support material of claim 10 wherein succinimide units of the immobilized polysuccinimide are converted to aspartamides.
Claim 15
Dependent Claim
28 words
The support material of claim 11 or 12 wherein said substrate is a porous inorganic support material selected from the group consisting of glass, alumina, silica and titania.
Claim 16
Dependent Claim
21 words
The support material of claim 13 wherein said substrate consists of particles of about 5 to about 10 microns in diameter.
Claim 17
Dependent Claim
25 words
The support material of claim 11 or 12 wherein said substrate is an organic material chosen from the group consisting of polystyrene, polyacrylates and polymethacrylates.
Claim 18
Dependent Claim
40 words
A coated support material comprising a chromatographically suitable inorganic substrate material; and an immobilized coating on said substrate formed by the reaction of polysuccinimide with said substrate and the subsequent conversion of succinimide units of the polysuccinimide to aspartyl residues.
Claim 19
Dependent Claim
40 words
A coated support material comprising a chromatographically suitable inorganic substrate material; and an immobilized coating on said substrate formed by the reaction of polysuccinimide with said substrate and the subsequent conversion of succinimide units of the polysuccinimide to aspartyl residues.
Claim 20
Dependent Claim
22 words
The support material of claim 8 wherein said substrate material is selected from the group consisting of glass, alumina, silica and titania.
Claim 21
Dependent Claim
21 words
The support material of claim 8 wherein said substrate consists of particles of about 5 to about 10 microns in diameter.
Claim 22
Independent Claim
81 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines or both; contacting said substrate material with a polymer having succinimide units under conditions which promote the reaction of said polymer with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 23
Independent Claim
81 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines or both; contacting said substrate material with a polymer having succinimide units under conditions which promote the reaction of said polymer with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 24
Dependent Claim
10 words
The method of claim 19 wherein said polymer is polysuccinimide.
Claim 25
Dependent Claim
13 words
The method of claim 19 wherein said polymer is a derivative of polysuccinimide.
Claim 26
Dependent Claim
14 words
The method of claim 19 wherein said polymer is a copolymer containing succinimide units.
Claim 27
Dependent Claim
24 words
The method of claim 19 which further comprises converting said succinimide units in the immobilized polymer to aspartyl residues thereby producing a hydrophilic coating.
Claim 28
Dependent Claim
28 words
The method of claim 19 which further comprises reacting succinimide units in the immobilized polymer with a substance containing one or more primary or secondary amine functional groups.
Claim 29
Dependent Claim
21 words
The method of claim 19 which further comprises reacting succinimide units in the immobilized polymer to introduce phosphonate and sulfonate groups.
Claim 30
Dependent Claim
24 words
The method of claim 19 wherein said substrate material is an inorganic substrate provided from the group consisting of silica, glass, alumina and titania.
Claim 31
Dependent Claim
17 words
The method of claim 19 wherein said polymer is immobilized on said substrate in a batchwise preparation.
Claim 32
Dependent Claim
21 words
The process of claim 19 wherein said substrate material is packed into a column and reacted in situ with said polymer.
Claim 33
Independent Claim
72 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material; and contacting said substrate material with a polymer having succinimide units and having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 34
Independent Claim
72 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material; and contacting said substrate material with a polymer having succinimide units and having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 35
Independent Claim
79 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatographically suitable substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines, or both; and contacting said substrate material with polysuccinimide under conditions which promote the reaction of said polysuccinimide with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 36
Independent Claim
79 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatographically suitable substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines, or both; and contacting said substrate material with polysuccinimide under conditions which promote the reaction of said polysuccinimide with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 37
Dependent Claim
24 words
The method of claim 30 wherein said substrate material is an inorganic substrate provided from the group consisting of silica, glass, alumina and titania.
Claim 38
Dependent Claim
23 words
The method of claim 31 which further comprises converting succinimide units of said immobilized polysuccinimide to aspartyl residues thereby producing a hydrophilic coating.
Claim 39
Dependent Claim
28 words
The method of claim 31 which further comprises reacting succinimide units of said immobilized polysuccinimide with a substance containing one or more primary or secondary amine functional groups.
Claim 40
Independent Claim
19 words
The method of claims 32 or 33 wherein said polymer is immobilized on said substrate in a batchwise preparation.
Claim 41
Independent Claim
23 words
The method of claims 32 or 33 wherein said substrate material is packed into a column and reacted in situ with said polymer.
Claim 42
Independent Claim
68 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material; and contacting said substrate material with a polysuccinimide having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 43
Independent Claim
68 words
A method for producing a coating on a substrate, said method comprising: providing a chromatographically suitable substrate material; and contacting said substrate material with a polysuccinimide having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography.
Claim 44
Dependent Claim
23 words
The method of claim 36 which further comprises converting succinimide units in said immobilized polysuccinimide to aspartyl residues thereby producing a hydrophilic coating.
Claim 45
Dependent Claim
28 words
The method of claim 36 which further comprises reacting succinimide units of said immobilized polysuccinimide with a substance containing one or more primary or secondary amine functional groups.
Claim 46
Independent Claim
90 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatagraphically suitable inorganic substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines or both; contacting said substrate material with polysuccinimide under conditions which promote the reaction of said polymer with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography; and converting succinimide units in the immobilized polysuccinimide to aspartyl residues.
Claim 47
Independent Claim
90 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatagraphically suitable inorganic substrate material having surface amine functional groups, said amine groups being primary amines, secondary amines or both; contacting said substrate material with polysuccinimide under conditions which promote the reaction of said polymer with said amine groups of said substrate thereby immobilizing said polymer on said substrate and forming a uniform coating susceptible to derivatization to a form suitable for protein chromatography; and converting succinimide units in the immobilized polysuccinimide to aspartyl residues.
Claim 48
Dependent Claim
22 words
The method of claim 39 wherein said inorganic substrate material is provided from the group consisting of silica, glass, alumina and titania.
Claim 49
Dependent Claim
17 words
The method of claim 39 wherein said polymer is immobilized on said substrate in a batchwise preparation.
Claim 50
Dependent Claim
21 words
The process of claim 39 wherein said substrate material is packed into a column and reacted in situ with said polymer.
Claim 51
Independent Claim
78 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatographically suitable inorganic substrate material; contacting said substrate material with polysuccinimide having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography; and converting succinimide units of said polysuccinimide to aspartyl residues.
Claim 52
Independent Claim
78 words
A method for producing a hydrophilic coating on a substrate, said method comprising: providing a chromatographically suitable inorganic substrate material; contacting said substrate material with polysuccinimide having functional groups capable of reacting with said substrate material under conditions which promote the reaction of said functional groups with said substrate material so as to form an uniform immobilized coating susceptible to derivatization to a form suitable for protein chromatography; and converting succinimide units of said polysuccinimide to aspartyl residues.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-13 | 1988-11-12 | 1989-05-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-13 | 1992-11-12 | 1993-05-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-13 | 1996-11-12 | 1997-05-14 | $7700.00 | Window Closed / Lapsed |