Detection of malignant tumor cells

Claim 2

A process for detecting the presence of cancerous or malignant tumor cells in a cell collection, said cells comprising a cancer RECOGNIN, comprising applying to said cell collection a specific monoclonal anti-cancer RECOGNIN antibody, which attaches to cancerous cells and can thereby be detected by visible or signal-emitting means attached to said antibody, said cancer RECOGNIN being derived from cancerous tumor tissue or cells, and characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave length of 280 mμand a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.

Claim 3

The process according to claim 1 wherein said cell collection is in vivo.

Claim 4

The process according to claim 1 wherein said cell collection is in vitro.

Claim 5

The process according to claim 1 wherein the cancer RECOGNIN is MALIGNIN.

Claim 6

A composition comprising monoclonal anti-MALIGNIN antibody-FAST or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN; and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280μ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 7

A composition comprising monoclonal anti-MALIGNIN antibody-FAST or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN; and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280μ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 8

A composition comprising monoclonal anit-MALIGNIN antibody-SLOW or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN; and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280 mμ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 9

A composition comprising monoclonal anit-MALIGNIN antibody-SLOW or a purified fraction thereof, whereby said antibody or a purified fraction thereof attaches to cancerous cells, said cells comprising MALIGNIN; and can thereby be detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at an alkaline pH, and has a spectrophotometric absorption peak wave length of 280 mμ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 10

A composition comprising monoclonal anti-MALIGNIN antibody-FAST and SLOW or a purified fraction thereof, whereby said antibody is cytotoxic to and kills said cancer cells, said cells comprising MALIGNIN, whereby said antibody or a purified fraction thereof attaches to cancerous cells and can thereby to detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitive precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at a alkaline pH, and has an spectrophotometric absorption peak wave length of 280 mμ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 11

A composition comprising monoclonal anti-MALIGNIN antibody-FAST and SLOW or a purified fraction thereof, whereby said antibody is cytotoxic to and kills said cancer cells, said cells comprising MALIGNIN, whereby said antibody or a purified fraction thereof attaches to cancerous cells and can thereby to detected by visible or signal-emitting means attached to said antibody, said MALIGNIN being derived from brain tumor cells, and which forms a single line precipitate with its specific antibody in quantitive precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solution having an acid or neutral pH, and insoluble at a alkaline pH, and has an spectrophotometric absorption peak wave length of 280 mμ, a molecular weight of about 10,000, and an amino acid composition approximately as follows: ______________________________________ APPROXIMATE NO. OF RESIDUES ______________________________________ Aspartic Acid 9 Threonine 5 Serine 5 Glutamic Acid 13 Proline 4 Glycine 6 Alanine 7 Valine 6 1/2 Cysteine 1 Methionine 2 Isoleucine 4 Leucine 8 Tyrosine 3 Phenylalanine 3 Lysine 6 Histidine 2 Arginine 5 89 ______________________________________ ammonia and the amino acids cysteic, hydroxyproline, norleucine, isodesmosine, lysinonorleucine and gamma-aminobutyric acid being absent in detectable amounts.

Claim 12

A composition comprising a mixture of the antibody according to claim 5, monoclonal anti-MALIGNIN antibody-FAST or a purified fraction thereof and the antibody according to claim 6, monoclonal anti-MALIGNIN antibody-SLOW or a purified fraction thereof, whereby said mixture is cytotoxic to and kills cancerous cells.

Claim 13

The product according to claim 5 wherein said monoclonal anti-MALIGNIN antibody-FAST is complexed with any substance which it is desired to have preferentially attached to cancerous cells.

Claim 14

The product according to claim 6 wherein said monoclonal anti-MALIGNIN antibody-SLOW is complexed with any substance which it is desired to have preferentially attached to cancerous cells.

Claim 15

The product according to claim 7 wherein said monoclonal anti-MALIGNIN antibody-FAST/SLOW is complexed with any substance which it is desired to have preferentially attached to cancer cells.

Claim 16

A composition comprising monoclonal anti-cancer RECOGNIN antibody whereby said anti-cancer RECOGNIN antibody is produced by the injection into an organism or collection of cells of a purified cancer RECOGNIN said cancer RECOGNIN having the ability to induce or cause the cells of said organism or collection to produce specific anti-cancer RECOGNIN antibody, wherein such anti-cancer RECOGNIN antibody producing cells are treated to make them self-perpetuating and identified by their production of monoclonal anti-cancer RECOGNIN antibody, said cancer RECOGNIN being derived from cancerous tumor tissue or cells, and characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave length of 280 mμ and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.

Claim 17

A composition comprising monoclonal anti-cancer RECOGNIN antibody whereby said anti-cancer RECOGNIN antibody is produced by the injection into an organism or collection of cells of a purified cancer RECOGNIN said cancer RECOGNIN having the ability to induce or cause the cells of said organism or collection to produce specific anti-cancer RECOGNIN antibody, wherein such anti-cancer RECOGNIN antibody producing cells are treated to make them self-perpetuating and identified by their production of monoclonal anti-cancer RECOGNIN antibody, said cancer RECOGNIN being derived from cancerous tumor tissue or cells, and characterized by forming a single line precipitate with its specific antibody in quantitative precipitin tests and Ouchterlony gel diffusion tests, being soluble in water and aqueous solutions having an acid or neutral pH, and insoluble at alkaline pH, having a spectrophotometric absorption peak wave length of 280 mμ and a molecular weight of from about 3,000 to about 25,000, and further characterized by having an amino acid residue composition characterized by high proportions of glutamic and aspartic acids and high ratios of glutamic and aspartic acids to histidine.

Claim 18

The product according to claim 12 comprising a monoclonal anti-cancer RECOGNIN antibody or a purified fraction thereof which is cytotoxic to and kills cancerous cells.

Claim 19

The composition according to claim 12 further comprising a complex of said monoclonal anti-cancer RECOGNIN antibody or a purified fraction thereof and any substance which it is desired to have attached to cancerous cells.

Claim 20

The composition according to claim 12 further comprising visible or signal-emitting means attached to said antibody or a purified fraction thereof whereby cancer cells are detectable by said means when said antibody is attached to said cells.

Claim 21

The composition of claim 12 wherein said antibody-producing cells are hybridized to make them self-perpetuating.

Claim 22

The composition of claim 16 wherein said cells are the cells of a murine organism.

Claim 23

The process according to claim 1 wherein said antibody or antibody fraction is derived by a process comprising priming antibody-producing cells with a purified cancer RECOGNIN.

Claim 24

The process according to claim 18 wherein said antibody is hybridomally produced by a process comprising priming murine spleen cells with a purified cancer RECOGNIN.

Claim 25

The composition of claims 5, 6 or 7 wherein said antibody or antibody fraction is derived by a process comprising priming antibody-producing cells with purified MALIGNIN.

Claim 26

The composition of claim 20 wherein said antibody or antibody fraction is hybridomally-produced by a process comprising priming murine spleen cells.