Process for producing a vasal fibrinolytic active principle and the enzymatic product so prepared

Claim 1

Process for the production of a vasal fibrinolytic active principle called angiokinase enzyme, starting from richly vascularized mammalian connective tissue, said process comprising defatting, drying and pulverizing said tissue to a powder; subjecting said powder to pancreatin lysis by forming an aqueous suspension of it at substantially neutral pH, adding approximately 1% by weight of pancreatin with respect to the weight of powder used, maintaining a temperature of 45° to 50° for approximately 24 hours, so as to prepare a lysate; acidifying the lysate with diluted mineral acid, separating liquid from the resulting precipitate and reneutralizing said liquid; adding a sufficient quantity of ammonium sulfate to the reneutralized liquid to reach an ammonium sulfate concentration of approximately 35% of saturation, so as to precipitate the active part of the lysate; collecting the precipitate and forming an aqueous suspension of it at neutral pH; purifying said lysate suspension through selective fractionation operations consisting of adsorption operations; collecting the fractions containing angiokinase, as selected by measuring fibrinolysis activity both in the presence and absence of plasminogen, where said fibrinolytic activity is such that 30 μliters of angiokinase extract form a circular lysis area 3.908 mm in diameter on plates of human fibrin 2.503 mm thick, after 16 hours incubation at 37° C., wherein said product has a protein N content of 0.2 μg/ml and a mean molecular weight of about 18000 dalton.