US4476049A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the extraction of immune interferon
Assignee: Hoffmann La Roche Inc
•
Filing: 1983-09-20
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Granted: 1984-10-09
•
Expires: 2003-09-20
Abstract
Abstract A method for the extraction of intact recombinant human immune interferon with guanidine-HCl is disclosed. This method permits the purification to homogenity of intact recombinant human immune interferon.
Inventors
- 👤 Hsiang-Fu Kung
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 09:31
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/57; C07K1/00; C07K1/107; C07K1/113; C07K1/1136; C07K16/00; C07K16/18; C07K16/24; C07K16/249; Y; Y10; Y10S; Y10S435/00; Y10S435/811; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/808; Y10S530/809
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
24 words
A method for extracting intact recombinant human immune interferon from transformed microorganisms containing this protein comprising extracting said transformed microorganisms with a protease inhibitor.
Claim 2
Independent Claim
24 words
A method for extracting intact recombinant human immune interferon from transformed microorganisms containing this protein comprising extracting said transformed microorganisms with a protease inhibitor.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the protease inhibitor is guanidine-HCl.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the transformed microorganisms are suspended in a solution of guanidine-HCl.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein the solution is at least about 4M guanidine-HCl.
Claim 6
Dependent Claim
22 words
The method of claim 1 wherein the intact mature recombinant human immune interferon protein has a molecular weight of about 18,000 daltons.
Claim 7
Dependent Claim
27 words
The method of claim 1 wherein the transformed microorganisms preparation is separated after being treated with the protease inhibitor into a supernate fraction and cell debris fraction.
Claim 8
Dependent Claim
11 words
The method of claim 6, wherein the protease inhibitor is guanidine-HCl.
References Cited (Backward Citations)
US3981991A
Cited
US4017600A
Cited
US4357422A
Cited
US4414150A
Cited
US4382027A
Cited
US4388234A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-09 | 1988-04-09 | 1988-10-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-09 | 1992-04-09 | 1992-10-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-09 | 1996-04-09 | 1996-10-09 | $7700.00 | Window Closed / Lapsed |