US4514499A
Expired / Lapsed
US
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Cell culture using a monolithic support
Assignee: CHARLES RIVER BIOTECHNICAL SERVICES Inc A CORP OF
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Filing: 1983-02-04
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Granted: 1985-04-30
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Expires: 2003-02-04
Abstract
Abstract A novel immobilized cell composite used in a novel apparatus/method for cell culture. The composite comprises: (a) a high surface area monolithic support having a multiplicity of mutually parallel channels passing therethrough, the channels having walls formed of a medium-insoluble, non-toxic, composition and the support having at least about 20 channels per square inch of cross-sectional area; and (b) a population of plant or animal tissue cells anchored to the porous channel walls.
Inventors
- 👤 Lee A. Noll
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:27
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12M; C12M23/00; C12M23/02; C12M23/06; C12M25/00; C12M25/06; C12M25/10; C12M33/00; C12M33/12
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
68 words
An immobilized cell composite comprising: (a) a high surface area monolithic support having an inlet and an outlet end, a multiplicity of mutually parallel channels passing therethrough, the channels having walls formed of a medium-insoluble, non-toxic, composition and the support having at least about 20 channels per square inch of cross-sectional area; and (b) a population of plant or animal tissue cells anchored to the porous channel walls.
Claim 2
Dependent Claim
68 words
An immobilized cell composite comprising: (a) a high surface area monolithic support having an inlet and an outlet end, a multiplicity of mutually parallel channels passing therethrough, the channels having walls formed of a medium-insoluble, non-toxic, composition and the support having at least about 20 channels per square inch of cross-sectional area; and (b) a population of plant or animal tissue cells anchored to the porous channel walls.
Claim 3
Dependent Claim
31 words
A composite in accordance with claim 1 wherein the dimensions of the channels and channel walls are such that the support contains about 100-1000 channels per square inch of cross-sectional area.
Claim 4
Dependent Claim
20 words
A composite in accordance with claim 1 wherein the support has an aspect ratio in the range of about 1:100.
Claim 5
Dependent Claim
18 words
A composite in accordance with claim 2 wherein the support contains at least 400 channels per square inch.
Claim 6
Dependent Claim
15 words
A composite in accordance with claim 4 wherein the channels have a square cross-sectional area.
Claim 7
Dependent Claim
36 words
A composite in accordance with claim 1 wherein the channel walls are formed of a sintered, porous ceramic material selected from the group consisting of cordierite, alumina, silica, titania, mullite, zircon spodumene, mica, and combinations thereof.
Claim 8
Dependent Claim
14 words
A composite in accordance with claim 6 wherein the porous ceramic material is cordierite.
Claim 9
Dependent Claim
16 words
A composite in accordance with claim 1 wherein the cell population is derived from plant tissue.
Claim 10
Dependent Claim
16 words
A composite in accordance with claim 1 wherein the cell population is derived from animal tissue.
Claim 11
Dependent Claim
18 words
A composite in accordance with claim 9 wherein the cell population is a lymphocyte, epithelial or fibroblast type.
Claim 12
Dependent Claim
13 words
A composite in accordance with claim 10 wherein the tissue source is human.
Claim 13
Dependent Claim
79 words
A process for culturing plant or animal tissue cells wherein the cells are anchored to a monolithic medium-insoluble, non-toxic support and a cell nutrient medium is flowed past the cells, characterized in that (a) the support consists of a monolith having a multiplicity of parallel channels passing therethrough and an inlet and outlet end; (b) the channels have walls suitable for cell anchoring; and (c) the support has at least about 20 channels per square inch of cross-sectional area.
Claim 14
Dependent Claim
79 words
A process for culturing plant or animal tissue cells wherein the cells are anchored to a monolithic medium-insoluble, non-toxic support and a cell nutrient medium is flowed past the cells, characterized in that (a) the support consists of a monolith having a multiplicity of parallel channels passing therethrough and an inlet and outlet end; (b) the channels have walls suitable for cell anchoring; and (c) the support has at least about 20 channels per square inch of cross-sectional area.
Claim 15
Dependent Claim
32 words
The process recited in claim 12 wherein the cell nutrient medium is constantly monitored for nutrient and metabolite levels and adjustments are constantly made to the medium to optimize the nutrient levels.
Claim 16
Dependent Claim
29 words
The process recited in claim 13 wherein the monitored and optimized nutrient levels are the pH of the medium, dissolved oxygen level, dissolved carbon dioxide level, and glucose concentration.
Claim 17
Dependent Claim
33 words
A process in accordance with claim 12 wherein the channel walls are formed of a composition selected from the group consisting of cordierite, alumina, silica, titania, mullite, zircon, spodumene, mica, and combinations thereof.
Claim 18
Dependent Claim
22 words
A process in accordance with claim 15 wherein the support has at least about 100-1000 channels per square inch of cross-sectional area.
Claim 19
Dependent Claim
18 words
A process in accordance with claim 16 wherein the cell population is of lymphocyte, epithelial, or fibroblast type.
Claim 20
Dependent Claim
25 words
A process in accordance with claim 16 wherein the medium is flowed through the channels at a linear flow velocity not exceeding about 36 cm/min.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-29 | 1988-10-29 | 1989-04-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-29 | 1992-10-29 | 1993-04-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-29 | 1996-10-29 | 1997-04-30 | $7700.00 | Window Closed / Lapsed |