US4469791A
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US
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Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
Assignee: Genencor International Wisconsin Inc
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Filing: 1981-02-13
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Granted: 1984-09-04
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Expires: 2001-02-13
Abstract
Abstract Genetically engineered microorganisms are provided which contain recombinant DNA with an amylase coding gene. Improved yields of amylase enzymes are obtained by cultivating these microorganisms.
Inventors
- 👤 Charles A. Colson
- 👤 Pierre E. Cornelis
- 👤 Colette S. Digneffe
- 👤 Corinne Walon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:13
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2414; C12N9/2417; C12N9/2425; C12N9/2451; C12N9/2457; C12Y; C12Y302/00; C12Y302/01; C12Y302/01041; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/839; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
36 words
Recombinant DNA containing an amylase or pullulanase gene consisting of a phage selected from the group consisting of NCIB No. 11569, NCIB No. 11572, NCIB No. 11574, NCIB No. 11593, NCIB No. 11604, and mutants thereof.
Claim 2
Dependent Claim
36 words
Recombinant DNA containing an amylase or pullulanase gene consisting of a phage selected from the group consisting of NCIB No. 11569, NCIB No. 11572, NCIB No. 11574, NCIB No. 11593, NCIB No. 11604, and mutants thereof.
Claim 3
Dependent Claim
41 words
A genetically engineered microorganism characterized in that it comprises a phage containing an amylase or pullulanase gene selected from the group consisting of NCIB No. 11569, NCIB No. 11572, NCIB No. 11574, NCIB No. 11593, NCIB No. 11604, and mutants thereof.
Claim 4
Dependent Claim
41 words
A genetically engineered microorganism characterized in that it comprises a phage containing an amylase or pullulanase gene selected from the group consisting of NCIB No. 11569, NCIB No. 11572, NCIB No. 11574, NCIB No. 11593, NCIB No. 11604, and mutants thereof.
Claim 5
Dependent Claim
31 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11586, characterized in that it carries the recombinant phage, NCIB No. 11572, containing a thermostable alpha-amylase coding gene.
Claim 6
Dependent Claim
31 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11586, characterized in that it carries the recombinant phage, NCIB No. 11572, containing a thermostable alpha-amylase coding gene.
Claim 7
Dependent Claim
30 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11603, characterized in that it carries the recombinant phage, NCIB No. 11574, containing a beta-amylase coding gene.
Claim 8
Dependent Claim
30 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11603, characterized in that it carries the recombinant phage, NCIB No. 11574, containing a beta-amylase coding gene.
Claim 9
Dependent Claim
30 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11606, characterized in that it carries the recombinant phage, NCIB No. 11604, containing a pullulanase coding gene.
Claim 10
Dependent Claim
30 words
A biologically pure culture of a lysogenic strain of E. coli, NCIB No. 11606, characterized in that it carries the recombinant phage, NCIB No. 11604, containing a pullulanase coding gene.
Claim 11
Dependent Claim
140 words
An essentially pure plasmid, having an amylase coding gene, selected from the group: pCP 1 having a molecular weight of approximately 6.56 Kb and a restriction endonuclease cleavage map as shown in FIG. 1, pCP 2 having a molecular weight of approximately 7.67 Kb and a restriction endonuclease cleavage map as shown in FIG. 2, pCP 2.3 having a molecular weight of approximately 4.33 Kb and a restriction endonuclease cleavage map as shown in FIG. 3(b), pCH 1 having a molecular weight of approximately 9.67 Kb and a restriction endonuclease cleavage map as shown in FIG. 3(c), pCP 3 having a molecular weight of approximately 7.61 Kb and a restriction endonuclease cleavage map as shown in FIG. 4, and pCP 4 having a molecular weight of approximately 10.1 Kb and a restriction endonuclease cleavage map as shown in FIG. 5.
Claim 12
Dependent Claim
140 words
An essentially pure plasmid, having an amylase coding gene, selected from the group: pCP 1 having a molecular weight of approximately 6.56 Kb and a restriction endonuclease cleavage map as shown in FIG. 1, pCP 2 having a molecular weight of approximately 7.67 Kb and a restriction endonuclease cleavage map as shown in FIG. 2, pCP 2.3 having a molecular weight of approximately 4.33 Kb and a restriction endonuclease cleavage map as shown in FIG. 3(b), pCH 1 having a molecular weight of approximately 9.67 Kb and a restriction endonuclease cleavage map as shown in FIG. 3(c), pCP 3 having a molecular weight of approximately 7.61 Kb and a restriction endonuclease cleavage map as shown in FIG. 4, and pCP 4 having a molecular weight of approximately 10.1 Kb and a restriction endonuclease cleavage map as shown in FIG. 5.
Claim 13
Dependent Claim
46 words
A biologically pure culture of B. subtilis, NCIB No. 11629, characterized in that its cells contain the plasmid, pCH 1, and that it will grow in a medium containing 20 μg of chloramphenicol per ml of medium producing alpha-amylase enzyme which is excreted into the medium.
Claim 14
Dependent Claim
46 words
A biologically pure culture of B. subtilis, NCIB No. 11629, characterized in that its cells contain the plasmid, pCH 1, and that it will grow in a medium containing 20 μg of chloramphenicol per ml of medium producing alpha-amylase enzyme which is excreted into the medium.
Claim 15
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11570, characterized in that its cells contain the plasmid, pCP 1, that it will grow in a medium containing ampicillin and that it will produce an alpha-amylase enzyme.
Claim 16
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11570, characterized in that its cells contain the plasmid, pCP 1, that it will grow in a medium containing ampicillin and that it will produce an alpha-amylase enzyme.
Claim 17
Dependent Claim
38 words
A biologically pure culture of E. coli, NCIB No. 11573, characterized in that its cells contain the plasmid, pCP 2, that it will grow in a medium containing ampicillin and that it will produce a thermostable alpha-amylase enzyme.
Claim 18
Dependent Claim
38 words
A biologically pure culture of E. coli, NCIB No. 11573, characterized in that its cells contain the plasmid, pCP 2, that it will grow in a medium containing ampicillin and that it will produce a thermostable alpha-amylase enzyme.
Claim 19
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11602, characterized in that its cells contain the plasmid, pCP 3, that it will grow in a medium containing ampicillin and that it will produce a beta-amylase enzyme.
Claim 20
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11602, characterized in that its cells contain the plasmid, pCP 3, that it will grow in a medium containing ampicillin and that it will produce a beta-amylase enzyme.
Claim 21
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11605, characterized in that its cells contain the plasmid, pCP 4, that it will grow in a medium containing chloramphenicol and that it will produce a pullulanase enzyme.
Claim 22
Dependent Claim
37 words
A biologically pure culture of E. coli, NCIB No. 11605, characterized in that its cells contain the plasmid, pCP 4, that it will grow in a medium containing chloramphenicol and that it will produce a pullulanase enzyme.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1987-09-04 | 1988-03-05 | 1988-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-04 | 1992-03-05 | 1992-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-04 | 1996-03-05 | 1996-09-04 | $7700.00 | Window Closed / Lapsed |