US4487838A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Radio-labelled cross-linking reagents
Assignee: Massachusetts Institute of Technology
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Filing: 1982-01-12
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Granted: 1984-12-11
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Expires: 2002-01-12
Abstract
Abstract A crosslinking reagent consisting of a first functional group capable of forming a covalent derivative with a material of interest; a second functional group capable of forming an in situ bond to a neighboring material upon activation; a cleavable bond separating said first and second groups; and a radioactive marker to identify the neighboring material after cleavage of said bond, whereby in situ protein interactions can be studied.
Inventors
- 👤 Richard O. Hynes
- 👤 Martin A. Schwartz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 05:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/534
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
71 words
A crosslinking reagent comprising; (a) a first functional group capable of forming a covalent derivative with a material of interest; (b) a second functional group capable of forming an in situ bond to a neighboring material upon photolysis, (c) a chemically cleavable bond separating said first and second groups; and (d) label means for labelling the neighboring material after cleavage of said bond, whereby in situ protein interactions can be studied.
Claim 2
Dependent Claim
71 words
A crosslinking reagent comprising; (a) a first functional group capable of forming a covalent derivative with a material of interest; (b) a second functional group capable of forming an in situ bond to a neighboring material upon photolysis, (c) a chemically cleavable bond separating said first and second groups; and (d) label means for labelling the neighboring material after cleavage of said bond, whereby in situ protein interactions can be studied.
Claim 3
Dependent Claim
13 words
The reagent of claim 1 wherein said first functional group is an ester.
Claim 4
Dependent Claim
12 words
The reagent of claim 2 wherein said ester is a succinimide ester.
Claim 5
Dependent Claim
21 words
The reagent of claim 1 wherein said second functional group is a group capable of forming a reactive nitrene upon photolysis.
Claim 6
Dependent Claim
23 words
The reagent of claim 4 wherein the second functional group is at least one member of the group of alkylazides, arylazides and aralkylazides.
Claim 7
Dependent Claim
13 words
The reagent of claim 5 wherein the second functional group is a phenylazide.
Claim 8
Dependent Claim
13 words
The reagent of claim 1 wherein the cleavable bond is a disulfide bond.
Claim 9
Dependent Claim
22 words
The reagent of claim 1 wherein the label means is a radioactive marker which remains with the second functional group upon cleavage.
Claim 10
Dependent Claim
37 words
The reagent of claim 1, 2, 3, 4, 5 or 6 wherein the cleavable bond is a disulfide bond and the label means is a radioisotope of sulfur which remains with the second functional group upon cleavage.
Claim 11
Dependent Claim
14 words
A reagent having the following formula: ##STR2## where S is a radioisotope of sulfur.
Claim 12
Dependent Claim
14 words
A reagent having the following formula: ##STR2## where S is a radioisotope of sulfur.
Claim 13
Dependent Claim
94 words
A method of analyzing interactions between biological materials in situ, the method comprising: (a) binding the first functional group of the cross-linking reagent of claim 1 to a material of interest in vitro; (b) introducing the crosslinking reagent and the bound material of interest into a study situs; (c) exposing the situs to light to activate the second functional group and the reagent and bind neighboring materials to the crosslinking reagent; (d) lysing the situs; (e) reducing the reagent; (f) isolating the labelled materials; and (g) analyzing the reduced, isolated materials for chemical composition.
References Cited (Backward Citations)
US3940420A
Cited
DE2917001A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-11 | 1988-06-11 | 1988-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-11 | 1992-06-11 | 1992-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-11 | 1996-06-11 | 1996-12-11 | $7700.00 | Window Closed / Lapsed |