US4468464A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biologically functional molecular chimeras
Assignee: Leland Stanford Junior University
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Filing: 1978-11-09
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Granted: 1984-08-28
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Expires: 1998-11-09
Abstract
Abstract Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are bound to a gene having complementary termini, to provide a biologically functional replicon with a desired phenotypical property. The replicon is inserted into a microorganism cell by transformation. Isolation of the transformants provides cells for replication and expression of the DNA molecules present in the modified plasmid. The method provides a convenient and efficient way to introduce genetic capability into microorganisms for the production of nucleic acids are proteins, such as medically or commercially useful enzymes, which may have direct usefulness, or may find expression in the production of drugs, such as hormones, antibiotics, or the like, fixation of nitrogen, fermentation, utilization of specific feedstocks, or the like. The invention was supported by generous grants of NIH, NSF and the American Cancer Society.
Inventors
- 👤 Stanley N. Cohen
- 👤 Herbert W. Boyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:48
CPC Classifications
C; C12; C12N; C12N15/00; Y; Y10; Y10S; Y10S435/00; Y10S435/82
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
96 words
As a composition of matter, a biologically functional recombinant plasmid capable of selection and replication in a prokaryotic cell comprising: a first DNA segment containing an intact replicon recognized by said cell derived by cleaving a virus or plasmid compatible with said cell at other than the replicon site, which segment is covalently joined in vitro at its ends to the complementary ends of a second DNA segment foreign to said cell and having at least one intact gene, said second DNA segment derived from a source which does not exchange genetic information with said cell.
Claim 2
Dependent Claim
96 words
As a composition of matter, a biologically functional recombinant plasmid capable of selection and replication in a prokaryotic cell comprising: a first DNA segment containing an intact replicon recognized by said cell derived by cleaving a virus or plasmid compatible with said cell at other than the replicon site, which segment is covalently joined in vitro at its ends to the complementary ends of a second DNA segment foreign to said cell and having at least one intact gene, said second DNA segment derived from a source which does not exchange genetic information with said cell.
Claim 3
Dependent Claim
71 words
As a composition of matter, a biologically functional recombinant plasmid having been cloned at least once and capable of selection and replication, said plasmid having first and second linear segments, wherein said first segment has an intact replicon recognized by a prokaryotic host and said second segment is a gene derived from a source which is foreign to and does not exchange genetic information with a prokaryotic host for said replicon.
Claim 4
Dependent Claim
71 words
As a composition of matter, a biologically functional recombinant plasmid having been cloned at least once and capable of selection and replication, said plasmid having first and second linear segments, wherein said first segment has an intact replicon recognized by a prokaryotic host and said second segment is a gene derived from a source which is foreign to and does not exchange genetic information with a prokaryotic host for said replicon.
Claim 5
Dependent Claim
17 words
A composition of matter according to claim 2, wherein said second segment is derived from eukaryotic source.
Claim 6
Dependent Claim
17 words
A composition of matter according to claim 2, wherein said first segment has a basis for selection.
Claim 7
Dependent Claim
17 words
A composition of matter according to claim 2, wherein said second segment has a basis for selection.
Claim 8
Dependent Claim
72 words
As a composition of matter, a biologically functional recombinant plasmid having been cloned at least once and capable of selection and replication, said plasmid having first and second linear segments, wherein said first segment has an intact replicon derived from a prokaryotic plasmid and said second segment is a gene derived from a source which is foreign to and does not exchange genetic information with a prokaryotic host for said prokaryotic plasmid.
Claim 9
Dependent Claim
72 words
As a composition of matter, a biologically functional recombinant plasmid having been cloned at least once and capable of selection and replication, said plasmid having first and second linear segments, wherein said first segment has an intact replicon derived from a prokaryotic plasmid and said second segment is a gene derived from a source which is foreign to and does not exchange genetic information with a prokaryotic host for said prokaryotic plasmid.
Claim 10
Dependent Claim
18 words
A composition of matter according to claim 6, wherein said second segment is derived from a eukaryotic source.
Claim 11
Dependent Claim
20 words
A composition of matter according to claim 6, wherein the basis for selection is resistance to a growth inhibiting substance.
Claim 12
Dependent Claim
20 words
A composition of matter according to claim 2, wherein the basis for selection is resistance to a growth inhibiting substance.
Claim 13
Dependent Claim
16 words
A composition of matter according to claim 8, wherein said growth inhibiting substance is an antibiotic.
Claim 14
Dependent Claim
16 words
A composition of matter according to claim 9, wherein said growth inhibiting substance is an antibiotic.
References Cited (Backward Citations)
US4237224A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-28 | 1988-02-27 | 1988-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-28 | 1992-02-27 | 1992-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-28 | 1996-02-27 | 1996-08-28 | $7700.00 | Window Closed / Lapsed |