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US4493533A Expired / Lapsed US ⏱️ 0.0 years remaining

Immersion oil containing aliphatic thio compounds

Assignee: F Hoffmann La Roche AG
•
Filing: 1982-04-20
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Granted: 1985-01-15
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Expires: 2002-04-20
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Abstract

Abstract Immersion oils containing aliphatic thio compounds are described. The oils have optical properties and are useful in immersion optics such as fluorescence microscopy.

Inventors

  • 👤 Martin Petrzilka
  • 👤 Inge Pracht
  • 👤 Urs Ruf

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-28 02:25
CPC Classifications
C; C07; C07C; C07C323/00

Claims Analysis

Total Claims: 18
Claim 1
Independent Claim 184 words
In the combination for immersion oil optics of an immersion oil completely filling the space between the objective lens and a preparation or a cover glass over the preparation to be optically investigated, the improvement wherein the optical immersion oil comprises one or more aliphatic thio compounds of the formula R.sup.1 O--(CH.sub.2).sub.n.sbsb.1 --S--(CH.sub.2).sub.m.sbsb.1 --OR.sup.2I wherein R 1 denotes hydrogen, benzoyl or a group --(CH 2 ) n .sbsb.2 --OR 3 and R 2 denotes hydrogen or a group --(CH 2 ) m .sbsb.2 --OR 4 , R 3 represents hydrogen or a group --(CH 2 ) n .sbsb.3 --OH and R 4 represents hydrogen or a group --(CH 2 ) m .sbsb.3 --OH, the indices n 1 , m 1 , n 2 , m 2 , n 3 and m 3 signify whole numbers of 2 to 6 and the sums n 1 +m 1 or n 2 +m 2 or n 3 +m 3 amount to at most 8, or derivatives thereof in which one or more of the methylene groups is/are substituted by alkyl groups containing 1 to 4 carbon atoms.
Claim 2
Dependent Claim 184 words
In the combination for immersion oil optics of an immersion oil completely filling the space between the objective lens and a preparation or a cover glass over the preparation to be optically investigated, the improvement wherein the optical immersion oil comprises one or more aliphatic thio compounds of the formula R.sup.1 O--(CH.sub.2).sub.n.sbsb.1 --S--(CH.sub.2).sub.m.sbsb.1 --OR.sup.2I wherein R 1 denotes hydrogen, benzoyl or a group --(CH 2 ) n .sbsb.2 --OR 3 and R 2 denotes hydrogen or a group --(CH 2 ) m .sbsb.2 --OR 4 , R 3 represents hydrogen or a group --(CH 2 ) n .sbsb.3 --OH and R 4 represents hydrogen or a group --(CH 2 ) m .sbsb.3 --OH, the indices n 1 , m 1 , n 2 , m 2 , n 3 and m 3 signify whole numbers of 2 to 6 and the sums n 1 +m 1 or n 2 +m 2 or n 3 +m 3 amount to at most 8, or derivatives thereof in which one or more of the methylene groups is/are substituted by alkyl groups containing 1 to 4 carbon atoms.
Claim 3
Dependent Claim 43 words
In the improved oil according to claim 1 the immersion oil wherein R 1 represents hydrogen or a group --(CH 2 ) n .sbsb.2 --OR 3 , wherein n and R 3 are as defined in claim 1, said immersion oil being water-soluble.
Claim 4
Dependent Claim 22 words
In the improved oil according to claim 1 the immersion oil wherein R 1 represents benzoyl, said immersion oil being non water-soluble.
Claim 5
Dependent Claim 31 words
In the improved combination according to claim 1 the immersion oil wherein the refractive index n D of the immersion oil at 23° C. amounts to about 1.500 to about 1.525.
Claim 6
Dependent Claim 24 words
In the improved combination according to claim 4 the immersion oil wherein the refractive index n D at 23° C. amounts to about 1.515.
Claim 7
Dependent Claim 19 words
In the improved combination according to claim 1 the immersion oil wherein R 3 and R 4 represent hydrogen.
Claim 8
Dependent Claim 43 words
In the improved combination according to claim 1 the immersion oil wherein R 1 represents hydrogen, benzoyl or a group --(CH 2 ) n .sbsb.2 --OR 3 and R 2 and R 3 represent hydrogen and n 2 is as defined claim 1.
Claim 9
Dependent Claim 23 words
In the improved combination according to claim 7 the immersion oil wherein R 1 represents hydrogen or benzoyl and R 2 represents hydrogen.
Claim 10
Dependent Claim 33 words
In the improved combination according to claim 1 the immersion oil wherein the sums n 1 +m 1 or n 2 +m 2 or n 3 +m 3 amount to at most 6.
Claim 11
Dependent Claim 23 words
In the improved combination according to claim 1 the immersion oil wherein the compound of formula I contains at most two alkyl groups.
Claim 12
Dependent Claim 18 words
In the improved combination according to claim 1 the immersion oil wherein the alkyl groups are methyl groups.
Claim 13
Dependent Claim 42 words
In the improved combination according to claim 1 the immersion oil wherein R 1 denotes hydrogen or benzoyl, R 2 signifies hydrogen and the sum n 1 +m 1 amounts to 4 to 6 and the alkyl groups are methyl or dimethyl.
Claim 14
Dependent Claim 16 words
In the improved combination according to claim 12 the immersion oil comprising 2,2'-thiodiethanol or 2-[(2-hydroxyethyl)thio]ethyl benzoate.
Claim 15
Dependent Claim 25 words
In the improved combination according to claim 1 further the immersion oil comprising additives for increasing or decreasing the refractive index of the immersion oil.
Claim 16
Dependent Claim 31 words
In the improved combination according to claim 14 the immersion oil wherein the additives are selected from the group consisting of aliphatic glycols and alkanols containing 1 to 20 carbon atoms.
Claim 17
Dependent Claim 20 words
In the improved combination according to claim 15 the immersion oil comprising 2,2'-thiodiethanol and polyethyleneglycol, said immersion oil being water-soluble.
Claim 18
Dependent Claim 22 words
In the improved combination according to claim 15 the immersion oil comprising 2-[(2-hydroxyethyl)thio]-ethyl benzoate and isophytol, said immersion oil being non water-soluble.

References Cited (Backward Citations)

GB487323A Cited
GB647911A Cited
US2582605A Cited
FR1285749A Cited
US3297393A Cited
US3437402A Cited
FR1575898A Cited
JPS4834723A Cited
US3876288A Cited
US3929667A Cited
JPS5141595A Cited
US4108794A Cited
US3979301A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1988-01-15 1988-07-16 1989-01-15 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1992-01-15 1992-07-16 1993-01-15 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1996-01-15 1996-07-16 1997-01-15 $7700.00 Window Closed / Lapsed

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