US4512763A
Expired / Lapsed
US
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Method and apparatus for selective removal of constituents of blood
Assignee: Gamma Medical Products Inc
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Filing: 1981-05-04
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Granted: 1985-04-23
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Expires: 2001-05-04
Abstract
Abstract A method for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprises the steps of: removing blood from the patient; separating the constituent containing component from the rest of the patient's blood; preparing an antibody; contacting the constituent containing component with the antibody so that the antibody may adsorb the constituent from the component; recombining the antibody contacted constituent containing component with the other components of the removed blood; and injecting the combined components of blood into the patient; wherein the adsorbing antibody is prepared by the steps of: removing selected cells from the constituent containing component of the patient's blood; injecting the removed cells into a non-human animal; allowing the non-human animal to manufacture antibodies to the injected cells; removing blood from the non-human animal; separating the same constituent containing component from the removed blood of the non-human animal; and removing the antibody from the constituent containing component of the non-human animal's blood. Another method for making the adsorbent antibody includes the steps of: creating cell cultures; cloning the antibody producing cells; removing large quantities of the antibody; and purifying the antibody. General and specific apparatus for effecting the above methods are also disclosed.
Inventors
- 👤 Barbara Schneider
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:25
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61M; A61M1/00; A61M1/36; A61M1/3687
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
186 words
A method of removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising the steps of: (a) removing a volume of the patient's blood from a vein; (b) separating the constituent containing component from the removed blood; (c) preparing an antibody; (d) contacting the patient's component with the antibody so that the antibody may adsorb the appropriate constituent from the component; (e) recombining the antibody contacted component with the other components of the patient's blood; and (f) injecting the combined components into a vein of the patient; wherein the antibody is prepared by the steps of: (g) removing a volume of the patient's blood; (h) separating the component from the removed blood; (i) removing selected cells from the component; (j) injecting the removed cells into a non-human animal such as a goat, rabbit or mouse; (k) delaying sufficient time for the non-human animal to manufacture antibodies to the injected cells; (l) removing blood from the non-human animal; (m) separating the same component from the removed non-human animal's blood; and (n) removing the antibodies from the non-human animal's component.
Claim 2
Independent Claim
186 words
A method of removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising the steps of: (a) removing a volume of the patient's blood from a vein; (b) separating the constituent containing component from the removed blood; (c) preparing an antibody; (d) contacting the patient's component with the antibody so that the antibody may adsorb the appropriate constituent from the component; (e) recombining the antibody contacted component with the other components of the patient's blood; and (f) injecting the combined components into a vein of the patient; wherein the antibody is prepared by the steps of: (g) removing a volume of the patient's blood; (h) separating the component from the removed blood; (i) removing selected cells from the component; (j) injecting the removed cells into a non-human animal such as a goat, rabbit or mouse; (k) delaying sufficient time for the non-human animal to manufacture antibodies to the injected cells; (l) removing blood from the non-human animal; (m) separating the same component from the removed non-human animal's blood; and (n) removing the antibodies from the non-human animal's component.
Claim 3
Independent Claim
173 words
A method of removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising the steps of: (a) removing a volume of the patient's blood from a vein; (b) separating the constituent containing component from the removed blood; (c) preparing an antibody; (d) contacting the patient's component with the antibody so that the antibody may adsorb the appropriate constituent from the component; (e) recombining the antibody contacted component with the other components of the patient's blood; and (f) injecting the combined components into a vein of the patient; wherein the antibody is made by the stpes of: (g) removing a volume of the patient's blood; (h) separating the component from the removed blood; (i) removing selected cells from the component; (j) creating cultures of these cells grown in nutrient media and testing these cells for the presence of the antibody; (k) cloning the created cells; (l) heating of cells to remove large quantities of portions of the antibody; and (m) purifying the portion of the antibody.
Claim 4
Independent Claim
173 words
A method of removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising the steps of: (a) removing a volume of the patient's blood from a vein; (b) separating the constituent containing component from the removed blood; (c) preparing an antibody; (d) contacting the patient's component with the antibody so that the antibody may adsorb the appropriate constituent from the component; (e) recombining the antibody contacted component with the other components of the patient's blood; and (f) injecting the combined components into a vein of the patient; wherein the antibody is made by the stpes of: (g) removing a volume of the patient's blood; (h) separating the component from the removed blood; (i) removing selected cells from the component; (j) creating cultures of these cells grown in nutrient media and testing these cells for the presence of the antibody; (k) cloning the created cells; (l) heating of cells to remove large quantities of portions of the antibody; and (m) purifying the portion of the antibody.
Claim 5
Dependent Claim
98 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component, wherein said antibody is fixed relative to said contacting means; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting thhe re-combined components into a vein of the patient.
Claim 6
Dependent Claim
98 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component, wherein said antibody is fixed relative to said contacting means; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting thhe re-combined components into a vein of the patient.
Claim 7
Dependent Claim
64 words
The apparatus of claim 3 wherein said contacting means includes: a matrix; and means for spreading the blood component in a generally horizontal direction to contact said matrix at the upper end thereof, whereby the blood component will flow downwardly in contact with said matrix to the lower end thereof; and means for collecting the blood component from the lower end of said matrix.
Claim 8
Dependent Claim
13 words
The apparatus of claim 4 wherein said contacting means includes partially digested nylon.
Claim 9
Dependent Claim
21 words
The apparatus of claim 3 wherein said blood volume removing means operates at a rate of from 30 to 60 cc/minute.
Claim 10
Dependent Claim
163 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting the re-combined components into a vein of the patient; wherein said component contacting means further comprises: an input plate structure for receiving the constituent containing component from the separating means and including a plurality of appertures; an output plate structure for providing the antibody contacted component to said recombining means and also including a similar plurality of apertures; and a plurality of fibers for interconnecting corresponding appertures located on said input and output plate structures and including said constituent adsorbing antibody covalently bound thereto.
Claim 11
Dependent Claim
163 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting the re-combined components into a vein of the patient; wherein said component contacting means further comprises: an input plate structure for receiving the constituent containing component from the separating means and including a plurality of appertures; an output plate structure for providing the antibody contacted component to said recombining means and also including a similar plurality of apertures; and a plurality of fibers for interconnecting corresponding appertures located on said input and output plate structures and including said constituent adsorbing antibody covalently bound thereto.
Claim 12
Dependent Claim
12 words
The apparatus of claim 7 wherein said fibers are in parallel arrangement.
Claim 13
Dependent Claim
28 words
The apparatus of claim 7 wherein said constituent containing component remains in contact with said fibers due to the natural surface tension between the component and the fibers.
Claim 14
Dependent Claim
28 words
The apparatus of claim 7 wherein the received constituent containing component flows onto the ends of said fibers at the input plate structure under the influence of gravity.
Claim 15
Dependent Claim
30 words
The apparatus of claim 7 wherein the diameter of said input and output plate structures is approximately 5 cm and wherein the length of said fibers is approximately 12.5 cm.
Claim 16
Dependent Claim
17 words
The apparatus of claim 7 wherein each plate structure includes approximately one apperture for each ten fibers.
Claim 17
Dependent Claim
22 words
The apparatus of claim 7 wherein said fibers have a diameter of approximately 0.05 mm and a length of approximately 12.5 cm.
Claim 18
Dependent Claim
19 words
The apparatus of claim 7 wherein said adsorbent antibody on said fibers has a thickness of approximately 0.05 microns.
Claim 19
Dependent Claim
27 words
The apparatus of claim 7 wherein said constituent is a specific lymphocyte subset; said fibers are made of carbon; and said adsorbent antibody is a monoclonal antibody.
Claim 20
Dependent Claim
12 words
The apparatus of claim 7 wherein said fibers are made of nylon.
Claim 21
Dependent Claim
27 words
The apparatus of claim 7 wherein said constituent is a specific lymphocyte subset; said fibers are made of nylon; and said adsorbent antibody is a human immunoglobulin.
Claim 22
Dependent Claim
57 words
The apparatus of claim 7 wherein said plate structures have a diameter of approximately 5 cm; said fibers are made of partially digested nylon and have a diameter of approximately 0.05 mm and a length of approximately 12.5 cm; and wherein said adsorbent antibody is an immunoglobulin or other reactant such as rheumatoid factor or Fc receptor.
Claim 23
Dependent Claim
99 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting the re-combined components into a vein of the patient; wherein said contacting means further comprises strips or sheets of nylon.
Claim 24
Dependent Claim
99 words
Apparatus for removing a constituent such as immunoglobulins, lymphocytes, or immune complexes from a component of a patient's blood comprising: means for removing a volume of the patient's blood from a vein; means for separating the constituent containing component from the removed blood; means for contacting the patient's component with an antibody capable of adsorbing said constituent from the component; means for recombining the antibody contacted component with the other components of the patient's blood; and means for injecting the re-combined components into a vein of the patient; wherein said contacting means further comprises strips or sheets of nylon.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-22 | 1988-10-22 | 1989-04-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-22 | 1992-10-22 | 1993-04-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-22 | 1996-10-22 | 1997-04-23 | $7700.00 | Window Closed / Lapsed |