US4510251A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescent polarization assay for ligands using aminomethylfluorescein derivatives as tracers
Assignee: Abbott Laboratories
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Filing: 1982-11-08
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Granted: 1985-04-09
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Expires: 2002-11-08
Abstract
Abstract This disclosure relates to a method for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine, wherein a novel class of aminomethylfluorescein derivative tracer compounds are employed as reagents in fluorescence polarization immunoassays.
Inventors
- 👤 Curtis L. Kirkemo
- 👤 Mohammed T. Shipchandler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:37
CPC Classifications
C; C07; C07D; C07D493/00; C07D493/02; C07D493/10; C07J; C07J41/00; C07J41/0005; C07J41/0016; C09; C09B; C09B11/00; C09B11/04; C09B11/10; C09B11/24; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
112 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: ##STR14## wherein R 1 is a ##STR15## group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R 2 is hydrogen or a group represented by R 1 ; and R 3 is hydrogen, amino or carboxyl; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 2
Independent Claim
112 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: ##STR14## wherein R 1 is a ##STR15## group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R 2 is hydrogen or a group represented by R 1 ; and R 3 is hydrogen, amino or carboxyl; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 3
Dependent Claim
11 words
A method according to claim 1 wherein R 2 is hydrogen.
Claim 4
Dependent Claim
19 words
A method according to claim 2 wherein R" has a molecular weight within a range of 50 to 4000.
Claim 5
Dependent Claim
15 words
A method according to claim 3 wherein R" is a cortisol-analog of the formula: ##STR16##
Claim 6
Dependent Claim
15 words
A method according to claim 3 wherein R" is an estriol-analog of the formula: ##STR17##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-08 | 1988-10-08 | 1989-04-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-08 | 1992-10-08 | 1993-04-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-08 | 1996-10-08 | 1997-04-09 | $7700.00 | Window Closed / Lapsed |