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US4465776A
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Monoclonal antibodies to vitamin B6 and immunoassay method
Assignee: RESEARCH Corp A NEW YORK NOT-FOR-PROFIT CORP
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Filing: 1982-09-27
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Granted: 1984-08-14
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Expires: 2002-09-27
Abstract
Abstract A continuous hybridoma cell line which secretes recoverable quantities of monoclonal antibodies having specificity against Vitamin B 6 , which antibodies are useful in a method for detecting the presence of vitamin B 6 in an animal sample.
Inventors
- 👤 John A. Cidlowski
- 👤 Dace Viceps-Madore
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:18
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/82; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/948; Y10S435/975; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/81; Y10S436/815; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
16 words
A murine monoclonal antibody of the IgG class which is specific for vitamin B 6 .
Claim 2
Dependent Claim
16 words
A murine monoclonal antibody of the IgG class which is specific for vitamin B 6 .
Claim 3
Dependent Claim
24 words
The antibody of claim 1 wherein said vitamin B 6 is selected from the group consisting of pyridoxal, pyridoxine, pyridoxamine and phosphate esters thereof.
Claim 4
Dependent Claim
15 words
The antibody of claim 1 wherein said vitamin B 6 is the biologically active form.
Claim 5
Dependent Claim
9 words
The antibody of claim 1 in detectably labeled form.
Claim 6
Dependent Claim
18 words
The antibody of claim 4 wherein said label is an enzyme label, a chromophore label or a radiolabel.
Claim 7
Dependent Claim
12 words
The antibody of claim 1 which is immobilized on a solid phase.
Claim 8
Dependent Claim
14 words
The antibody of claim 6 wherein said solid phase is plastic, glass or latex.
Claim 9
Dependent Claim
18 words
The antibody of claim 6 wherein said solid phase is a slide, a tube or a plastic strip.
Claim 10
Dependent Claim
68 words
A kit useful for the detection of vitamin B 6 in a sample which comprises: a carrier being compartmentalized to receive one or more container means in close confinement therein, a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B 6 ; a second container means comprising a detectably labeled antibody which is specific for vitamin B 6 .
Claim 11
Dependent Claim
68 words
A kit useful for the detection of vitamin B 6 in a sample which comprises: a carrier being compartmentalized to receive one or more container means in close confinement therein, a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B 6 ; a second container means comprising a detectably labeled antibody which is specific for vitamin B 6 .
Claim 12
Dependent Claim
62 words
A kit useful for the detection of vitamin B 6 in a sample which comprises: a carrier being compartmentalized to receive one or more container means in close confinement therein, a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B 6 ; a second container means comprising detectably labeled vitamin B 6 .
Claim 13
Dependent Claim
62 words
A kit useful for the detection of vitamin B 6 in a sample which comprises: a carrier being compartmentalized to receive one or more container means in close confinement therein, a first container means comprising a murine monoclonal antibody of the IgG class which is specific for vitamin B 6 ; a second container means comprising detectably labeled vitamin B 6 .
Claim 14
Dependent Claim
24 words
The kit of any of claims 9 or 10 wherein said monoclonal antibody in said first container means is immobilized on said container means.
Claim 15
Dependent Claim
23 words
The kit of claim 9 wherein said antibody in said second container is labeled with a radiolabel, an enzyme label or a chromophore.
Claim 16
Dependent Claim
17 words
The kit of claim 9 wherein said antibody in said second container means is a monoclonal antibody.
Claim 17
Dependent Claim
25 words
The kit of any of claims 9 or 10 which also comprises a multiplicity of container means with different amounts of vitamin B 6 .
Claim 18
Dependent Claim
19 words
The kit of any of claims 9 or 10 wherein said vitamin B 6 is the biologically form thereof.
Claim 19
Dependent Claim
16 words
The kit of any of claims 9 or 10 wherein said container means is a tube.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-14 | 1988-02-13 | 1988-08-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-14 | 1992-02-13 | 1992-08-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-14 | 1996-02-13 | 1996-08-14 | $7700.00 | Window Closed / Lapsed |