US4513085A
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Functionally independent cloning vectors for use in streptomyces
Assignee: Eli Lilly and Co
•
Filing: 1982-10-07
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Granted: 1985-04-23
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Expires: 2002-10-07
Abstract
Abstract The present invention discloses functionally independent selectable recombinant DNA cloning vectors for use in Streptomyces.
Inventors
- 👤 Walter M. Nakatsukasa
- 👤 James A. Mabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:31
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76
Claims Analysis
Total Claims: 50
Claim 1
Independent Claim
52 words
A functionally independent recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL7 and (b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 2
Dependent Claim
52 words
A functionally independent recombinant DNA cloning vector comprising: (a) a functional origin of replication-containing restriction fragment of plasmid pEL7 and (b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell, said host cell being susceptible to transformation, cell division, and culture.
Claim 3
Dependent Claim
19 words
The cloning vector of claim 1 wherein the restriction fragment of pEL7 is the ˜6.78 kb BclI restriction fragment.
Claim 4
Dependent Claim
15 words
The cloning vector of claim 1 wherein one DNA segment confers antibiotic resistance to thiostrepton.
Claim 5
Dependent Claim
15 words
The cloning vector of claim 1 wherein one DNA segment confers antibiotic resistance to neomycin.
Claim 6
Dependent Claim
15 words
The cloning vector of claim 1 wherein one DNA segment confers antibiotic resistance to erythromycin.
Claim 7
Dependent Claim
24 words
The cloning vector of claim 1 wherein one DNA segment is the ˜1.6 kb BamHI or ˜0.8 kb BclI restriction fragment of plasmid pLR2.
Claim 8
Dependent Claim
20 words
The cloning vector of claim 1 wherein one DNA segment is the ˜3.4 kb BamHI restriction fragment of plasmid pLR1.
Claim 9
Dependent Claim
43 words
The cloning vector of claim 1 wherein one DNA segment is selected from the group consisting of the ˜2.8 kb SalI, ˜2.7 kb SalI-BglII, ˜3.0 kb HindIII, ˜2.5 kb SalI-BamHI, ˜2.8 kb XhoI-BglII, and the ˜4.1 kb EcoRI-BamHI restriction fragments of plasmid pIJ43.
Claim 10
Dependent Claim
44 words
The functionally independent recombinant DNA cloning vector of claim 1 which is selected from the group consisting of plasmids pNM702A, pNM702B, pNM703A, pNM703B, pNM704A, pNM704B, pNM705A, pNM705B, pNM706A, pNM706B, pNM707A, pNM707B, pNM709A, pNM709B, pNM710A, pNM710B, pNM711A, pNM711B, pNM712A, pNM712B, pNM719A, pNM719B, pNM720A and pNM720B.
Claim 11
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM702A.
Claim 12
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM702B.
Claim 13
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM703A.
Claim 14
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM703B.
Claim 15
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM704A.
Claim 16
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM704B.
Claim 17
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM705A.
Claim 18
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM705B.
Claim 19
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM706A.
Claim 20
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM706B.
Claim 21
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM707A.
Claim 22
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM707B.
Claim 23
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM709.
Claim 24
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM710A.
Claim 25
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM712A.
Claim 26
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM719B.
Claim 27
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 9 which is pNM720A.
Claim 28
Dependent Claim
38 words
A recombinant DNA cloning vector which comprises a replicon that is functional in E. coli, a DNA segment that confers antibiotic resistance in E. coli and a restriction fragment comprising a recombinant DNA cloning vector of claim 1.
Claim 29
Dependent Claim
48 words
The recombinant DNA cloning vector of claim 27 in which the replicon that is functional in E. coli and the DNA segment that confers antibiotic resistance in E. coli comprise a restriction fragment of a plasmid selected from the group consisting of plasmids pBR322, pBR324, pBR325 and pBR328.
Claim 30
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 28 which is pNM708A.
Claim 31
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 28 which is pNM713A.
Claim 32
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 28 which is pNM714A.
Claim 33
Dependent Claim
11 words
The recombinant DNA cloning vector of claim 28 which is pNM717A.
Claim 34
Dependent Claim
15 words
A transformed restrictionless Streptomyces host cell comprising a recombinant DNA cloning vector of claim 1.
Claim 35
Dependent Claim
15 words
A transformed restrictionless Streptomyces host cell comprising a recombinant DNA cloning vector of claim 9.
Claim 36
Dependent Claim
32 words
The transformed host cell of claim 34 which is selected from the group consisting of Streptomyces ambofaciens, Streptomyces aureofaciens, Streptomyces griseofuscus, Streptomyces fradiae, Streptomyces lividans, Streptomyces granuloruber, Streptomyces tenebrarius, and Streptomyces cinnamonensis.
Claim 37
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pNM702A.
Claim 38
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pNM705A.
Claim 39
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pNM707A.
Claim 40
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pNM703A.
Claim 41
Dependent Claim
12 words
The transformed host cell of claim 35 which is Streptomyces lividans 1326/pNM703A.
Claim 42
Dependent Claim
11 words
The transferred host cell of claim 35 which is Streptomyces ambofaciens/pNM719A.
Claim 43
Dependent Claim
11 words
The transformed host cell of claim 35 which is Streptomyces ambofaciens/pNM720A.
Claim 44
Dependent Claim
27 words
A transformed host cell selected from the group consisting of restrictionless Streptomyces and E. coli, said hose cell comprising a recombinant DNA cloning vector of claim 27.
Claim 45
Dependent Claim
27 words
A transformed host cell selected from the group consisting of restrictionless Streptomyces and E. coli, said host cell comprising a recombinant DNA cloning vector of claim 28.
Claim 46
Dependent Claim
11 words
The transformed host cell of claim 43 which is E. coli.
Claim 47
Dependent Claim
13 words
The transformed host cell of claim 44 which is E. coli K12 HB101/pNM708A.
Claim 48
Dependent Claim
13 words
The transformed host cell of claim 44 which is E. coli K12 HB101/pNM713A.
Claim 49
Dependent Claim
13 words
The transformed host cell of claim 44 which is E. coli K12 HB101/pNM717A.
Claim 50
Dependent Claim
9 words
The 6.78 kb BclI restriction fragment of claim 2.
References Cited (Backward Citations)
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