US4508738A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Non-sucrose fermenting Pedioccus pentosaceus
Assignee: Microlife Technics Inc
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Filing: 1982-03-17
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Granted: 1985-04-02
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Expires: 2002-03-17
Abstract
Abstract Non-sucrose fermenting strains of Pediococcus pentosaceus are described. The non-sucrose fermenting Pediococcus pentosaceus strains have been cured to remove a single, natural plasmid which has been found to control the fermentation of sucrose to produce lactic acid. The natural plasmid removed is between about 30 to 35 megadaltons in molecular weight. Compositions including the non-sucrose fermenting strains of Pediococcus pentosaceus are useful for food fermentations, particularly meat fermentations.
Inventors
- 👤 Carlos F. Gonzalez
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 13:58
CPC Classifications
A; A23; A23B; A23B4/00; A23B4/12; A23B7/00; A23B7/10; A23L; A23L13/00; A23L13/40; A23L13/45; C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/746; C12R; C12R2001/00; C12R2001/01; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
82 words
A storage stable biologically pure strain of Pediococcus pentosaceus adapted for food fermentations which strain is unable to ferment sucrose to lactic acid as a result of curing removal of a single naturally occurring plasmid from a known strain of Pediococcus pentosaceus, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which strain with the plasmid removed is able to ferment dextrose to lactic acid in a food.
Claim 2
Dependent Claim
82 words
A storage stable biologically pure strain of Pediococcus pentosaceus adapted for food fermentations which strain is unable to ferment sucrose to lactic acid as a result of curing removal of a single naturally occurring plasmid from a known strain of Pediococcus pentosaceus, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which strain with the plasmid removed is able to ferment dextrose to lactic acid in a food.
Claim 3
Dependent Claim
17 words
The strain of claim 1 as a composition containing at least about 10 6 cells per ml.
Claim 4
Dependent Claim
19 words
The strain of claim 1 wherein the known strain from which the plasmid is removed is Pediococcus pentosaceus NRRL-B-11,465.
Claim 5
Dependent Claim
89 words
A composition which comprises a biologically pure culture of a Pediococcus pentosaceus which culture is unable to ferment sucrose to lactic acid as a result of curing removal of a naturally occurring plasmid measuring about 30 to 35 megadaltons in molecular weight from a known strain of Pediococcus pentosaceus containing only this plasmid, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which culture is able to ferment dextrose to lactic acid in a food.
Claim 6
Dependent Claim
89 words
A composition which comprises a biologically pure culture of a Pediococcus pentosaceus which culture is unable to ferment sucrose to lactic acid as a result of curing removal of a naturally occurring plasmid measuring about 30 to 35 megadaltons in molecular weight from a known strain of Pediococcus pentosaceus containing only this plasmid, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which culture is able to ferment dextrose to lactic acid in a food.
Claim 7
Dependent Claim
94 words
A composition which comprises a biologically pure culture of a Pediococcus pentosaceus which culture is unable to ferment sucrose to lactic acid as a result of curing removal of a naturally occurring plasmid measuring between about 30 to 35 megadaltons from a known strain of Pediococcus pentosaceus containing only this plasmid, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which culture is able to ferment dextrose to lactic acid in a food and a preservation agent for the culture.
Claim 8
Dependent Claim
94 words
A composition which comprises a biologically pure culture of a Pediococcus pentosaceus which culture is unable to ferment sucrose to lactic acid as a result of curing removal of a naturally occurring plasmid measuring between about 30 to 35 megadaltons from a known strain of Pediococcus pentosaceus containing only this plasmid, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, and which culture is able to ferment dextrose to lactic acid in a food and a preservation agent for the culture.
Claim 9
Dependent Claim
24 words
The composition of claim 5 wherein the preservation agent is a freezing stabilizing agent which maintains viable cells and wherein the composition is frozen.
Claim 10
Dependent Claim
12 words
The strain of claim 1 which is Pediococcus pentosaceus NRRL-B-15007 or NRRL-B-15019.
Claim 11
Dependent Claim
106 words
In the method for preparation of fermented foods by providing a culture in the foods and then fermenting the foods the improvement which comprises: providing in the foods a strain of Pediococcus pentosaceus which is unable to ferment sucrose to lactic acid as a result of curing removal of a single naturally occurring plasmid from a known strain of Pediococcus pentosaceus, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, wherein the fermentation is conducted in the presence of dextrose which is fermented to produce lactic acid which lowers pH of the food.
Claim 12
Dependent Claim
106 words
In the method for preparation of fermented foods by providing a culture in the foods and then fermenting the foods the improvement which comprises: providing in the foods a strain of Pediococcus pentosaceus which is unable to ferment sucrose to lactic acid as a result of curing removal of a single naturally occurring plasmid from a known strain of Pediococcus pentosaceus, wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465, wherein the fermentation is conducted in the presence of dextrose which is fermented to produce lactic acid which lowers pH of the food.
Claim 13
Dependent Claim
23 words
The method of claim 8 wherein the Pediococcus pentosaceus provided in the food is NRRL-B-15007 and wherein the food is a fermented meat.
Claim 14
Dependent Claim
25 words
The method of claim 8 wherein the culture provided in the food is a biologically pure strain of Pediococcus pentosaceus which does not ferment sucrose.
Claim 15
Dependent Claim
61 words
The method of claim 8 wherein the Pediococcus pentosaceus which is unable to ferment sucrose to lactic acid has been cured to remove the plasmid by contact with a sub-lethal concentration of a curing agent or at curing temperature or a combination thereof, separation of non-sucrose fermenting strains and concentration of the strains prior to providing the strain in the food.
Claim 16
Dependent Claim
17 words
The method of claim 11 wherein the curing agent is acridine orange, ethidium bromide, acriflavin or proflavin.
Claim 17
Dependent Claim
17 words
The method of claim 11 wherein the curing is at elevated temperatures no higher than 50° C.
Claim 18
Independent Claim
100 words
A method of removing a single plasmid from a known strain of Pediococcus pentosaceus having the ability to ferment sucrose to lactic acid with the plasmid which comprises contacting viable known strain cells of Pediococcus pentosaceus with a sub-lethal curative concentration of a plasmid curing agent or exposure to curing temperatures wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465 and the strain with the plasmid removed is unable to ferment sucrose to lactic acid but is able to ferment dextrose to lactic acid in a food.
Claim 19
Independent Claim
100 words
A method of removing a single plasmid from a known strain of Pediococcus pentosaceus having the ability to ferment sucrose to lactic acid with the plasmid which comprises contacting viable known strain cells of Pediococcus pentosaceus with a sub-lethal curative concentration of a plasmid curing agent or exposure to curing temperatures wherein the single plasmid removed corresponds in size and in control of sucrose fermentation to a single plasmid in Pediococcus pentosaceus NRRL-B-11,465 and the strain with the plasmid removed is unable to ferment sucrose to lactic acid but is able to ferment dextrose to lactic acid in a food.
Claim 20
Dependent Claim
17 words
The method of claim 14 wherein the curing agent is acridine orange, ethidium bromide, acriflavin or proflavin.
Claim 21
Dependent Claim
16 words
The method of claim 14 wherein Pediococcus pentosaceus NRRL-B-15007 or NRRL-B-15019 is produced without the plasmid.
Claim 22
Dependent Claim
17 words
The method of claim 14 wherein the curing is at elevated temperatures no higher than 50° C.
Claim 23
Dependent Claim
19 words
The method of claim 8 wherein the known strain from which the plasmid is removed is Pediococcus pentosaceus NRRL-B-11465.
Claim 24
Dependent Claim
24 words
The method of claim 18 wherein removal of the plasmid produces a strain which has the fermentation characteristics of Pediococcus pentosaceus NRRL-B-15007 or NRRL-B-15019.
Claim 25
Dependent Claim
18 words
The composition of claim 4 in admixture with a metal ion which stimulates growth of the modified strain.
Claim 26
Dependent Claim
12 words
The composition of claim 20 wherein the metal ion is manganese ion.
Claim 27
Dependent Claim
29 words
The method of claim 8 wherein the strain which is unable to ferment sucrose is in admixture with a metal ion which stimulates the growth of the modified strain.
Claim 28
Dependent Claim
13 words
The method of claim 22 wherein the metal ion is a manganese ion.
References Cited (Backward Citations)
US4248513A
Cited
US4303679A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-01 | 1988-10-01 | 1989-04-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-01 | 1992-10-01 | 1993-04-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-01 | 1996-10-01 | 1997-04-02 | $7700.00 | Window Closed / Lapsed |