US4491533A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immersion oil for fluorescence microscopy
Assignee: RP Cargille Laboratories Inc
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Filing: 1983-09-08
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Granted: 1985-01-01
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Expires: 2003-09-08
Abstract
Abstract A heavy mineral oil having a relatively high viscosity is employed as an immersion oil for use in fluorescence microscopy. The oil exhibits no auto fluorescent characteristics while exhibiting a compatible refractive index to allow the same to be used with condenser/objective lens systems in fluorescence microscopy.
Inventors
- 👤 Robert L. Sacher
- 👤 William J. Sacher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 23:23
CPC Classifications
G; G02; G02B; G02B21/00; G02B21/33; G02B21/16
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
89 words
In a process for using a fluorescent microscope for studying materials which fluoresce in which process an immersion oil is employed in conjunction with the objective lens the improvement therewith of using a heavy mineral oil having a refractive index of about 1.480 as the immersion oil in said process, with said oil having a density between 0.875 to 0.905 and which oil exhibits substantially little background flourescence when illuminated by near ultraviolet light with said oil further having a viscosity of greater than 150 centistokes at 25° C.
Claim 2
Dependent Claim
89 words
In a process for using a fluorescent microscope for studying materials which fluoresce in which process an immersion oil is employed in conjunction with the objective lens the improvement therewith of using a heavy mineral oil having a refractive index of about 1.480 as the immersion oil in said process, with said oil having a density between 0.875 to 0.905 and which oil exhibits substantially little background flourescence when illuminated by near ultraviolet light with said oil further having a viscosity of greater than 150 centistokes at 25° C.
Claim 3
Dependent Claim
23 words
The process according to claim 1 wherein said oil has a density of 0.88 with a viscosity at 25° C. of 162 centistokes.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-01-01 | 1988-07-02 | 1989-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-01-01 | 1992-07-02 | 1993-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-01-01 | 1996-07-02 | 1997-01-01 | $7700.00 | Window Closed / Lapsed |