Bone marrow transplant method and apparatus

Claim 2

In bone marrow transplant apparatus wherein a pair of aspiration needles are insertable into the bone of a mammal in closely-spaced relation to one another, one of said aspiration needles being connectable to a bone marrow collector and the other of said aspiration needles being connectable to an intravenous solution source, the combination therewith comprising: a first chamber interposed between said one aspiration needle and said bone marrow collector to normally establish fluid communication therebetween; a second chamber interposed between said second aspiration needle and said intravenous solution source to normally establish fluid communication therebetween; fluid flow control means associated with said first and second chambers to regulate the pressure level within each of said chambers such that the pressure level in said first chamber is variable between a negative pressure to induce the removal of bone marrow from the bone marrow site into said chamber and a positive pressure level operative to force the bone marrow recovered into said bone marrow collector and the pressure level in said second chamber being variable between a negative pressure level to induce the flow of intravenous solution from said intravenous solution source into said second chamber and a positive pressure level to cause said intravenous solution to flow from said second chamber through said second aspiration needle into the bone marrow site; and valve means associated with said fluid flow control means being selectively movable between open and closed positions to regulate the flow of fluid and materials to and from said first and second chambers.

Claim 3

In apparatus according to claim 1 including first delivery lines extending from fluid communication with said first chamber to said one aspiration needle and said intravenous solution source and second delivery lines extending from said second chamber to each of said bone marrow collector and said intravenous solution source.

Claim 4

In apparatus according to claim 2, said chambers and said delivery lines being in the form of a unitary disposable assembly removably positioned with respect to said fluid flow control means.

Claim 5

In apparatus according to claim 3, said valve means being associated with each of said delivery lines to regulate the flow of material therethrough.

Claim 6

In apparatus according to claim 4, each of said delivery lines being defined by a flexible conduit, said valve means each defined by a pinch valve engageable with a flexible conduit.

Claim 7

In apparatus according to claim 1, including a second solution source comprised of an intravenous solution and an anti-coagulant.

Claim 8

In apparatus according to claim 3, the concentration of anti-coagulant in said second solution source corresponding to that in said first solution source.

Claim 9

In bone marrow transplant apparatus wherein a pair of aspiration needles are insertable into the bone of a living mammal in closely spaced relation to one another, one of said aspiration needles being connectable to a bone marrow collection bag and the other of said aspiration needles being connectable to an intravenous solution source containing a mixture of intravenous solution and an anti-coagulant, the combination therewith comprising: a first syringe interposed between said one aspiration needle and said bone marrow collection bag to normally establish fluid communication therebetween including first delivery lines extending from fluid communication with said first syringe to said one aspiration needle and said intravenous solution source; a second syringe interposed between said second aspiration needle and said intravenous solution source to normally establish fluid communication therebetween including second delivery lines extending from said second syringe to each of said bone marrow collection bag and said intravenous solution source; fluid flow control means associated with said first and second syringes to regulate the pressure level within each of said syringes such that the pressure level in said first syringe is variable between a negative pressure to induce the removal of bone marrow from the bone marrow site into said syringe and a positive pressure level operative to force the bone marrow recovered into said bone marrow collection bag and the pressure level in said second syringe being variable between a negative pressure level to induce the flow of intravenous solution from said intravenous solution source into said second syringe and a positive pressure level to cause said intravenous solution to flow from said second syringe through said second aspiration needle into the bone marrow site; and valve means engageable with said first and second delivery lines being selectively movable between open and closed positions to regulate the flow of fluid and materials to and from said first and second syringes.

Claim 10

In bone marrow transplant apparatus wherein a pair of aspiration needles are insertable into the bone of a living mammal in closely spaced relation to one another, one of said aspiration needles being connectable to a bone marrow collection bag and the other of said aspiration needles being connectable to an intravenous solution source containing a mixture of intravenous solution and an anti-coagulant, the combination therewith comprising: a first syringe interposed between said one aspiration needle and said bone marrow collection bag to normally establish fluid communication therebetween including first delivery lines extending from fluid communication with said first syringe to said one aspiration needle and said intravenous solution source; a second syringe interposed between said second aspiration needle and said intravenous solution source to normally establish fluid communication therebetween including second delivery lines extending from said second syringe to each of said bone marrow collection bag and said intravenous solution source; fluid flow control means associated with said first and second syringes to regulate the pressure level within each of said syringes such that the pressure level in said first syringe is variable between a negative pressure to induce the removal of bone marrow from the bone marrow site into said syringe and a positive pressure level operative to force the bone marrow recovered into said bone marrow collection bag and the pressure level in said second syringe being variable between a negative pressure level to induce the flow of intravenous solution from said intravenous solution source into said second syringe and a positive pressure level to cause said intravenous solution to flow from said second syringe through said second aspiration needle into the bone marrow site; and valve means engageable with said first and second delivery lines being selectively movable between open and closed positions to regulate the flow of fluid and materials to and from said first and second syringes.

Claim 11

In apparatus according to claim 8, including a second solution source comprised of a mixture of an intravenous solution and anti-coagulant connectable to one of said second delivery lines.

Claim 12

In apparatus according to claim 8, said fluid flow control means including a common slide member supporting said syringes in side-by-side oppositely facing relation, said slide member having plunger-engaging members thereon, and plunger means associated with each of said syringes being simultaneously actuatable by said plunger-engaging elements to increase the pressure in one of said syringes while reducing the pressure in the other of said syringes.

Claim 15

The method according to claim 11 characterized by injecting the intravenous solution simultaneously with the withdrawal of bone marrow from the bone marrow site.

Claim 16

The method according to claim 11 including the step of mixing the bone marrow and sinusoidal blood with a mixture of an intravenous solution and an anti-coagulant.