US4468346A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Monoclonal antibodies to porcine immunoglobulins
Assignee: UNITED STATES AGRICULTURE THE, Secretary of
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Filing: 1983-10-27
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Granted: 1984-08-28
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Expires: 2003-10-27
Abstract
Abstract Monoclonal antibodies which are heavy-chain specific for porcine Ig's are secreted by novel hybrid cell lines produced by fusing SP2/O myeloma cells with B-lymphocytes from BALB/c mice immunized against porcine Ig's. The monoclonal antibodies are useful in porcine immunological research and pathological diagnosis.
Inventors
- 👤 Prem S. Paul
- 👤 Richard A. Van Deusen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/827; Y10S530/829; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
9 words
Monoclonal antibody which is heavy-chain specific for porcine immunoglobulin.
Claim 2
Dependent Claim
9 words
Monoclonal antibody which is heavy-chain specific for porcine immunoglobulin.
Claim 3
Dependent Claim
11 words
Monoclonal antibody as described in claim 1 which is μ-chain specific.
Claim 4
Dependent Claim
11 words
Monoclonal antibody as described in claim 1 which is γ-chain specific.
Claim 5
Dependent Claim
11 words
Monoclonal antibody as described in claim 1 which is α-chain specific.
Claim 6
Dependent Claim
27 words
Monoclonal antibody as described in claim 1 produced by a hybridoma generated by fusing mouse myeloma cells with splenic B-lymphocytes from a mouse immunized with porcine immunoglobulin.
Claim 7
Dependent Claim
28 words
Monoclonal antibody as described in claim 1 produced by a hybridoma generated by fusing SP2/0 myeloma cells with splenic lymphocytes from a BALB/c mouse immunized with porcine immunoglobulin.
Claim 8
Independent Claim
51 words
A method of preparing monoclonal antibody which is heavy-chain specific for a predetermined porcine immunoglobulin type comprising culturing in vitro a hybridoma generated by fusing mouse myeloma cells with splenic B-lymphocytes from a mouse immunized with said porcine immunoglobulin type, and collecting from said culture the supernatant fluid containing the antibody.
Claim 9
Independent Claim
51 words
A method of preparing monoclonal antibody which is heavy-chain specific for a predetermined porcine immunoglobulin type comprising culturing in vitro a hybridoma generated by fusing mouse myeloma cells with splenic B-lymphocytes from a mouse immunized with said porcine immunoglobulin type, and collecting from said culture the supernatant fluid containing the antibody.
Claim 10
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 7 wherein said immunoglobulin type is IgM and said antibody is γ-chain specific.
Claim 11
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 7 wherein said immunoglobulin type is IgG and said antibody is β-chain specific.
Claim 12
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 7 wherein said immunoglobulin type is IgA and said antibody is α-chain specific.
Claim 13
Dependent Claim
9 words
Monoclonal antibody prepared by the method of claim 7.
Claim 14
Independent Claim
53 words
A method of preparing monoclonal antibody which is heavy-chain specific for a predetermined porcine immunoglobulin type comprising injecting a mouse intraperitoneally with a hybridoma generated by fusing mouse myeloma cells with splenic lymphocytes from a mouse immunized with said porcine immunoglobulin type, and collecting from said mouse the ascites fluid containing the antibody.
Claim 15
Independent Claim
53 words
A method of preparing monoclonal antibody which is heavy-chain specific for a predetermined porcine immunoglobulin type comprising injecting a mouse intraperitoneally with a hybridoma generated by fusing mouse myeloma cells with splenic lymphocytes from a mouse immunized with said porcine immunoglobulin type, and collecting from said mouse the ascites fluid containing the antibody.
Claim 16
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 12 wherein said immunoglobulin type is IgM and said antibody is μ-chain specific.
Claim 17
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 12 wherein said immunoglobulin type is IgG and said antibody is γ-chain specific.
Claim 18
Dependent Claim
23 words
A method of preparing monoclonal antibody as described in claim 12 wherein said immunoglobulin type is IgA and said antibody is α-chain specific.
Claim 19
Dependent Claim
9 words
Monoclonal antibody prepared by the method of claim 12.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-28 | 1988-02-27 | 1988-08-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-28 | 1992-02-27 | 1992-08-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-28 | 1996-02-27 | 1996-08-28 | $7700.00 | Window Closed / Lapsed |