US4491632A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing antibodies to hepatitis virus and cell lines therefor
Assignee: General Hospital Corp
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Filing: 1983-07-26
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Granted: 1985-01-01
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Expires: 2003-07-26
Abstract
Abstract Cell lines for producing monoclonal antibodies to hepatitis virus are established by immunizing animal lymphocytes with hepatitis antigen to form antibody producing cells which then are fused with myeloma cells. The resultant somatic cell hybrids can be cloned. These clones produce monoclonal antibodies to individual antigenic determinates unique to hepatitis virus.
Inventors
- 👤 Jack R. Wands
- 👤 Vincent R. Zurawski, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:09
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/42; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
52 words
A composition comprising a hybrid continuous cell line that produces antibody to a viral hepatitis antigen which comprises a cell hybrid of an animal spleen cell immunized with viral hepatitis antigen fused to a myeloma derived from the same animal species as the spleen cell and a culture medium for said hybrid.
Claim 2
Dependent Claim
52 words
A composition comprising a hybrid continuous cell line that produces antibody to a viral hepatitis antigen which comprises a cell hybrid of an animal spleen cell immunized with viral hepatitis antigen fused to a myeloma derived from the same animal species as the spleen cell and a culture medium for said hybrid.
Claim 3
Dependent Claim
16 words
The composition of claim 1 wherein said hybrid is located in a culture medium containing hypoxanthine-aminopterinthymidine.
Claim 4
Dependent Claim
17 words
The composition of any one of claims 1 or 2 wherein said antibody is an IgG fraction.
Claim 5
Dependent Claim
17 words
The composition of any one of claims 1 or 2 wherein said antibody is an IgM fraction.
Claim 6
Dependent Claim
17 words
The composition of any one of claims 1 or 2 wherein said antibody is an IgA fraction.
Claim 7
Dependent Claim
11 words
The composition of claim 1 wherein said animal is a mouse.
Claim 8
Dependent Claim
16 words
The composition of claim 1 wherein said viral hepatitis antigen is a human viral hepatitis antigen.
Claim 9
Dependent Claim
46 words
A composition comprising a hybrid continuous cell line that produces antibody to a human viral hepatitis antigen which comprises a cell hybrid of a BALB/c mouse spleen cell immunized with human viral hepatitis antigen fused to a mouse myeloma and a culture medium for said hybrid.
Claim 10
Dependent Claim
46 words
A composition comprising a hybrid continuous cell line that produces antibody to a human viral hepatitis antigen which comprises a cell hybrid of a BALB/c mouse spleen cell immunized with human viral hepatitis antigen fused to a mouse myeloma and a culture medium for said hybrid.
Claim 11
Dependent Claim
16 words
The composition of claim 8 wherein said hybrid is located in a culture medium containing hypoxanthineaminopterinthymidine.
Claim 12
Dependent Claim
17 words
The composition of any one of claims 8 or 9 wherein said antibody is an IgG fraction.
Claim 13
Dependent Claim
16 words
The composition of any of claims 8 or 9 wherein said antibody is an IgM fraction.
Claim 14
Dependent Claim
17 words
The composition of any one of claims 8 or 9 wherein said antibody is an IgA fraction.
Claim 15
Dependent Claim
53 words
A hybrid continuous cell line that procuces antibody to viral hepatitis antigen which comprises a cell hybrid of an animal spleen cell immunized with viral hepatitis antigen fused to a myeloma derived from the same animal species as the spleen cell and having the identifying characteristics of ATCC ascession number CRL-8017 or CRL-8018.
Claim 16
Dependent Claim
53 words
A hybrid continuous cell line that procuces antibody to viral hepatitis antigen which comprises a cell hybrid of an animal spleen cell immunized with viral hepatitis antigen fused to a myeloma derived from the same animal species as the spleen cell and having the identifying characteristics of ATCC ascession number CRL-8017 or CRL-8018.
References Cited (Backward Citations)
US4196265A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-01 | 1988-07-02 | 1989-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-01 | 1992-07-02 | 1993-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-01 | 1996-07-02 | 1997-01-01 | $7700.00 | Window Closed / Lapsed |