US4460692A
Expired / Lapsed
US
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Microemulsion of nutrient substances
Assignee: Societe Nationale Elf Aquitaine Production SA
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Filing: 1983-03-03
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Granted: 1984-07-17
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Expires: 2003-03-03
Abstract
Abstract There is disclosed a nutrient composition to be added to a hydrophobic medium for culturing microorganisms comprising a micro-emulsion of an aqueous solution of nutrient material in a liquid immiscible with water. As the source of nitrogen, compounds soluble in water are used, such as urea or ammonium nitrate, sulphate or phosphate, and, as the source of phosphorus, compounds soluble in water are used. The medium sould also contain a directly-assimilable source of carbon. Application of this composition for culturing of microorganisms allows operations to be carried out which comprise the degrading of hydrocabons covering an area of water or ground; an application of particular interest for operation is for combatting oil pollution of the sea by biological degradation. As the source of nitrogen, compounds soluble in water are used, such as urea or ammonium nitrate, sulphate or phosphate, and, as the source of phosphorus, compounds soluble in water are used. The medium should also contain a directly-assimilable source of carbon. Application of this composition for culturing of microorganisms allows operations to be carried out which comprise the degrading of hydrocarbons covering an area of water or ground; an application of particular interest for operations is for combatting oil pollution of the sea by biological degradation.
Inventors
- 👤 Jacques Tellier
- 👤 Andree Sirvins
- 👤 Jean-Claude Gautier
- 👤 Bernard Tramier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:11
CPC Classifications
C; C12; C12N; C12N1/00; C02; C02F; C02F3/00; C02F3/34; C02F3/344; C12N1/26
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
106 words
A nutrient liquid composition intended to be added to a hydrophobic medium for the culture of microorganisms therein comprising a microemulsion of an aqueous solution of nutrient material in a liquid immiscible with water, the aqueous solution being the internal phase of the microemulsion, said microemulsion comprising by weight 10-30% of water, 4-10% of microorganism assimilable nitrogen in the form of nitrogenous compounds, 5-35% of a 10-18 carbon atom alkyl or alkenyl phosphate or an epoxylated phenol alkyl phosphate, 0-20% of an alkyl ether of an alkylene glycol and 20-50% of a fatty acid ester, acid or alcohol or mixture thereof with a petroleum derivative hydrocarbon.
Claim 2
Dependent Claim
106 words
A nutrient liquid composition intended to be added to a hydrophobic medium for the culture of microorganisms therein comprising a microemulsion of an aqueous solution of nutrient material in a liquid immiscible with water, the aqueous solution being the internal phase of the microemulsion, said microemulsion comprising by weight 10-30% of water, 4-10% of microorganism assimilable nitrogen in the form of nitrogenous compounds, 5-35% of a 10-18 carbon atom alkyl or alkenyl phosphate or an epoxylated phenol alkyl phosphate, 0-20% of an alkyl ether of an alkylene glycol and 20-50% of a fatty acid ester, acid or alcohol or mixture thereof with a petroleum derivative hydrocarbon.
Claim 3
Dependent Claim
11 words
Composition according to claim 1, wherein the nitrogenous compound comprises urea.
Claim 4
Dependent Claim
21 words
Composition according to claim 2, wherein the urea is present in the aqueous solution at a concentration of 10-60% by weight.
Claim 5
Dependent Claim
13 words
Composition according to claim 3, wherein said urea solution contains an amino acid.
Claim 6
Dependent Claim
17 words
Composition according to claim 4, in which the urea nitrogen is 50-99% of the total nitrogen present.
Claim 7
Dependent Claim
36 words
Composition according to claim 5, in which the amino acid is at least one member of the group consisting of glycine, alamine, serine, cysteine, valine, glutamine, leucine, lysine, argynine, proline, tyrosine, aspartic acid and glutamic acid.
Claim 8
Dependent Claim
35 words
Composition according to claim 5, in which said microemulsion contains 20-50% of a mixture of a fatty ester, acid or alcohol with a petroleum derivative hydrocarbon in which the petroleum derivative hydrocarbon is 5-90% thereof.
Claim 9
Dependent Claim
27 words
Composition according to claim 1, wherein the nitrogenous compound is at least one member selected from the group consisting of ammonium sulphate, ammonium phosphate, lecithin and urea.
Claim 10
Dependent Claim
19 words
Composition according to claim 8, in which at least two nitrogenous compounds are present, one of which is urea.
Claim 11
Dependent Claim
35 words
Composition according to claim 1, in which said microemulsion contains 20-50% of a mixture of a fatty ester, acid or alcohol with a petroleum derivative hydrocarbon in which the petroleum derivative hydrocarbon is 5-90% thereof.
Claim 12
Dependent Claim
25 words
Composition according to claim 1, in which the external phase of the microemulsion is a lipomiscible organic liquid containing compounds of carbon assimilable by microorganisms.
Claim 13
Dependent Claim
18 words
Composition according to claim 1, in which the phosphorus to nitrogen weight ratio is from 0.02 to 0.2.
Claim 14
Dependent Claim
13 words
Composition according to claim 12, wherein said ratio is from 0.05 to 0.15.
Claim 15
Dependent Claim
11 words
Composition according to claim 13, wherein said ratio is about 0.05.
Claim 16
Dependent Claim
10 words
Composition according to claim 1 containing 5-8% of assimilable nitrogen.
Claim 17
Dependent Claim
17 words
Composition according to claim 1 in which the fatty acid ester, acid or alcohol comprises oleic acid.
Claim 18
Dependent Claim
17 words
Composition according to claim 1 in which said microemulsion comprises water, urea, oleic acid and oleyl phosphate.
Claim 19
Dependent Claim
21 words
Composition according to claim 1, wherein said microemulsion comprises water, urea, oleic acid, butyl ether of ethylene glycol and lauryl phosphate.
Claim 20
Dependent Claim
27 words
Composition according to claim 1, wherein said microemulsion comprises 13.8-19.2% water, 13.8-15.7% urea, 28-36.8% oleic acid, 10.5-10.6% butyl ether of ethylene glycol and 19.5-25% of lauryl phosphate.
Claim 21
Dependent Claim
17 words
Composition according to claim 1 in which the internal phase is droplets of 80-600 Angstroms in diameter.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-17 | 1988-01-16 | 1988-07-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-17 | 1992-01-16 | 1992-07-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-17 | 1996-01-16 | 1996-07-17 | $7700.00 | Window Closed / Lapsed |