US4457865A
Expired / Lapsed
US
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Sialic acid specific slug lectin
Assignee: RESEARCH Corp 405 LEXINGTON AVE NEW YORK NY 10174 A NEW YORK NOT-FOR-PROFIT CORPORATION
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Filing: 1982-03-08
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Granted: 1984-07-03
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Expires: 2002-03-08
Abstract
Abstract A hemagglutinate lectin derived from the slug, Limax flavus, having a high degree of hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoproteins and diagnostic and separatory methods and compositions based upon the inhibitory effect of sialoproteins or sialic acid on the hemagglutinating properties of the slug-derived lectin.
Inventors
- 👤 Ronald L. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:37
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S530/00; Y10S530/855
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
76 words
The substantially homogeneous lectin of the slug, Limax flavus having a hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoprotein, having an approximate amino acid composition: ______________________________________
Moles per 22,000 g of lectin
Amino Acid (Nearest Integer)
______________________________________
Asx 26
Thr 13
Ser 13
Glx 14
Pro 4
Gly 22
Ala 16
Val 7
Met 2
Ile 7
Leu 15
Tyr 10
Phe 10
His 7
Lys 17
Trp 6
Arg 8
Cys 6
______________________________________
Claim 2
Dependent Claim
76 words
The substantially homogeneous lectin of the slug, Limax flavus having a hemagglutinative inhibitory specificity and selectivity for sialic acid or sialoprotein, having an approximate amino acid composition: ______________________________________
Moles per 22,000 g of lectin
Amino Acid (Nearest Integer)
______________________________________
Asx 26
Thr 13
Ser 13
Glx 14
Pro 4
Gly 22
Ala 16
Val 7
Met 2
Ile 7
Leu 15
Tyr 10
Phe 10
His 7
Lys 17
Trp 6
Arg 8
Cys 6
______________________________________
Claim 3
Dependent Claim
27 words
The lectin of claim 1 isolated from the slug, Limax flavus, by homogenization of eviscerated tissue, ammonium sulfate fractionation and affinity chromatography on sialic acid containing protein.
Claim 4
Dependent Claim
30 words
The lectin of claim 1 isolated by homogenization in saline solution fractionation with 40% and 80% ammonium sulfate and affivity chromatography on bovine submaxillary mucin coupled to an inert substrate.
Claim 5
Dependent Claim
40 words
A method for isolating a sialopeptide or a sialoprotein from a biological liquid comprising contacting said biological liquid with the lectin of claim 1 to form a lectin-sialic acid or lectin-sialoprotein complex and separating said complex from said biological liquid.
Claim 6
Dependent Claim
18 words
The method of claim 4 including the subsequent step of separating said sialopeptide or sialoprotein from said complex.
Claim 7
Dependent Claim
20 words
The method of claim 4 wherein said sialic acid or sialoprotein is separated from said complex by polyacrylamide gel electrophoresis.
Claim 8
Dependent Claim
35 words
The method of claim 4 wherein said lectin is bound to a substrate inert to said biological liquid and suitable for affinity chromatography and said biological liquid is contacted therewith in an affinity chromatographic column.
Claim 9
Dependent Claim
23 words
The method of claim 7 wherein said sialopeptide or sialoprotein is eluted from said substrate by washing with a liquid containing sialic acid.
Claim 10
Dependent Claim
46 words
A composition suitable for the diagnostic determination of the sialic acid content of the biological liquid of a mammal comprising an aqueous solution or suspension of an amount of the lectin of claim 1 sufficient to exert a measurable hemagglutinate effect on the biological liquid sample.
Claim 11
Dependent Claim
14 words
The composition of claim 9 wherein said aqueous solution or suspension comprises isotonic saline.
Claim 12
Dependent Claim
43 words
The composition of claim 9 containing an amount of said lectin sufficient to exert a hemagglutinate effect measurable by determining the optical density of the lectin treated biological liquid sample and comparison thereof with the optical density of an untreated biological liquid sample.
References Cited (Backward Citations)
US4115062A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-07-03 | 1988-01-02 | 1988-07-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-07-03 | 1992-01-02 | 1992-07-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-07-03 | 1996-01-02 | 1996-07-03 | $7700.00 | Window Closed / Lapsed |