US4473652A
Expired / Lapsed
US
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Method and immunochemical measurement
Assignee: Fujifilm Holdings Corp
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Filing: 1983-06-22
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Granted: 1984-09-25
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Expires: 2003-06-22
Abstract
Abstract A method for immunochemical assay of an antigen or antibody by labelling the antigen or antibody with a specific cyanine or merocyanine dye containing a carboxy group followed by effecting an immune reaction and photochemical processing thereof is provided. The amount of the antigen or antibody is measured in term of optical density of developed silver halide which is brought into contact with either the antigen-antibody reaction product or the unreacted material. This immunochemical assay method gives high detection sensitivity in a simple operation manner.
Inventors
- 👤 Masaki Okazaki
- 👤 Nobuhito Masuda
- 👤 Yoshiro Kumano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/583
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
89 words
A method for immunochemical measurement of an antigen or antibody which comprises labelling an antigen or antibody with a labelling substance, said labelling substance being a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye.
Claim 2
Independent Claim
89 words
A method for immunochemical measurement of an antigen or antibody which comprises labelling an antigen or antibody with a labelling substance, said labelling substance being a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye.
Claim 3
Independent Claim
142 words
A method for immunochemically measuring an antigen or antibody which comprises: labelling an antigen or antibody with a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye, subjecting the thus obtained dye-labelled antigen or antibody to an immune reaction, bringing either the bound antibody or antigen or the free antigen or antibody into contact with silver halide after or at the same time as B/F separation, exposing the resulting product to light which the corresponding dye absorbs, developing the exposed silver halide, and, measuring the resulting optical density.
Claim 4
Independent Claim
142 words
A method for immunochemically measuring an antigen or antibody which comprises: labelling an antigen or antibody with a cyanine or merocyanine dye comprising a carboxy-containing substituent on a heterocyclic nucleus of said dye, wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine color-forming moiety of the terminal heterocyclic nucleus of said cyanine dye and wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye, subjecting the thus obtained dye-labelled antigen or antibody to an immune reaction, bringing either the bound antibody or antigen or the free antigen or antibody into contact with silver halide after or at the same time as B/F separation, exposing the resulting product to light which the corresponding dye absorbs, developing the exposed silver halide, and, measuring the resulting optical density.
Claim 5
Dependent Claim
34 words
The method of claim 2 wherein said cyanine dye comprises a carboxy-containing substituent bound to an atom other than the nitrogen atom in the cyanine-color-forming moiety of the terminal heterocyclic nucleus of said dye.
Claim 6
Dependent Claim
132 words
The method of claim 1 or 2 wherein said cyanine dye has the formula: ##STR8## wherein ma and na each represents 1 or 2 and may be the same or different; pa represents 2 or 3; qa represents 1 or 2; L 1 , L 2 and L 3 , which each may be the same or different represents a methine group optionally substituted with a lower alkyl group, an aryl group or a halogen atom; Z a and Z 1a , which may be the same or different, each represents the non-metallic atomic group necessary to complete a 5- or 6-membered nitrogen-containing heterocyclic nucleus; X represents an inorganic or organic anion; R a and R 1a each represents an unsubstituted or substituted alkyl group; and R 2a represents a carboxy-containing substituent.
Claim 7
Dependent Claim
21 words
The method of claim 2 wherein said merocyanine dye comprises a carboxy-containing substituent on the heterocyclic nucleus of said merocyanine dye.
Claim 8
Dependent Claim
78 words
The method of claim 5 wherein said merocyanine dye is represented by formula: ##STR9## wherein pb represents an integer of 2 or 3; nb represents an integer of 1 or 2; Z b represents the non-metallic atomic group necessary to complete a 5- or 6-membered nitrogen containing heterocyclic basic nucleus; Q b represents the non-metallic atom group necessary to complete a 5- or 6-membered nitrogen-containing acidic heterocyclic nucleus; and R b represents an unsubstituted or substituted alkyl group.
Claim 9
Dependent Claim
83 words
The method of claim 2, 3, or 4, wherein said carboxy-containing substituent is represented by formula: --P.sub.i --Qa.sub.j --COOH wherein P represents ##STR10## --CO--, --O-- or --S-- wherein R 4 represents a hydrogen atom, an unsubstituted or substituted alkyl group; Qa represents an unsubstituted or substituted alkylene group, an unsubstituted or substituted arylene group, an aralkylene group, an alkarylene group, a dipeptide residue or a tripeptide residue; and i and j each represents 0 or 1 which may be the same or different.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1987-09-25 | 1988-03-26 | 1988-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-25 | 1992-03-26 | 1992-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-25 | 1996-03-26 | 1996-09-25 | $7700.00 | Window Closed / Lapsed |