Abstract In the enzyme-mediated synthesis of an ester or lactone from at least one carboxylic acid of the formula R-CH2-CH2-COOH wherein R is a hydrogen atom, or a hydrocarbon radical which has 1 to 21 carbon atoms and which can be optionally substituted by hydroxyl groups or alkoxy groups with 1 to 10 carbon atoms, with at least one primary or secondary alcohol having 1 to 15 carbon atoms, the improvement which comprises employing as the enzyme the esterase from Mucor miehei and effecting the synthesis in the absence of water. Advantageously, the synthesis is effected at about room temperature, the mol ratio of carboxylic acid to alcohol is from about 1:1 to 1:1.1, and the enzyme is used in a quantity of about 3 to 20% by weight of the carboxylic acid. The absence of water facilitates recovery, as by distillation.
In the enzyme-mediated synthesis of an ester or lactone of at least one carboxylic acid of the formula R--CH.sub.2 --CH.sub.2 --COOH wherein R is a hydrogen atom, or a hydrocarbon radical which has 1 to 21 carbon atoms and which can be substituted by hydroxyl groups or alkoxyl groups with 1 to 10 carbon atoms, wherein such acid is reacted with at least one primary or secondary alcohol having from 1 to 15 carbon atoms in the presence of an esterase derived from Mucor miehei the improvement comprising effecting the reaction in the absence of water other than that formed in the reaction.
Claim 2
Dependent Claim
103 words
In the enzyme-mediated synthesis of an ester or lactone of at least one carboxylic acid of the formula R--CH.sub.2 --CH.sub.2 --COOH wherein R is a hydrogen atom, or a hydrocarbon radical which has 1 to 21 carbon atoms and which can be substituted by hydroxyl groups or alkoxyl groups with 1 to 10 carbon atoms, wherein such acid is reacted with at least one primary or secondary alcohol having from 1 to 15 carbon atoms in the presence of an esterase derived from Mucor miehei the improvement comprising effecting the reaction in the absence of water other than that formed in the reaction.
Claim 3
Dependent Claim
20 words
A process according to claim 1, wherein the synthesis is effected at a temperature from about 0° to 50° C.
Claim 4
Dependent Claim
21 words
A process according to claim 1, wherein the mol ratio of carboxylic acid to alcohol is from about 2:1 to 1:6.
Claim 5
Dependent Claim
25 words
A process according to claim 1, wherein the enzyme is used in a quantity of about 1 to 30% by weight of the carboxylic acid.
Claim 6
Dependent Claim
48 words
A process according to claim 1, wherein the synthesis is effected at about room temperature, the mol ratio of carboxylic acid to alcohol is from about 1:1 to 1:1.1, and the enzyme is used in a quantity of about 3 to 20% by weight of the carboxylic acid.