US4464295A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Simple and rapid method for extraction of proteins from bacteria
Assignee: United States, AGRICULTURE THE, Secretary of, Department of
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Filing: 1983-08-17
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Granted: 1984-08-07
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Expires: 2003-08-17
Abstract
Abstract 44A simple, rapid method for extracting proteins from pathogenic and nonpathogenic bacteria is described.
Inventors
- 👤 Saumya Bhaduri
- 👤 Paul H. Demchick
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 09:24
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/008; C; C12; C12N; C12N1/00; C12N1/005; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
58 words
A method of extracting proteins from bacteria comprising treating bacterial cells with a suitable volatile organic solvent for about five minutes, centrifuging the treated cells and the solvent to collect the cells, removing the volatile residues from the collected cells with a stream of inert gas, and extracting the proteins from the cells with an aqueous phase solvent.
Claim 2
Independent Claim
58 words
A method of extracting proteins from bacteria comprising treating bacterial cells with a suitable volatile organic solvent for about five minutes, centrifuging the treated cells and the solvent to collect the cells, removing the volatile residues from the collected cells with a stream of inert gas, and extracting the proteins from the cells with an aqueous phase solvent.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the solvent is acetone and the aqueous phase solvent is sodium dodecyl sulfate.
Claim 4
Independent Claim
42 words
A method of extracting proteins from bacteria comprising suspending the bacterial cells in ice-cold acetone for about five minutes, centrifuging the suspension to collect the cells, removing residual acetone with a stream of nitrogen, and extracting the protein with sodium dodecyl sulfate.
Claim 5
Independent Claim
42 words
A method of extracting proteins from bacteria comprising suspending the bacterial cells in ice-cold acetone for about five minutes, centrifuging the suspension to collect the cells, removing residual acetone with a stream of nitrogen, and extracting the protein with sodium dodecyl sulfate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-07 | 1988-02-06 | 1988-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-07 | 1992-02-06 | 1992-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-07 | 1996-02-06 | 1996-08-07 | $7700.00 | Window Closed / Lapsed |